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The Resource Arabidopsis : a practical approach, edited by Zoe A. Wilson

Arabidopsis : a practical approach, edited by Zoe A. Wilson

Label
Arabidopsis : a practical approach
Title
Arabidopsis
Title remainder
a practical approach
Statement of responsibility
edited by Zoe A. Wilson
Contributor
Subject
Genre
Language
eng
Summary
"Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--BOOK JACKET
Member of
Cataloging source
DLC
Dewey number
583/.64
Illustrations
illustrations
Index
index present
LC call number
QK495.C9
LC item number
A687 2000
Literary form
non fiction
NAL call number
QK495.C9
NAL item number
A687 2000
Nature of contents
bibliography
NLM call number
  • 2007 B-259
  • QK 495.C9
NLM item number
A6582 2000
http://library.link/vocab/relatedWorkOrContributorName
Wilson, Zoe A
Series statement
Practical approach series
Series volume
223
http://library.link/vocab/subjectName
  • Arabidopsis
  • Arabidopsis
Label
Arabidopsis : a practical approach, edited by Zoe A. Wilson
Instantiates
Publication
Note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • What makes Arabidopsis such an attractive experimental model?
  • Preparation and digestion of A. thaliana genomic DNA
  • 54
  • Preparation of genomic DNA
  • 56
  • Identifying an RFLP
  • 60
  • Southern blotting and hybridization
  • 61
  • 3.
  • Polymorphic markers
  • 3
  • 62
  • RFLP markers
  • 62
  • PCR-based markers
  • 66
  • Phenotypic and biochemical markers
  • 68
  • 4.
  • Calculating map positions
  • 69
  • 3.
  • Mapping programs
  • 69
  • NASC mapping service
  • 70
  • 5.
  • Integration of a mutation into a molecular map
  • 72
  • 4.
  • Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis
  • Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson
  • Arabidopsis genetic resource centres
  • 77
  • 1.
  • Generation of mutants and their importance for developmental biology
  • 77
  • 2.
  • Mapping and segregation analysis
  • 78
  • Mapping of mutations
  • 78
  • Influence of environment on phenotype
  • 4
  • 82
  • 3.
  • Microscopy
  • 82
  • Fresh material characterization
  • 82
  • Fixed material characterization
  • 84
  • 4.
  • Analysis of plant gene expression
  • Arabidopsis genetic resources
  • 89
  • RNA isolation
  • 89
  • Northern analysis
  • 91
  • In situ hybridizations
  • 92
  • 5.
  • Classical and molecular cytogenetics of Arabidopsis
  • G. H. Jones, J. S. Heslop-Harrison
  • 4
  • 105
  • 2.
  • Mitotic chromosome analysis by light microscopy
  • 108
  • 3.
  • In situ hybridization to mitotic chromosome preparations
  • 112
  • Photography of in situ hybridizations
  • 117
  • 4.
  • Accessing Arabidopsis resources
  • Meiotic chromosome analysis by light microscopy
  • 117
  • 5.
  • Meiotic chromosome analysis by electron microscopy
  • 121
  • 6.
  • Tissue culture, transformation, and transient gene expression in Arabidopsis
  • Keith Lindsey, Wenbin Wei
  • 125
  • 2.
  • 5
  • Stable transformation by Agrobacterium tumefaciens
  • 128
  • 3.
  • Transient gene expression in Arabidopsis protoplasts
  • 131
  • Reporter gene enzyme assays
  • 134
  • 7.
  • Transposon and T-DNA mutagenesis
  • Mark G. M. Aarts, Csaba Koncz, Andy Pereira
  • 4.
  • 143
  • 2.
  • Transposon tagging
  • 143
  • Endogenous transposable elements
  • 143
  • Transposon tagging systems in Arabidopsis
  • 144
  • Which system to use?
  • 149
  • 1.
  • Mutants of Arabidopsis
  • Genetic and molecular analysis of a putatively transposon tagged mutant
  • 151
  • Further applications of transposon tagging
  • 156
  • 3.2
  • T-DNA tagging
  • 158
  • Use of T-DNA as insertional mutagen
  • 158
  • Random tagging
  • 5
  • 158
  • Available populations of T-DNA transformants
  • 159
  • Promoter/enhancer trapping
  • 160
  • Analysis of T-DNA mutants and cloning a tagged gene
  • 161
  • Further applications of T-DNA tagging
  • 166
  • 8.
  • Single gene mutation lines
  • Map-based cloning in Arabidopsis
  • Joanna Putterill, George Coupland
  • 171
  • 2.
  • Locating the mutation of interest relative to DNA markers
  • 172
  • Determining an approximate map position
  • 172
  • Identifying a short genetic interval containing the mutation as a prelude to isolating the gene
  • 175
  • 5
  • 3.
  • Placing the gene on the physical map
  • 177
  • Chromosome landing
  • 177
  • Chromosome walking with YAC clones
  • 178
  • 4.
  • Identification of the gene
  • 189
  • Resources for the identification/investigation of novel genes
  • Location of the gene by molecular complementation
  • 189
  • Determining the structure of the gene
  • 194
  • 5.
  • Perspectives
  • 194
  • 9.
  • Physical mapping: YACs, BACs, cosmids, and nucleotide sequences
  • Ian Bancroft
  • 8
  • 199
  • 2.
  • Genome mapping with YAC clones
  • 199
  • 3.
  • Genome mapping with BAC and P1 clones
  • 202
  • Communal resources
  • 202
  • Construction of BAC libraries
  • Mapping tools
  • 208
  • Genome mapping with BACs
  • 211
  • 4.
  • High resolution mapping with cosmids
  • 216
  • Approaches to mapping with cosmids
  • 216
  • Construction of cosmid libraries
  • 216
  • 10
  • 5.
  • Nucleotide sequences--the ultimate mapping tool
  • 221
  • EST sequencing project
  • 221
  • Genome sequencing project
  • 221
  • Sequence-based mapping
  • 222
  • 10.
  • 5.
  • Web-based bioinformatic tools for Arabidopsis researchers
  • Seung Y. Rhee, David J. Flanders
  • 225
  • What is bioinformatics
  • 225
  • Sources of Arabidopsis bioinformatic data
  • 226
  • 2.
  • Basic tools for the Internet
  • 227
  • Considerations of available resources for identifying novel genes
  • Web basics
  • 227
  • Getting onto the Web
  • 228
  • Using your browser
  • 229
  • Browser tips and errors
  • 232
  • Privacy issues
  • 235
  • Growth, maintenance, and use of Arabidopsis genetic resources
  • 11
  • 3.
  • Scenarios of bioinformatic use in Arabidopsis research
  • 235
  • 4.
  • Gene information resources
  • 237
  • General gene information
  • 237
  • Arabidopsis gene information
  • 237
  • Forward genetics
  • Plant gene information
  • 238
  • Genetic maps
  • 239
  • Physical maps
  • 240
  • 6.
  • Sequencing
  • 242
  • Arabidopsis genome initiative (AGI)
  • 12
  • 243
  • Annotation of sequences by AGI
  • 243
  • Caveats in annotation
  • 244
  • Sequence contigs from AtDB
  • 245
  • 7.
  • Sequence analysis tools
  • 245
  • Reverse genetics
  • BLAST
  • 246
  • FASTA
  • 250
  • EST databases
  • 252
  • Gene identification programs
  • 253
  • Gene family analyses
  • 255
  • 14
  • Motif analyses
  • 255
  • Protein structures
  • 258
  • Comprehensive sequence analysis tools
  • 259
  • 8.
  • Current issues and future directions in bioinformatics
  • 260
  • Some important bioinformatic issues
  • 6.
  • 260
  • Bioinformatic tools currently under development for Arabidopsis research
  • Growing Arabidopsis
  • 15
  • How to maintain clean growth conditions
  • 15
  • Mary Anderson, Fiona Wilson
  • Growing Arabidopsis in the glasshouse
  • 16
  • Chemical control of pests and diseases
  • 19
  • 7.
  • Seed storage
  • 21
  • 8.
  • Growing Arabidopsis with specific growth requirements
  • 23
  • 1
  • 9.
  • Sterile culture of Arabidopsis
  • 24
  • 2.
  • Preservation and handling of stock centre clones
  • Randy Scholl, Keith Davis, Doreen Ware
  • 29
  • 2.
  • Missions of a plant DNA resource centre
  • 29
  • 1.
  • 3.
  • Preservation of stocks
  • 30
  • Plasmids with small DNA inserts
  • 30
  • Cosmids
  • 32
  • Phage and phage libraries
  • 33
  • Yeast artificial chromosome (YAC) libraries
  • What is Arabidopsis?
  • 33
  • Pools of YAC library cells for PCR screening
  • 37
  • Distribution of YAC libraries arrayed on nylon filters
  • 38
  • Other large-insert libraries
  • 38
  • Yeast expression analysis--'two-hybrid' libraries and complementation testing
  • 42
  • 4.
  • 1
  • Verification of stock identity and purity
  • 42
  • 5.
  • Pooled DNA from T-DNA lines for PCR screening
  • 44
  • 6.
  • Organization of stock information
  • 48
  • Collecting, maintaining, and disseminating stock data
  • 48
  • 2.
  • Organizing and distributing patron data
  • 48
  • 7.
  • Future
  • 48
  • 3.
  • Genetic mapping using recombinant inbred lines
  • Clare Lister, Mary Anderson, Caroline Dean
  • 51
  • 2.
Control code
42295871
Dimensions
24 cm
Extent
xix, 275 pages
Isbn
9780199635658
Lccn
99045851
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(OCoLC)42295871
Label
Arabidopsis : a practical approach, edited by Zoe A. Wilson
Publication
Note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • What makes Arabidopsis such an attractive experimental model?
  • Preparation and digestion of A. thaliana genomic DNA
  • 54
  • Preparation of genomic DNA
  • 56
  • Identifying an RFLP
  • 60
  • Southern blotting and hybridization
  • 61
  • 3.
  • Polymorphic markers
  • 3
  • 62
  • RFLP markers
  • 62
  • PCR-based markers
  • 66
  • Phenotypic and biochemical markers
  • 68
  • 4.
  • Calculating map positions
  • 69
  • 3.
  • Mapping programs
  • 69
  • NASC mapping service
  • 70
  • 5.
  • Integration of a mutation into a molecular map
  • 72
  • 4.
  • Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis
  • Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson
  • Arabidopsis genetic resource centres
  • 77
  • 1.
  • Generation of mutants and their importance for developmental biology
  • 77
  • 2.
  • Mapping and segregation analysis
  • 78
  • Mapping of mutations
  • 78
  • Influence of environment on phenotype
  • 4
  • 82
  • 3.
  • Microscopy
  • 82
  • Fresh material characterization
  • 82
  • Fixed material characterization
  • 84
  • 4.
  • Analysis of plant gene expression
  • Arabidopsis genetic resources
  • 89
  • RNA isolation
  • 89
  • Northern analysis
  • 91
  • In situ hybridizations
  • 92
  • 5.
  • Classical and molecular cytogenetics of Arabidopsis
  • G. H. Jones, J. S. Heslop-Harrison
  • 4
  • 105
  • 2.
  • Mitotic chromosome analysis by light microscopy
  • 108
  • 3.
  • In situ hybridization to mitotic chromosome preparations
  • 112
  • Photography of in situ hybridizations
  • 117
  • 4.
  • Accessing Arabidopsis resources
  • Meiotic chromosome analysis by light microscopy
  • 117
  • 5.
  • Meiotic chromosome analysis by electron microscopy
  • 121
  • 6.
  • Tissue culture, transformation, and transient gene expression in Arabidopsis
  • Keith Lindsey, Wenbin Wei
  • 125
  • 2.
  • 5
  • Stable transformation by Agrobacterium tumefaciens
  • 128
  • 3.
  • Transient gene expression in Arabidopsis protoplasts
  • 131
  • Reporter gene enzyme assays
  • 134
  • 7.
  • Transposon and T-DNA mutagenesis
  • Mark G. M. Aarts, Csaba Koncz, Andy Pereira
  • 4.
  • 143
  • 2.
  • Transposon tagging
  • 143
  • Endogenous transposable elements
  • 143
  • Transposon tagging systems in Arabidopsis
  • 144
  • Which system to use?
  • 149
  • 1.
  • Mutants of Arabidopsis
  • Genetic and molecular analysis of a putatively transposon tagged mutant
  • 151
  • Further applications of transposon tagging
  • 156
  • 3.2
  • T-DNA tagging
  • 158
  • Use of T-DNA as insertional mutagen
  • 158
  • Random tagging
  • 5
  • 158
  • Available populations of T-DNA transformants
  • 159
  • Promoter/enhancer trapping
  • 160
  • Analysis of T-DNA mutants and cloning a tagged gene
  • 161
  • Further applications of T-DNA tagging
  • 166
  • 8.
  • Single gene mutation lines
  • Map-based cloning in Arabidopsis
  • Joanna Putterill, George Coupland
  • 171
  • 2.
  • Locating the mutation of interest relative to DNA markers
  • 172
  • Determining an approximate map position
  • 172
  • Identifying a short genetic interval containing the mutation as a prelude to isolating the gene
  • 175
  • 5
  • 3.
  • Placing the gene on the physical map
  • 177
  • Chromosome landing
  • 177
  • Chromosome walking with YAC clones
  • 178
  • 4.
  • Identification of the gene
  • 189
  • Resources for the identification/investigation of novel genes
  • Location of the gene by molecular complementation
  • 189
  • Determining the structure of the gene
  • 194
  • 5.
  • Perspectives
  • 194
  • 9.
  • Physical mapping: YACs, BACs, cosmids, and nucleotide sequences
  • Ian Bancroft
  • 8
  • 199
  • 2.
  • Genome mapping with YAC clones
  • 199
  • 3.
  • Genome mapping with BAC and P1 clones
  • 202
  • Communal resources
  • 202
  • Construction of BAC libraries
  • Mapping tools
  • 208
  • Genome mapping with BACs
  • 211
  • 4.
  • High resolution mapping with cosmids
  • 216
  • Approaches to mapping with cosmids
  • 216
  • Construction of cosmid libraries
  • 216
  • 10
  • 5.
  • Nucleotide sequences--the ultimate mapping tool
  • 221
  • EST sequencing project
  • 221
  • Genome sequencing project
  • 221
  • Sequence-based mapping
  • 222
  • 10.
  • 5.
  • Web-based bioinformatic tools for Arabidopsis researchers
  • Seung Y. Rhee, David J. Flanders
  • 225
  • What is bioinformatics
  • 225
  • Sources of Arabidopsis bioinformatic data
  • 226
  • 2.
  • Basic tools for the Internet
  • 227
  • Considerations of available resources for identifying novel genes
  • Web basics
  • 227
  • Getting onto the Web
  • 228
  • Using your browser
  • 229
  • Browser tips and errors
  • 232
  • Privacy issues
  • 235
  • Growth, maintenance, and use of Arabidopsis genetic resources
  • 11
  • 3.
  • Scenarios of bioinformatic use in Arabidopsis research
  • 235
  • 4.
  • Gene information resources
  • 237
  • General gene information
  • 237
  • Arabidopsis gene information
  • 237
  • Forward genetics
  • Plant gene information
  • 238
  • Genetic maps
  • 239
  • Physical maps
  • 240
  • 6.
  • Sequencing
  • 242
  • Arabidopsis genome initiative (AGI)
  • 12
  • 243
  • Annotation of sequences by AGI
  • 243
  • Caveats in annotation
  • 244
  • Sequence contigs from AtDB
  • 245
  • 7.
  • Sequence analysis tools
  • 245
  • Reverse genetics
  • BLAST
  • 246
  • FASTA
  • 250
  • EST databases
  • 252
  • Gene identification programs
  • 253
  • Gene family analyses
  • 255
  • 14
  • Motif analyses
  • 255
  • Protein structures
  • 258
  • Comprehensive sequence analysis tools
  • 259
  • 8.
  • Current issues and future directions in bioinformatics
  • 260
  • Some important bioinformatic issues
  • 6.
  • 260
  • Bioinformatic tools currently under development for Arabidopsis research
  • Growing Arabidopsis
  • 15
  • How to maintain clean growth conditions
  • 15
  • Mary Anderson, Fiona Wilson
  • Growing Arabidopsis in the glasshouse
  • 16
  • Chemical control of pests and diseases
  • 19
  • 7.
  • Seed storage
  • 21
  • 8.
  • Growing Arabidopsis with specific growth requirements
  • 23
  • 1
  • 9.
  • Sterile culture of Arabidopsis
  • 24
  • 2.
  • Preservation and handling of stock centre clones
  • Randy Scholl, Keith Davis, Doreen Ware
  • 29
  • 2.
  • Missions of a plant DNA resource centre
  • 29
  • 1.
  • 3.
  • Preservation of stocks
  • 30
  • Plasmids with small DNA inserts
  • 30
  • Cosmids
  • 32
  • Phage and phage libraries
  • 33
  • Yeast artificial chromosome (YAC) libraries
  • What is Arabidopsis?
  • 33
  • Pools of YAC library cells for PCR screening
  • 37
  • Distribution of YAC libraries arrayed on nylon filters
  • 38
  • Other large-insert libraries
  • 38
  • Yeast expression analysis--'two-hybrid' libraries and complementation testing
  • 42
  • 4.
  • 1
  • Verification of stock identity and purity
  • 42
  • 5.
  • Pooled DNA from T-DNA lines for PCR screening
  • 44
  • 6.
  • Organization of stock information
  • 48
  • Collecting, maintaining, and disseminating stock data
  • 48
  • 2.
  • Organizing and distributing patron data
  • 48
  • 7.
  • Future
  • 48
  • 3.
  • Genetic mapping using recombinant inbred lines
  • Clare Lister, Mary Anderson, Caroline Dean
  • 51
  • 2.
Control code
42295871
Dimensions
24 cm
Extent
xix, 275 pages
Isbn
9780199635658
Lccn
99045851
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(OCoLC)42295871

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      38.944491 -92.326012
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