The Resource Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques, by Wei Chen

Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques, by Wei Chen

Label
Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques
Title
Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques
Statement of responsibility
by Wei Chen
Title variation
Transport properties of plant cells
Creator
Subject
Language
eng
Summary
Confocal laser scanning microscopy (CLSM) and 3-D reconstruction methods were utilized to measure plant cell volume and surface area non-invasively. Carrot cells ( Daucus Carota L .) from suspension were used as model cells in this study. CLSM allowed observation and optical sectioning of cells in real time and three-dimensional imaging of the cells. The non-physical sectioning of cells permitted visualization of cellular changes in a natural state with reduced artifacts. Images acquired from CLSM were reconstructed into 3-D objects for visualization and geometric measurement. The volume and surface area of individual plant cells were obtained from the reconstructed 3-D images. CLSM and 3-D reconstruction offered much more consistent measurements than manual estimation. The geometric measurements from CLSM and 3-D reconstruction were significantly different from conventional 2-D estimations. The techniques developed in this study were validated by processing images of calibration micro-spheres of known size and shape. A micro-diffusion chamber system was developed for measuring water permeability of plant cells. The chamber was mounted on an inverted microscope. Direct observation of dynamic osmotic response of plant cells was made with a digital camera and video-cassette-recorder (VCR) system. Suspension tobacco cells ( Nicotiana tabacum L . "Hicks") were used as model specimens. After equilibration in 7% (w/v) NaCl solution (229mOsm) for 30 minutes, the cells were exposed to 3% NaCl solution (962mOsm) in the micro-diffusion chamber. The 3% NaCl solution was supplied at a rate of 35 ml/min at room temperature (about 25°C). Cell size changes were videotaped and analyzed with a parameter estimation program. Water permeability of cell membrane was determined from the cell size measurement. For tobacco cells, the membrane water permeability was 0.26 ± 0.03 om/min·atm. The osmotically inactive fraction of the cell volume ( V s ) was obtained by using the Boyle-Van't Hoff plot. It was about 25% of isotonic cell volume. The effects of freezing and blanching were analyzed with a temperature-control-stage. Different processing procedures did not cause significant changes in the mean value of water permeability. This result is in agreement with previous reports on animal and human cells. It suggests that osmotic behavior of cell membrane (expansion or shrinkage) is not an elastic and reversible process. It also suggests that, in terms of osmotic properties, control tobacco cell membranes can be used as a reasonable approximation for membranes of frozen-thawed, blanched, and blanched-frozen-thawed cells
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1963-
http://library.link/vocab/creatorName
Chen, Wei
Degree
Ph. D.
Dissertation year
1999.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
bibliography
http://library.link/vocab/subjectName
  • Plant cells and tissues
  • Confocal microscopy
  • Frozen foods
  • Food
Target audience
specialized
Label
Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques, by Wei Chen
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 99-102)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
46886398
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 125 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote
Label
Analysis of mass transport properties of plant cells by confocal microscopy and imaging techniques, by Wei Chen
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 99-102)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
46886398
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 125 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote

Library Locations

    • Engineering Library & Technology CommonsBorrow it
      W2001 Lafferre Hall, Columbia, MO, 65211, US
      38.946102 -92.330125
    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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