The Resource A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza, by Leonard L. Molotsky

A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza, by Leonard L. Molotsky

Label
A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza
Title
A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza
Statement of responsibility
by Leonard L. Molotsky
Title variation
Study of the absorption and assimilation of tryptophan two C fourteen by Spirodela polyrhiza
Creator
Contributor
Thesis advisor
Subject
Language
eng
Summary
The determination of tryptophan-2 C14 absorption and assimilation by Spirodela polyrhiza, grown in sterile inorganic nutrient solutions, the same nutrients in which these plants have satisfactorily carried on their life functions, was investigated by the use of descenting paper chromatography with results obtained by both the development of the chromatographs for visual inspection of tryptophan presence and by the detection of radioactivity from these chromatographed strips. Tryptophan absorption was also evidenced by radioactive determination per weight of plant tissue at various intervals of time the plants had been growing in tryptophan-2 C14 incorporated media. In addition to the plant tissue chromatographs, the nutrient solutions in which the plants had been growing were chromatographed and examined. All plants had been growing in the tryptophan-2 C14 incorporated solution and each flask of these plants was subjected to investigation, then a new flask of plants was investigated at the next predetermined time. This was continued until all the flasks of plants and their solutions were examined for the following periods in which the plants were growing and reproducing in the tryptophan-2 C14 media; 1, 2, 3, 4, 5, 7, 9, and 11 days. Plants had been grown under 15 hours of artificial lights per 24 hour periods the intensity of the light varied with the reference position under the lights from 320 to 520 foot candles. Average uniform illumination was maintained by using a turn table. The temperature was 30° C with lights operating and dropped to 17° C in darkness. The results of these experiments indicated that Spirodela polyhiza does absorb and assimilate tryptophan when it is incorporated into an inorganic media. The radioactivity of the plant tissue from various flasks examined indicates tryptophan is absorbed slowly at first and reaches a maximum concentration in the fourth and fifth day. After this period, the radioactivity decreases with increase in growing time. However the chromatographs of the hydrolyzed plant material indicates two radioactive zones, thus giving evidence of tryptophan assimilation after its absorption. The second zone was believed to be indoleacetic acid. The graphs made from the radiation of these spots indicated a maximum concentration of activity on the fourth and fifth day; after this period, the radioactivity decreased. An investigation of the plant nutrient solutions chromatographically also gave evidence of tryptophan absorption by the plants. There was observed from the solutions, the same substance as was found in the plant tissues (determined by Rf value). This indicated diffusion or perhaps that the plants assimilate tryptophan and produce the substance, believed to be indoleacetic acid, faster than it is utilized by plants. On the eleventh day, there was a complete absence of tryptophan in the media, this depletion of tryptophan in the solutions might answer the questions arising from a lowered actifvity in plants with increase in growing time. A mysterious third zone of activity appeared in the chromatographs of the solutions and increased in concentration with growing time. Nowhere in the literature could this zone be identified. Its Rf value indicates a molecular linkage
Cataloging source
UMK
http://library.link/vocab/creatorName
Molotsky, Leonard L
Degree
M.A.
Dissertation year
1959.
Granting institution
Dept. of Biology. University of Kansas City
Illustrations
  • illustrations
  • plates
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1895-
http://library.link/vocab/relatedWorkOrContributorName
Saeger, Albert Carl Julian
http://library.link/vocab/subjectName
Plants
Label
A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza, by Leonard L. Molotsky
Instantiates
Publication
Note
  • Advisor: Albert Saeger
  • Typescript
Bibliography note
Includes bibliographical references (leaves 98-101)
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction. Introduction ; History and literature -- Preliminary experimentation. The purification of Spirodela polyrhiza ; Tryptophan chromatography and detection ; Toxicity of tryptophan to Spirodela polyrhiza ; Nutrient solutions for tryptophan incorporation as a nutrient for Spirodela polyrhiza ; Aseptic media replacement procedures ; Controlled lighting for Spirodela polyrhiza metabolism ; Radiation detector manipulations -- Experiments with Spirodela polyrhiza growing in the presence of labeled tryptophan. Experiment I- Tryptophan presence in Spirodela polyrhiza grown only in S/5 solution ; Experiment II- Tryptophan2 C14 Absorption and assimilation by Spirodela polyrhiza ; Experiment III- Radioactive chromatography studies of the nutrient solutions of sirodela polyrhiza -- Discussion and summary -- Plates -- Bibliography
Control code
33167301
Dimensions
28 cm
Extent
vi, 101 leaves
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(OCoLC)33167301
Label
A study of the absorption and assimilation of tryptophan-2 C14 by Spirodela polyrhiza, by Leonard L. Molotsky
Publication
Note
  • Advisor: Albert Saeger
  • Typescript
Bibliography note
Includes bibliographical references (leaves 98-101)
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction. Introduction ; History and literature -- Preliminary experimentation. The purification of Spirodela polyrhiza ; Tryptophan chromatography and detection ; Toxicity of tryptophan to Spirodela polyrhiza ; Nutrient solutions for tryptophan incorporation as a nutrient for Spirodela polyrhiza ; Aseptic media replacement procedures ; Controlled lighting for Spirodela polyrhiza metabolism ; Radiation detector manipulations -- Experiments with Spirodela polyrhiza growing in the presence of labeled tryptophan. Experiment I- Tryptophan presence in Spirodela polyrhiza grown only in S/5 solution ; Experiment II- Tryptophan2 C14 Absorption and assimilation by Spirodela polyrhiza ; Experiment III- Radioactive chromatography studies of the nutrient solutions of sirodela polyrhiza -- Discussion and summary -- Plates -- Bibliography
Control code
33167301
Dimensions
28 cm
Extent
vi, 101 leaves
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(OCoLC)33167301

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