The Resource An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates, by Bhanu Prakash V. L. Telugu, (electronic resource)

An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates, by Bhanu Prakash V. L. Telugu, (electronic resource)

Label
An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates
Title
An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates
Statement of responsibility
by Bhanu Prakash V. L. Telugu
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
The Pregnancy Associated Glycoproteins (PAGs) represent a multigene family of trophoblast expressed proteins, found exclusively in the placenta of even-toed mammals such as ruminants (cattle, sheep), pig, etc. In ruminants, the PAGs can be classified into ancient and modern PAGs based on their coding sequence. In addition, there are also differences in purported enzymatic activity as well as transcriptional regulation of expression. Many of the modern PAGs have accumulated mutations in and around the catalytic center, and some of those that incurred mutations in the two catalytic aspartates are predicted to be proteolytically inactive. In contrast, most of the ancient PAGs of ruminants and swine, have all the hallmarks of typical aspartic peptidases (APs). From the analysis of cattle genome, we found that there are 18 distinct PAG genes and 14 pseudogenes. Based on our preliminary analysis of the proximal promoter regions [500 base pairs (bp) upstream of the translational start point] of PAG genes, we found that there are pockets of conserved transcription factor binding sites that are different between ancient and modern PAGs. These differences likely influence the observed differences in expression between ancient and modern boPAGs. We gathered evidence by Real-time PCR and global analysis of expressed ESTs that confirm that, boPAG-2 is the most abundant of all boPAGs. We identified boPAG-2 and its closest paralog boPAG-12, as well as poPAG-2 the ancient PAG found in pigs, as the candidates for investigation of proteolytic activity. From our experiments we found that, boPAGs -2 and -12 and poPAG-2 are proteases with optimal activity under acidic pH conditions. We also illustrated differences in proteolytic activity towards substrates, and in their relative affinity towards an AP inhibitor (pepstatin A). We found that, in comparison to the two bovine paralogs, boPAGs -2 and -12, poPAG-2 was found to be a more robust enzyme. Finally, we demonstrated that APs secreted by embryos such as PAGs can be objectively measured in the medium conditioned by the culture of porcine embryos either individually or in pools for variable lengths of time in acidic conditions. We also observed that such activity seemed to correlate with stage and quality of embryos (assessed morphologically) in vitro. We, therefore, believe that this proteolytic activity potentially could serve as a marker for developmental competence of the embryos
Cataloging source
MUU
http://library.link/vocab/creatorDate
1977-
http://library.link/vocab/creatorName
Telugu, Bhanu Prakash V. L.
Degree
Ph.D.
Dissertation year
2008.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1969-
http://library.link/vocab/relatedWorkOrContributorName
Green, Jonathan A.
http://library.link/vocab/subjectName
  • Artiodactyla
  • Aspartic acid
  • Glycoproteins
  • Peptidase
  • Microbial mutation
  • Trophoblast
Target audience
specialized
Label
An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates, by Bhanu Prakash V. L. Telugu, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on February 23, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Jonathan A. Green
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
610015610
Extent
1 online resource (xviii, 247 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)610015610
Label
An analysis of aspartic peptidases expressed by trophoblasts and placenta of even-toed ungulates, by Bhanu Prakash V. L. Telugu, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on February 23, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Jonathan A. Green
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
610015610
Extent
1 online resource (xviii, 247 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)610015610

Library Locations

  • Ellis LibraryBorrow it
    1020 Lowry Street, Columbia, MO, 65201, US
    38.944491 -92.326012
  • Engineering Library & Technology CommonsBorrow it
    W2001 Lafferre Hall, Columbia, MO, 65211, US
    38.946102 -92.330125
  • Fisher Delta Research CenterBorrow it
    2-64 Agricultural Bldg, Columbia, MO, 65201, US
    38.958397 -92.303491
  • Geological Sciences LibraryBorrow it
    201 Geological Sciences, Columbia, MO, 65211, US
    38.947375 -92.329062
  • J. Otto Lottes Health Sciences LibraryBorrow it
    1 Hospital Dr, Columbia, MO, 65201, US
    38.939544 -92.328377
  • Journalism LibraryBorrow it
    102 Reynolds Jrnlism Institute, Columbia, MO, 65211, US
    38.947290 -92.328025
  • Mathematical Sciences LibraryBorrow it
    104 Ellis Library, Columbia, MO, 65201, US
    38.944377 -92.326537
  • University ArchivesBorrow it
    Columbia, MO, 65201, US
  • University Archives McAlester AnnexBorrow it
    703 Lewis Hall, Columbia, MO, 65211, US
    38.934630 -92.342290
  • University of Missouri Libraries DepositoryBorrow it
    2908 Lemone Blvd, Columbia, MO, 65211, US
    38.919360 -92.291620
  • Zalk Veterinary Medical LibraryBorrow it
    Veterinary Medicine West, Columbia, MO, 65211, US
    38.941099 -92.317911
Processing Feedback ...