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The Resource Ancient DNA typing : methods, strategies and applications, Susanne Hummel

Ancient DNA typing : methods, strategies and applications, Susanne Hummel

Label
Ancient DNA typing : methods, strategies and applications
Title
Ancient DNA typing
Title remainder
methods, strategies and applications
Statement of responsibility
Susanne Hummel
Creator
Subject
Language
eng
Summary
"Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering."--Jacket
Cataloging source
DLC
http://library.link/vocab/creatorName
Hummel, Susanne
Dewey number
611/.01816
Illustrations
illustrations
Index
index present
LC call number
QP624
LC item number
.H866 2003
Literary form
non fiction
Nature of contents
bibliography
http://library.link/vocab/subjectName
  • DNA, Fossil
  • Mitochondrial DNA
  • Nucleotide sequence
  • Polymerase chain reaction
  • DNA
  • Fossils
  • Base Sequence
  • Polymerase Chain Reaction
Label
Ancient DNA typing : methods, strategies and applications, Susanne Hummel
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • X-chromosomal STRs
  • ABO blood group genes
  • CCR5
  • [Delta]F508
  • aDNA extraction
  • Comparison of extraction methods
  • aDNA yield
  • aDNA preservation
  • aDNA degradation patterns
  • The age of aDNA
  • DNA markers
  • Fragment lengths of aDNA
  • Storage of aDNA extracts
  • PCR
  • Basic PCR mechanism
  • Number of amplification cycles
  • Cycling parameters
  • Annealing temperature
  • Denaturation temperature
  • Elongation temperature
  • Durations of the cycling steps
  • Mitochondrial DNA
  • Primer design
  • Matching primers
  • Primer energy profile
  • Annealing temperature, primer dimer and hairpins
  • Mismatch primers
  • Software packages
  • Primer design--step by step
  • Multiplex PCR
  • PCR failures
  • DNA degradation
  • The hypervariable regions and control region V
  • Inhibition
  • DNA overload and overcycling
  • Analysis of PCR products
  • Fragment length analysis
  • Agarose gel electrophoresis
  • Automated fragment length determination
  • Sequence analysis
  • Direct sequencing vs. sequencing clones
  • Overlapping sequences vs. a multiplex approach
  • RFLP analysis
  • Cytochrome b
  • Authenticity of results
  • Contamination sources
  • Contamination through handling
  • Contamination in reaction tubes
  • Cleaning the PCR reaction tubes
  • PCR in glass tubes
  • Validating aDNA results
  • Ring exercises
  • Applications
  • Sex determination
  • Chromosomal DNA
  • The children of Aegerten
  • Sex determination by multiplex amplification
  • Identification
  • Amelogenin
  • Autosomal STRs
  • Y-chromosomal STRs
Control code
50422937
Dimensions
24 cm
Extent
xi, 298 pages
Isbn
9783540430377
Lccn
2002030385
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
Label
Ancient DNA typing : methods, strategies and applications, Susanne Hummel
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • X-chromosomal STRs
  • ABO blood group genes
  • CCR5
  • [Delta]F508
  • aDNA extraction
  • Comparison of extraction methods
  • aDNA yield
  • aDNA preservation
  • aDNA degradation patterns
  • The age of aDNA
  • DNA markers
  • Fragment lengths of aDNA
  • Storage of aDNA extracts
  • PCR
  • Basic PCR mechanism
  • Number of amplification cycles
  • Cycling parameters
  • Annealing temperature
  • Denaturation temperature
  • Elongation temperature
  • Durations of the cycling steps
  • Mitochondrial DNA
  • Primer design
  • Matching primers
  • Primer energy profile
  • Annealing temperature, primer dimer and hairpins
  • Mismatch primers
  • Software packages
  • Primer design--step by step
  • Multiplex PCR
  • PCR failures
  • DNA degradation
  • The hypervariable regions and control region V
  • Inhibition
  • DNA overload and overcycling
  • Analysis of PCR products
  • Fragment length analysis
  • Agarose gel electrophoresis
  • Automated fragment length determination
  • Sequence analysis
  • Direct sequencing vs. sequencing clones
  • Overlapping sequences vs. a multiplex approach
  • RFLP analysis
  • Cytochrome b
  • Authenticity of results
  • Contamination sources
  • Contamination through handling
  • Contamination in reaction tubes
  • Cleaning the PCR reaction tubes
  • PCR in glass tubes
  • Validating aDNA results
  • Ring exercises
  • Applications
  • Sex determination
  • Chromosomal DNA
  • The children of Aegerten
  • Sex determination by multiplex amplification
  • Identification
  • Amelogenin
  • Autosomal STRs
  • Y-chromosomal STRs
Control code
50422937
Dimensions
24 cm
Extent
xi, 298 pages
Isbn
9783540430377
Lccn
2002030385
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations

Library Locations

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      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
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