The Resource Aptamer encoded nanopores as single molecule sensors, by Shu Ding, (electronic resource)

Aptamer encoded nanopores as single molecule sensors, by Shu Ding, (electronic resource)

Label
Aptamer encoded nanopores as single molecule sensors
Title
Aptamer encoded nanopores as single molecule sensors
Statement of responsibility
by Shu Ding
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
We have created a low noise, calibrated, molecular-scaled pore. The nanopore is formed either by micro-forge polishing or external penetration of the nanocavity sealed in the pipette terminal. The nanopore fabrication is both cost-effective and time-efficient. The uniform profile of the nanocavity is imaged by a scanning electron microscope (SEM). It suggested a molecular scale pore size and conical pore shape. By correlating pore size and conductance, we have established a method for calibrating pore size by conductance level. The pore diameter was further verified by the translocation of double-strand DNA (dsDNA), which has a known size 2ñm. Our study shows that glass nanopore can be modified with DNA/RNA aptamer as a promising biosensor. Single molecules (IgE, Ricin and streptavidin/biotin) were detected rapidly and sensitively in real time electrical measurement. Simultaneous recording showed a dynamic process in molecule recognition and interaction. This demonstrated that a glass nanopore could impact single protein molecule detection for medical and biothreat applications. One of the exciting observations is a single restriction enzyme (Hind III) activity within nano-confinement. The duration of event could indicate the strength of the interaction and the amplitude of the event could indicate different protein conformations. We demonstrated the ability of a functionalized nanopore to measure the recognition and interaction on a single molecule scale. It can provide deeper insight and understanding of the stochastic interaction of various biomolecules
Cataloging source
MUU
http://library.link/vocab/creatorName
Ding, Shu
Degree
M.S.
Dissertation year
2008.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Gu, Li-Qun
http://library.link/vocab/subjectName
  • Nanostructured materials
  • Biomolecules
  • Detectors
Target audience
specialized
Label
Aptamer encoded nanopores as single molecule sensors, by Shu Ding, (electronic resource)
Instantiates
Publication
Note
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on September 21, 2009)
  • Thesis advisor: Liqun Gu
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
437429245
Extent
1 online resource (x, 60 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)437429245
Label
Aptamer encoded nanopores as single molecule sensors, by Shu Ding, (electronic resource)
Publication
Note
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on September 21, 2009)
  • Thesis advisor: Liqun Gu
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
437429245
Extent
1 online resource (x, 60 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)437429245

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