The Resource Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2, by Jacob Hicks, (electronic resource)

Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2, by Jacob Hicks, (electronic resource)

Label
Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2
Title
Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2
Statement of responsibility
by Jacob Hicks
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Oligomeric amyloid-[beta] peptide (A[beta]) is known to induce cytotoxic effects and damage cell functions in Alzheimer's disease. However, mechanisms underlying the effects of A[beta] on cell membranes have yet to be fully elucidated. In this study, A[beta] 1-42 (A[beta]42) was shown to cause a temporal biphasic change in membranes of astrocytic DITNC cells using fluorescence microscopy of Laurdan. A[beta]42 made astrocyte cell membranes become more molecularly-disordered after 30 minutes to 1 hour, transitioning to more molecularly-ordered after 3 hours. However, A[beta]42 caused artificial vesicle membranes made of rat whole brain lipid extract to become more disordered only. The trend for more molecularly-ordered membranes in astrocytes was abrogated by either an NADPH oxidase inhibitor, apocynin, or an inhibitor of cytosolic phospholipase A2 (cPLA2), but not by an inhibitor of calcium-independent PLA2 (iPLA2). Apocynin also suppressed the increased production of superoxide anions (O2-) and phosphorylation of cPLA2 induced by A[beta]42 . In addition, hydrolyzed products of cPLA2, arachidonic acid (AA), but not lysophosphatidylcholine (LPC) caused astrocyte membranes to become more molecularly-ordered. These results suggest (1) a direct interaction of A[beta]42 with cell membranes making them more molecularly-disordered, and (2) A[beta]42 indirectly makes membranes become more molecularly-ordered by triggering the signaling pathway involving NADPH oxidase and cPLA2
Cataloging source
MUU
http://library.link/vocab/creatorName
Hicks, Jacob
Degree
M.S.
Dissertation year
2010.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Lee, James C-M
http://library.link/vocab/subjectName
  • Phospholipase A2
  • Alzheimer's disease
  • Amyloid beta-protein
Target audience
specialized
Label
Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2, by Jacob Hicks, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on June 14, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. James Lee
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
648195908
Extent
1 online resource (vi, 38 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)648195908
Label
Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A2, by Jacob Hicks, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on June 14, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. James Lee
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
648195908
Extent
1 online resource (vi, 38 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)648195908

Library Locations

  • Ellis LibraryBorrow it
    1020 Lowry Street, Columbia, MO, 65201, US
    38.944491 -92.326012
  • Engineering Library & Technology CommonsBorrow it
    W2001 Lafferre Hall, Columbia, MO, 65211, US
    38.946102 -92.330125
  • Fisher Delta Research CenterBorrow it
    2-64 Agricultural Bldg, Columbia, MO, 65201, US
    38.958397 -92.303491
  • Geological Sciences LibraryBorrow it
    201 Geological Sciences, Columbia, MO, 65211, US
    38.947375 -92.329062
  • J. Otto Lottes Health Sciences LibraryBorrow it
    1 Hospital Dr, Columbia, MO, 65201, US
    38.939544 -92.328377
  • Journalism LibraryBorrow it
    102 Reynolds Jrnlism Institute, Columbia, MO, 65211, US
    38.947290 -92.328025
  • Mathematical Sciences LibraryBorrow it
    104 Ellis Library, Columbia, MO, 65201, US
    38.944377 -92.326537
  • University ArchivesBorrow it
    Columbia, MO, 65201, US
  • University Archives McAlester AnnexBorrow it
    703 Lewis Hall, Columbia, MO, 65211, US
    38.934630 -92.342290
  • University of Missouri Libraries DepositoryBorrow it
    2908 Lemone Blvd, Columbia, MO, 65211, US
    38.919360 -92.291620
  • Zalk Veterinary Medical LibraryBorrow it
    Veterinary Medicine West, Columbia, MO, 65211, US
    38.941099 -92.317911
Processing Feedback ...