Coverart for item
The Resource Assay development : fundamentals and practices, Ge Wu

Assay development : fundamentals and practices, Ge Wu

Label
Assay development : fundamentals and practices
Title
Assay development
Title remainder
fundamentals and practices
Statement of responsibility
Ge Wu
Creator
Subject
Language
eng
Summary
"Essential principles and practice of assay development. The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss: The basics of assay development, including foundational concepts and applications ; Commonly used instrumental methods for both biochemical assays and cell-based assays ; Assay strategies for protein binding and enzymatic activity ; Cell-based assays ; High-throughput screening. An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process."--Publisher's website
Cataloging source
DLC
http://library.link/vocab/creatorDate
1962-
http://library.link/vocab/creatorName
Wu, Ge
Dewey number
612/.015
Illustrations
  • illustrations
  • plates
Index
index present
LC call number
QH90.57.B5
LC item number
W84 2010
Literary form
non fiction
Nature of contents
bibliography
http://library.link/vocab/subjectName
Biological assay
Label
Assay development : fundamentals and practices, Ge Wu
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Introduction to assay development -- Measurement and Instrumentation -- Fundamental principles of assay with isolated proteins -- Separation-based techniques in bioassays -- General protein binding assay formats -- Functional assays with isolated proteases -- Functional assays with isolated kinases -- Fundamental principles of cell-based assays -- Assays with ion channels -- Assays with G protein-coupled receptors -- Assays based on the integrated system properties of cells -- High content cell-based assay with optical imaging techniques -- High throughput screening -- A case study: The development of a microfluidic-based kinase assay platform
Control code
430522233
Dimensions
25 cm
Extent
xx, 425 pages, 4 unnumbered pages of plates
Isbn
9780470191156
Isbn Type
(cloth)
Lccn
2009031386
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)
System control number
(OCoLC)430522233
Label
Assay development : fundamentals and practices, Ge Wu
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Introduction to assay development -- Measurement and Instrumentation -- Fundamental principles of assay with isolated proteins -- Separation-based techniques in bioassays -- General protein binding assay formats -- Functional assays with isolated proteases -- Functional assays with isolated kinases -- Fundamental principles of cell-based assays -- Assays with ion channels -- Assays with G protein-coupled receptors -- Assays based on the integrated system properties of cells -- High content cell-based assay with optical imaging techniques -- High throughput screening -- A case study: The development of a microfluidic-based kinase assay platform
Control code
430522233
Dimensions
25 cm
Extent
xx, 425 pages, 4 unnumbered pages of plates
Isbn
9780470191156
Isbn Type
(cloth)
Lccn
2009031386
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)
System control number
(OCoLC)430522233

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