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The Resource Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics : a replacement for western blot analysis, Michael Kinter, Caroline S. Kinter

Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics : a replacement for western blot analysis, Michael Kinter, Caroline S. Kinter

Label
Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics : a replacement for western blot analysis
Title
Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics
Title remainder
a replacement for western blot analysis
Statement of responsibility
Michael Kinter, Caroline S. Kinter
Creator
Contributor
Author
Subject
Language
eng
Summary
A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins
Member of
Cataloging source
GW5XE
http://library.link/vocab/creatorName
Kinter, Michael
Dewey number
572/.6
Illustrations
illustrations
Index
no index present
LC call number
QP551
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 55
http://library.link/vocab/relatedWorkOrContributorName
Kinter, Caroline S.
Series statement
SpringerBriefs in Systems Biology,
http://library.link/vocab/subjectName
  • Proteomics
  • Proteins
  • Proteomics
  • Proteins
  • SCIENCE
  • Proteins
  • Proteomics
Label
Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics : a replacement for western blot analysis, Michael Kinter, Caroline S. Kinter
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Introduction to quantitative proteomics
  • Specificity of detection is the key attribute of selected reaction monitoring
  • Designing a selected reaction monitoring method
  • Sample processing
  • Sample analysis and data processing
  • Future directions
Control code
859665966
Dimensions
unknown
Extent
1 online resource (xiii, 65 pages)
File format
unknown
Form of item
online
Isbn
9781461486664
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4614-8666-4
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)859665966
Label
Application of selected reaction monitoring to highly multiplexed targeted quantitative proteomics : a replacement for western blot analysis, Michael Kinter, Caroline S. Kinter
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Introduction to quantitative proteomics
  • Specificity of detection is the key attribute of selected reaction monitoring
  • Designing a selected reaction monitoring method
  • Sample processing
  • Sample analysis and data processing
  • Future directions
Control code
859665966
Dimensions
unknown
Extent
1 online resource (xiii, 65 pages)
File format
unknown
Form of item
online
Isbn
9781461486664
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4614-8666-4
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)859665966

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      38.944491 -92.326012
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