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The Resource 2-D proteome analysis protocols, edited by Andrew J. Link

2-D proteome analysis protocols, edited by Andrew J. Link

Contributor
Summary
In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions. 2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery
Language
eng
Work
Publication
Extent
1 online resource (xvii, 601 pages)
Contents
  • 2-D protein gel electrophoresis. An overview / J. Fichmann and R. Westermeier
  • Solubilization of proteins in 2-D electrophoresis. An outline / T. Rabilloud
  • Preparation of escherichia coli samples for 2-D gel analysis / R.A. VanBogelen and F.C. Neidhardt
  • Preparing 2-D protein extracts from yeast / D.M. Schieltz
  • 2-D protein extracts from drosophila melanogaster / C. Ericsson
  • Preparing 2-D protein extracts from caenorhabditis elegans / R. Zwilling
  • Eukaryotic cell labeling and preparation for 2-D / N. Bizios
  • Differential detergent fractionation of eukaryotic cells. Analysis by two-dimensional gel electrophoresis / M.L. Ramsby and G.S. Makowski
  • Fractionated extraction of total tissue proteins from mouse and human for 2-D electrophoresis / J. Klose
  • Preparation and solubilization of body fluids for 2-D / J.C. Sanchez and D.F. Hochstrasser
  • 2-D electrophoresis of plant proteins / A. Tsugita and M. Kamo
  • Quantifying protein in 2-D page solubilization buffers / L.S. Ramagli
  • Measuring the radioactivity of 2-D protein extracts / A.J. Link and N. Bizios
  • Advantages of carrier ampholyte ief / M.F. Lopez
  • 2-D electrophoresis using carrier ampholytes in the first dimension (ief) / M.F. Lopez
  • Nonequilibrium ph gel electrophoresis (nephge) / M.F. Lopez
  • High-resolution, 2-D protein electrophoresis using nondedicated equipment / M. Sarmiento
  • Large-gel 2-D electrophoresis / J. Klose
  • Advantages of immobilized ph gradients / J. Fichmann
  • Casting immobilized ph gradients (ipgs) / E. Gianazza
  • Analytical ipg-dalt / A. Gorg and W. Weiss
  • Ipg-dalt of very alkaline proteins / A. Gorg
  • Running preparative carrier ampholyte and immobilized ph gradient ief gels for 2-D / N.M. Matsui [and others]
  • In-gel sample rehydration of immobilized ph gradient / J.C. Sanchez, D. Hochstrasser and T. Rabilloud
  • High-resolution, ipg-based, mini two-dimensional gel electrophoresis / J.C. Sanchez and D.F. Hochstrasser
  • Horizontal sds-page for ipg-dalt / A. Gorg and W. Weiss
  • Casting and running vertical slab-gel electrophoresis for 2D-page / B.J. Walsh and B.R. Herbert
  • Nonreducing 2-D polyacrylamide gel electrophoresis / H. Ji and R.J. Simpson
  • 2-D diagonal gel electrophoresis / J. Goverman
  • 2-D phosphopeptide mapping / H. Nagahara [and others]
  • Internal standards for 2-D / A.J. Link
  • Autoradiography of 2-D gels / A.J. Link
  • Double-label analysis / K.H. Lee and M.G. Harrington
  • Silver staining of 2-D electrophoresis gels / T. Rabilloud
  • Staining of preparative 2-D gels. Coomassie blue and imidazole-zinc negative staining / N.M. Matsui, D.M. Smith-Beckerman and L.B. Epstein
  • Electroblotting of proteins from 2-D polyacrylamide gels / M.J. Dunn
  • Detection of total proteins on western blots of 2-D polyacrylamide gels / M.J. Dunn
  • Protein detection using reversible metal chelate stains / W.F. Patton, M.J. Lim and D. Shepro
  • Glycoprotein detection of 2-D separated proteins / N.H. Packer, M.S. Ball and P.L. Devine
  • Image acquisition in 2-D electrophoresis / W.F. Patton, M.J. Lim and D. Shepro
  • Computer analysis of 2-D images / R.D. Appel and D.F. Hochstrasser
  • 2-D databases on the World Wide Web / R.D. Appel, A. Bairoch and D.F. Hochstrasser
  • Comparing 2-D electrophoretic gels across internet databases / P.F. Lemkin
  • Constructing a 2-D database for the World Wide Web / R.D. Appel [and others]
  • Absolute quantitation of 2-D protein spots / S.P. Gygi and R. Aebersold
  • Generating a bacterial genome inventory. Identifying 2-D spots by comigrating products of the genome on 2-D gels / R.A. VanBogelen
  • Immunoaffinity identification of 2-De separated proteins / B. Magi [and others]
  • 2-De spot amino acid analysis with 9-fluorenylmethyl chloroformate / M.R. Wilkins, J.X. Yan and A.A. Gooley
  • N-terminal amino acid sequencing of 2-De spots / M. Kamo and A. Tsugita
  • Characterizing proteins from 2-De gels by internal sequence analysis of peptide fragments. Strategies for microsample handling / H. Erdjument-Bromage [and others]
  • Obtaining molecular weights of proteins and their cleavage products by directly combining gel electrophoresis with mass spectrometry / R.R. Ogorzalek Loo, J.A. Loo and P.C. Andrews
  • Identification of proteins by matrix-assisted laser desorption/ionization mass spectrometry using peptide and fragment ion masses / P.L. Courchesne and S.D. Patterson
  • Sample preparation methods for mass spectrometric peptide mapping directly from 2-De gels / O.N. Jensen [and others]
  • Protein identification and analysis tools in the expasy server / M.R. Wilkins [and others]
  • Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry / J.R. Yates, 3rd [and others]
  • Peptide sequencing of 2-De gel-isolated proteins by nanoelectrospray tandem mass spectrometry / O.N. Jensen [and others]
Isbn
9781592595846
Instance
Label
2-D proteome analysis protocols
Title
2-D proteome analysis protocols
Statement of responsibility
edited by Andrew J. Link
Title variation
Two-D proteome analysis protocols
Contributor
Subject
Language
eng
Summary
In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions. 2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery
Member of
Action
digitized
Cataloging source
N$T
Dewey number
572/.6
Illustrations
illustrations
Index
index present
LC call number
QP551
LC item number
.A14 1999eb
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.112
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.112 1999
  • Z9 1999
http://library.link/vocab/relatedWorkOrContributorName
Link, Andrew J
Series statement
Methods in molecular biology
Series volume
v. 112
http://library.link/vocab/subjectName
  • Proteins
  • Gel electrophoresis
  • Gene expression
  • Proteins
  • Electrophoresis, Agar Gel
  • Gene Expression
  • SCIENCE
  • Gel electrophoresis
  • Gene expression
  • Proteins
  • Proteomen
  • Genexpressie
  • Karakterisering (algemeen)
  • Elektroforese
Label
2-D proteome analysis protocols, edited by Andrew J. Link
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
2-D protein gel electrophoresis. An overview / J. Fichmann and R. Westermeier -- Solubilization of proteins in 2-D electrophoresis. An outline / T. Rabilloud -- Preparation of escherichia coli samples for 2-D gel analysis / R.A. VanBogelen and F.C. Neidhardt -- Preparing 2-D protein extracts from yeast / D.M. Schieltz -- 2-D protein extracts from drosophila melanogaster / C. Ericsson -- Preparing 2-D protein extracts from caenorhabditis elegans / R. Zwilling -- Eukaryotic cell labeling and preparation for 2-D / N. Bizios -- Differential detergent fractionation of eukaryotic cells. Analysis by two-dimensional gel electrophoresis / M.L. Ramsby and G.S. Makowski -- Fractionated extraction of total tissue proteins from mouse and human for 2-D electrophoresis / J. Klose -- Preparation and solubilization of body fluids for 2-D / J.C. Sanchez and D.F. Hochstrasser -- 2-D electrophoresis of plant proteins / A. Tsugita and M. Kamo -- Quantifying protein in 2-D page solubilization buffers / L.S. Ramagli -- Measuring the radioactivity of 2-D protein extracts / A.J. Link and N. Bizios -- Advantages of carrier ampholyte ief / M.F. Lopez -- 2-D electrophoresis using carrier ampholytes in the first dimension (ief) / M.F. Lopez -- Nonequilibrium ph gel electrophoresis (nephge) / M.F. Lopez -- High-resolution, 2-D protein electrophoresis using nondedicated equipment / M. Sarmiento -- Large-gel 2-D electrophoresis / J. Klose -- Advantages of immobilized ph gradients / J. Fichmann -- Casting immobilized ph gradients (ipgs) / E. Gianazza -- Analytical ipg-dalt / A. Gorg and W. Weiss -- Ipg-dalt of very alkaline proteins / A. Gorg -- Running preparative carrier ampholyte and immobilized ph gradient ief gels for 2-D / N.M. Matsui [and others] -- In-gel sample rehydration of immobilized ph gradient / J.C. Sanchez, D. Hochstrasser and T. Rabilloud -- High-resolution, ipg-based, mini two-dimensional gel electrophoresis / J.C. Sanchez and D.F. Hochstrasser -- Horizontal sds-page for ipg-dalt / A. Gorg and W. Weiss -- Casting and running vertical slab-gel electrophoresis for 2D-page / B.J. Walsh and B.R. Herbert -- Nonreducing 2-D polyacrylamide gel electrophoresis / H. Ji and R.J. Simpson -- 2-D diagonal gel electrophoresis / J. Goverman -- 2-D phosphopeptide mapping / H. Nagahara [and others] -- Internal standards for 2-D / A.J. Link -- Autoradiography of 2-D gels / A.J. Link -- Double-label analysis / K.H. Lee and M.G. Harrington -- Silver staining of 2-D electrophoresis gels / T. Rabilloud -- Staining of preparative 2-D gels. Coomassie blue and imidazole-zinc negative staining / N.M. Matsui, D.M. Smith-Beckerman and L.B. Epstein -- Electroblotting of proteins from 2-D polyacrylamide gels / M.J. Dunn -- Detection of total proteins on western blots of 2-D polyacrylamide gels / M.J. Dunn -- Protein detection using reversible metal chelate stains / W.F. Patton, M.J. Lim and D. Shepro -- Glycoprotein detection of 2-D separated proteins / N.H. Packer, M.S. Ball and P.L. Devine -- Image acquisition in 2-D electrophoresis / W.F. Patton, M.J. Lim and D. Shepro -- Computer analysis of 2-D images / R.D. Appel and D.F. Hochstrasser -- 2-D databases on the World Wide Web / R.D. Appel, A. Bairoch and D.F. Hochstrasser -- Comparing 2-D electrophoretic gels across internet databases / P.F. Lemkin -- Constructing a 2-D database for the World Wide Web / R.D. Appel [and others] -- Absolute quantitation of 2-D protein spots / S.P. Gygi and R. Aebersold -- Generating a bacterial genome inventory. Identifying 2-D spots by comigrating products of the genome on 2-D gels / R.A. VanBogelen -- Immunoaffinity identification of 2-De separated proteins / B. Magi [and others] -- 2-De spot amino acid analysis with 9-fluorenylmethyl chloroformate / M.R. Wilkins, J.X. Yan and A.A. Gooley -- N-terminal amino acid sequencing of 2-De spots / M. Kamo and A. Tsugita -- Characterizing proteins from 2-De gels by internal sequence analysis of peptide fragments. Strategies for microsample handling / H. Erdjument-Bromage [and others] -- Obtaining molecular weights of proteins and their cleavage products by directly combining gel electrophoresis with mass spectrometry / R.R. Ogorzalek Loo, J.A. Loo and P.C. Andrews -- Identification of proteins by matrix-assisted laser desorption/ionization mass spectrometry using peptide and fragment ion masses / P.L. Courchesne and S.D. Patterson -- Sample preparation methods for mass spectrometric peptide mapping directly from 2-De gels / O.N. Jensen [and others] -- Protein identification and analysis tools in the expasy server / M.R. Wilkins [and others] -- Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry / J.R. Yates, 3rd [and others] -- Peptide sequencing of 2-De gel-isolated proteins by nanoelectrospray tandem mass spectrometry / O.N. Jensen [and others]
Control code
170925862
Dimensions
unknown
Extent
1 online resource (xvii, 601 pages)
File format
unknown
Form of item
online
Isbn
9781592595846
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592595847
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)170925862
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
2-D proteome analysis protocols, edited by Andrew J. Link
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
2-D protein gel electrophoresis. An overview / J. Fichmann and R. Westermeier -- Solubilization of proteins in 2-D electrophoresis. An outline / T. Rabilloud -- Preparation of escherichia coli samples for 2-D gel analysis / R.A. VanBogelen and F.C. Neidhardt -- Preparing 2-D protein extracts from yeast / D.M. Schieltz -- 2-D protein extracts from drosophila melanogaster / C. Ericsson -- Preparing 2-D protein extracts from caenorhabditis elegans / R. Zwilling -- Eukaryotic cell labeling and preparation for 2-D / N. Bizios -- Differential detergent fractionation of eukaryotic cells. Analysis by two-dimensional gel electrophoresis / M.L. Ramsby and G.S. Makowski -- Fractionated extraction of total tissue proteins from mouse and human for 2-D electrophoresis / J. Klose -- Preparation and solubilization of body fluids for 2-D / J.C. Sanchez and D.F. Hochstrasser -- 2-D electrophoresis of plant proteins / A. Tsugita and M. Kamo -- Quantifying protein in 2-D page solubilization buffers / L.S. Ramagli -- Measuring the radioactivity of 2-D protein extracts / A.J. Link and N. Bizios -- Advantages of carrier ampholyte ief / M.F. Lopez -- 2-D electrophoresis using carrier ampholytes in the first dimension (ief) / M.F. Lopez -- Nonequilibrium ph gel electrophoresis (nephge) / M.F. Lopez -- High-resolution, 2-D protein electrophoresis using nondedicated equipment / M. Sarmiento -- Large-gel 2-D electrophoresis / J. Klose -- Advantages of immobilized ph gradients / J. Fichmann -- Casting immobilized ph gradients (ipgs) / E. Gianazza -- Analytical ipg-dalt / A. Gorg and W. Weiss -- Ipg-dalt of very alkaline proteins / A. Gorg -- Running preparative carrier ampholyte and immobilized ph gradient ief gels for 2-D / N.M. Matsui [and others] -- In-gel sample rehydration of immobilized ph gradient / J.C. Sanchez, D. Hochstrasser and T. Rabilloud -- High-resolution, ipg-based, mini two-dimensional gel electrophoresis / J.C. Sanchez and D.F. Hochstrasser -- Horizontal sds-page for ipg-dalt / A. Gorg and W. Weiss -- Casting and running vertical slab-gel electrophoresis for 2D-page / B.J. Walsh and B.R. Herbert -- Nonreducing 2-D polyacrylamide gel electrophoresis / H. Ji and R.J. Simpson -- 2-D diagonal gel electrophoresis / J. Goverman -- 2-D phosphopeptide mapping / H. Nagahara [and others] -- Internal standards for 2-D / A.J. Link -- Autoradiography of 2-D gels / A.J. Link -- Double-label analysis / K.H. Lee and M.G. Harrington -- Silver staining of 2-D electrophoresis gels / T. Rabilloud -- Staining of preparative 2-D gels. Coomassie blue and imidazole-zinc negative staining / N.M. Matsui, D.M. Smith-Beckerman and L.B. Epstein -- Electroblotting of proteins from 2-D polyacrylamide gels / M.J. Dunn -- Detection of total proteins on western blots of 2-D polyacrylamide gels / M.J. Dunn -- Protein detection using reversible metal chelate stains / W.F. Patton, M.J. Lim and D. Shepro -- Glycoprotein detection of 2-D separated proteins / N.H. Packer, M.S. Ball and P.L. Devine -- Image acquisition in 2-D electrophoresis / W.F. Patton, M.J. Lim and D. Shepro -- Computer analysis of 2-D images / R.D. Appel and D.F. Hochstrasser -- 2-D databases on the World Wide Web / R.D. Appel, A. Bairoch and D.F. Hochstrasser -- Comparing 2-D electrophoretic gels across internet databases / P.F. Lemkin -- Constructing a 2-D database for the World Wide Web / R.D. Appel [and others] -- Absolute quantitation of 2-D protein spots / S.P. Gygi and R. Aebersold -- Generating a bacterial genome inventory. Identifying 2-D spots by comigrating products of the genome on 2-D gels / R.A. VanBogelen -- Immunoaffinity identification of 2-De separated proteins / B. Magi [and others] -- 2-De spot amino acid analysis with 9-fluorenylmethyl chloroformate / M.R. Wilkins, J.X. Yan and A.A. Gooley -- N-terminal amino acid sequencing of 2-De spots / M. Kamo and A. Tsugita -- Characterizing proteins from 2-De gels by internal sequence analysis of peptide fragments. Strategies for microsample handling / H. Erdjument-Bromage [and others] -- Obtaining molecular weights of proteins and their cleavage products by directly combining gel electrophoresis with mass spectrometry / R.R. Ogorzalek Loo, J.A. Loo and P.C. Andrews -- Identification of proteins by matrix-assisted laser desorption/ionization mass spectrometry using peptide and fragment ion masses / P.L. Courchesne and S.D. Patterson -- Sample preparation methods for mass spectrometric peptide mapping directly from 2-De gels / O.N. Jensen [and others] -- Protein identification and analysis tools in the expasy server / M.R. Wilkins [and others] -- Automated protein identification using microcolumn liquid chromatography-tandem mass spectrometry / J.R. Yates, 3rd [and others] -- Peptide sequencing of 2-De gel-isolated proteins by nanoelectrospray tandem mass spectrometry / O.N. Jensen [and others]
Control code
170925862
Dimensions
unknown
Extent
1 online resource (xvii, 601 pages)
File format
unknown
Form of item
online
Isbn
9781592595846
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592595847
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)170925862
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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