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The Resource Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants, by Rhianna M. Wallace

Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants, by Rhianna M. Wallace

Label
Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants
Title
Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants
Statement of responsibility
by Rhianna M. Wallace
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
Pregnancy-associated glycoproteins (PAGs) belong to the aspartic protease family and are expressed exclusively by trophoblasts of even-toed ungulates. This study was designed to provide insight into some of the biological roles of bovine PAGs in ruminant pregnancy by measuring changes in transcript abundance in cultured endometrial explants from pregnant and non-pregnant heifers treated with PAGs. A mixture of PAG-4, -6, and -9 were purified from mid-gestation bovine placental extracts. Heifers were synchronized and bred by artificial insemination to either high fertility semen (n = 14) or dead semen (n = 6), then slaughtered at day 18 post-breeding. Endometrial explants were collected and split between 4 groups: pregnant + 15 [mu]g/ml PAG (n = 10), pregnant + no PAG (n = 10), non-pregnant + 15 [mu]g/ml PAG (n = 10), and non-pregnant + no PAG (n = 10). The transcript abundance for several tissue remodeling related target genes [extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinases (MMP) 1, 2, 3, 7, 8, and 9, plaminogen activator urokinase (PLAU), osteopontin (SPP1), tissue inhibitors of MMP 1 and 2 (TIMP1, TIMP2)], were analyzed in endometrial tissue by quantitative PCR. We observed consistent changes in transcript abundance between pregnancy groups for MMP1, 3, 7, 8, PLAU, SPP1 at 24 hours and MMP1, 3, 8, PLAU, SPP1 at 96 hours. These results indicate that the PAGs used in this experiment are capable of inducing changes in transcripts associated with matrix remodeling in bovine endometrial explants. This model system may be useful for assessing PAG function in the uterine stroma
Cataloging source
MUU
http://library.link/vocab/creatorName
Wallace, Rhianna M
Degree
M.S.
Dissertation note
Thesis
Dissertation year
2015.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1969-
http://library.link/vocab/relatedWorkOrContributorName
  • Green, Jonathan A.
  • Smith, Michael Fielding
http://library.link/vocab/subjectName
  • Glycoproteins
  • Ruminants
Label
Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants, by Rhianna M. Wallace
Instantiates
Publication
Note
Dr. Jonathan Green and Dr. Michael Smith, Thesis Supervisors
Bibliography note
Includes bibliographical references (pages 82-92)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
997475195
Extent
1 online resource (ix, 92 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
System control number
(OCoLC)997475195
Label
Analysis of transcript abundance of uterine genes after application of bovine pregnancy associated glycoproteins to endometrial explants, by Rhianna M. Wallace
Publication
Note
Dr. Jonathan Green and Dr. Michael Smith, Thesis Supervisors
Bibliography note
Includes bibliographical references (pages 82-92)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
997475195
Extent
1 online resource (ix, 92 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
System control number
(OCoLC)997475195

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