Coverart for item
The Resource Auditory and vestibular research : methods and protocols, edited by Bernd Sokolowski

Auditory and vestibular research : methods and protocols, edited by Bernd Sokolowski

Label
Auditory and vestibular research : methods and protocols
Title
Auditory and vestibular research
Title remainder
methods and protocols
Statement of responsibility
edited by Bernd Sokolowski
Contributor
Subject
Language
eng
Summary
While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders
Member of
Cataloging source
CUS
Dewey number
612.858
Illustrations
illustrations
Index
index present
LC call number
QP471
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
WV 270 A912 2009
http://library.link/vocab/relatedWorkOrContributorName
Sokolowski, Bernd
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
493
http://library.link/vocab/subjectName
  • Vestibular apparatus
  • Hearing disorders
  • Equilibrium (Physiology)
  • Auditory Pathways
  • Ear Diseases
  • Vestibular Diseases
  • Vestibule, Labyrinth
  • Equilibrium (Physiology)
  • Hearing disorders
  • Vestibular apparatus
Label
Auditory and vestibular research : methods and protocols, edited by Bernd Sokolowski
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio [and others] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande [and others] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico [and others] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan [and others] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith [and others] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada
Control code
276997936
Dimensions
unknown
Extent
1 online resource (xii, 421 pages)
Form of item
online
Isbn
9781597455237
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)276997936
Label
Auditory and vestibular research : methods and protocols, edited by Bernd Sokolowski
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio [and others] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande [and others] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico [and others] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan [and others] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith [and others] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada
Control code
276997936
Dimensions
unknown
Extent
1 online resource (xii, 421 pages)
Form of item
online
Isbn
9781597455237
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)276997936

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...