The Resource Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum, by Sung-Lim Yu

Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum, by Sung-Lim Yu

Label
Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum
Title
Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum
Statement of responsibility
by Sung-Lim Yu
Title variation
NER gene response to DNA damage in Dictyostelium
Creator
Subject
Language
eng
Summary
Nucleotide excision repair (NER) is a complex multistep pathway in which many enzymes are involved. NER is the main damaged DNA repair system which repairs a variety of bulky lesions in both procaryotes and eucaryotes. Mutations in NER related genes in humans cause rare recessive genetic disorders such as xeroderma pigmentosum (XP). There are eight groups of XP and the cognate genes for each group have been cloned and characterized. However, the function of XPE gene is not completely understood. We have cloned and characterized the repE gene, the Dictyostelium homolog of XPE. The predicted RepE protein shares 40% identity and over 60% similarity with human XPE/UV-DDB protein and has a putative leucine zipper motif. repE mRNA is expressed at a low level in vegetatively growing cells and at the onset of development. However, the repE mRNA level increases to a maximum at 1-3 hours of development and then returns to a low baseline level for the remainder of development. The developmental expression pattern and the putative leucine zipper motif indicate that the RepE protein has a function during development. In order to explore the function of RepE, we attempted to clone the RepE partner using an interaction cloning method. The results were negative and the possible reasons are discussed. In addition to repE, we have cloned the repB and D genes, the Dictyostelium XPB and XPD homologs, respectively (Lee et al., 1997a; Lee, 1997). We have examined the response of the Dictyostelium NER genes to two DNA damaging agents, cisplatin and UV irradiation. Following cisplatin treatment, the cells showed a dose dependant killing rate, but they were unusually resistant to the drug compared to mammalian cells. A large fraction of cells still survived even after treatment with 330 $\mu$M of cisplatin for 24 hours. This high resistance appears to be due to a transient induction of some protection mechanisms rather than the selection of the resistant mutants. Dictyostelium cells are also more resistant to UV than other organisms. We examined the UV inducibility of the rep genes after UV irradiation. The repB and D genes are transiently induced in dose-dependant manner, while the repE gene expression was repressed. The repB gene induction is the first case of the induction of a XPB homolog by UV. This immediate and robust induction of the repB and D genes are possibly contributing factors of the high resistance of Dictyostelium cells to UV
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1965-
http://library.link/vocab/creatorName
Yu, Sung-Lim
Degree
Ph. D.
Dissertation year
1997.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Dictyostelium discoideum
  • Molecular genetics
  • Nucleotides
  • Plant genetics
  • DNA repair
  • DNA replication
  • Genetic transcription
  • Genetic recombination
  • Introns
  • Cisplatin
  • Ultraviolet radiation
Target audience
specialized
Label
Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum, by Sung-Lim Yu
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 109-130)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
42585920
Dimensions
29 cm
Dimensions
unknown
Extent
xiii, 131 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
Label
Analysis of the response of nucleotide excision repair genes in Dictyostelium discoideum, by Sung-Lim Yu
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 109-130)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
42585920
Dimensions
29 cm
Dimensions
unknown
Extent
xiii, 131 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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