Coverart for item
The Resource Avidin-biotin interactions : methods and applications, edited by Robert J. McMahon

Avidin-biotin interactions : methods and applications, edited by Robert J. McMahon

Label
Avidin-biotin interactions : methods and applications
Title
Avidin-biotin interactions
Title remainder
methods and applications
Statement of responsibility
edited by Robert J. McMahon
Contributor
Subject
Language
eng
Summary
For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology
Member of
Is part of
Cataloging source
VVL
Dewey number
572.58
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP552.A94
LC item number
A95 2008
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 55.4
NLM item number
  • ME9616J v.418 2008
  • A957 2008
http://library.link/vocab/relatedWorkOrContributorDate
1958-
http://library.link/vocab/relatedWorkOrContributorName
McMahon, Robert Joseph
Series statement
Methods in molecular biology
Series volume
v. 418
http://library.link/vocab/subjectName
  • Affinity chromatography
  • Affinity electrophoresis
  • Avidin
  • Biotin
  • Avidin
  • Biotin
  • Streptavidin
  • Protein Binding
  • Reagent Kits, Diagnostic
  • Affinity Labels
  • Affinity chromatography
  • Affinity electrophoresis
  • Avidin
  • Biotin
Label
Avidin-biotin interactions : methods and applications, edited by Robert J. McMahon
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation of avidin conjugates / Rosaria P. Haugland and Mahesh K. Bhalgat -- Coupling of antibodies with biotin / Rosaria P. Haugland and Wendy W. You -- Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin / Sara R. Zwart and Brandon J. Lewis -- Use of the avidin (Imino) biotin system as a general approach to affinity precipitation / Ruth Freitag and Frank Hilbrig -- Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis / Bao-Shiang Lee [and others] -- Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein / Nicolas Humbert and Thomas R. Ward -- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives / Andreas Ebner [and others] -- quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins / Christoph Rösli [and others] -- High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli / Nicolas Humbert [and others] -- Endogenous biotin in rat brain : implications for false-positive results with avidin-biotin and streptavidin-biotin techniques / Bruce E. McKay, Michael L. Molineux, and Ray W. Turner -- Pseudo-immunolabelling with the avidin-biotin-peroxidase complex due to the presence of endogenous biotin in the retina / Willem Kamphuis and Jan Klooster -- Use of synthetic peptides for identifying biotinylation sites in human histones / Gabriela Camporeale [and others] -- Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay / Kirsten Liebert and Albert Jeltsch -- Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin / Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux -- Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagnostic procedure / Karel Smetana, Jr. and Sabine André -- A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin / Krista McCutcheon -- Biotin-protein bond : instability and structural modification to provide stability for in vivo applications / Donald M. Mock and Anna Bogusiewicz
Control code
262555461
Dimensions
unknown
Extent
1 online resource (xiii, 232 pages)
Form of item
online
Isbn
9781597455794
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-59745-579-4
Other physical details
illustrations.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)262555461
Label
Avidin-biotin interactions : methods and applications, edited by Robert J. McMahon
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation of avidin conjugates / Rosaria P. Haugland and Mahesh K. Bhalgat -- Coupling of antibodies with biotin / Rosaria P. Haugland and Wendy W. You -- Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin / Sara R. Zwart and Brandon J. Lewis -- Use of the avidin (Imino) biotin system as a general approach to affinity precipitation / Ruth Freitag and Frank Hilbrig -- Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis / Bao-Shiang Lee [and others] -- Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein / Nicolas Humbert and Thomas R. Ward -- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives / Andreas Ebner [and others] -- quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins / Christoph Rösli [and others] -- High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli / Nicolas Humbert [and others] -- Endogenous biotin in rat brain : implications for false-positive results with avidin-biotin and streptavidin-biotin techniques / Bruce E. McKay, Michael L. Molineux, and Ray W. Turner -- Pseudo-immunolabelling with the avidin-biotin-peroxidase complex due to the presence of endogenous biotin in the retina / Willem Kamphuis and Jan Klooster -- Use of synthetic peptides for identifying biotinylation sites in human histones / Gabriela Camporeale [and others] -- Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay / Kirsten Liebert and Albert Jeltsch -- Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin / Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux -- Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagnostic procedure / Karel Smetana, Jr. and Sabine André -- A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin / Krista McCutcheon -- Biotin-protein bond : instability and structural modification to provide stability for in vivo applications / Donald M. Mock and Anna Bogusiewicz
Control code
262555461
Dimensions
unknown
Extent
1 online resource (xiii, 232 pages)
Form of item
online
Isbn
9781597455794
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-59745-579-4
Other physical details
illustrations.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)262555461

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