Coverart for item
The Resource Bacterial artificial chromosomes, Volume 1, Library construction, physical mapping, and sequencing, edited by Shaying Zhao and Marvin Stodolsky

Bacterial artificial chromosomes, Volume 1, Library construction, physical mapping, and sequencing, edited by Shaying Zhao and Marvin Stodolsky

Label
Bacterial artificial chromosomes, Volume 1, Library construction, physical mapping, and sequencing
Title
Bacterial artificial chromosomes
Title number
Volume 1
Title part
Library construction, physical mapping, and sequencing
Statement of responsibility
edited by Shaying Zhao and Marvin Stodolsky
Title variation
Library construction, physical mapping, and sequencing
Contributor
Subject
Genre
Language
eng
Summary
The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes
Member of
Cataloging source
N$T
Dewey number
660.6/5
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QH600.15
LC item number
.B338eb vol. 1
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 255-256
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QW 51
NLM item number
  • ME9616J v.255-256 2004
  • B129 2004
http://library.link/vocab/relatedWorkOrContributorName
  • Zhao, Shaying
  • Stodolsky, Marvin
Series statement
Methods in molecular biology
Series volume
v. 255
http://library.link/vocab/subjectName
  • Bacterial artificial chromosomes
  • Chromosomes, Artificial, Bacterial
  • Chromosome Mapping
  • Cloning, Molecular
  • Genomic Library
  • SCIENCE
  • Bacterial artificial chromosomes
Label
Bacterial artificial chromosomes, Volume 1, Library construction, physical mapping, and sequencing, edited by Shaying Zhao and Marvin Stodolsky
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
BAC library construction / K. Osoegawa and P.J. de Jong -- Construction of small genome BAC library for functional and genomic applications / D. Smajs, S.J. Norris and G.M. Weinstock -- Preparation of BAC libraries from bacterial genomes by in vitro packaging / S. Pal, S. Mak and G.M. Weinstock -- Exploring transformation-associated recombination cloning for selective isolation of genomic regions / N. Kouprina [and others] -- Purification of BAC DNA / T.S. Poulsen -- Hybridization-based selection of BAC clones / C.S. Lim and U.J. Kim -- Applications of interspersed repeat sequence polymerase chain reaction / H. Zimdahl [and others] -- BAC mapping using fluorescence in situ hybridization / X.N. Chen and J.R. Korenberg -- High-throughput BAC fingerprinting / J. Schein [and others] -- BAC end sequencing / T.S. Poulsen and H.E. Johnsen -- Radiation hybrid mapping with BAC ends / M. Olivier, S. Brady and D.R. Cox -- Shotgun library construction for DNA sequencing / B.A. Roe -- Rolling circle amplification for sequencing templates / P.F. Predki [and others] -- Transposon-mediated sequencing / R. Reeg and A. Madan -- Pyrosequencing: A tool for DNA sequencing analysis / E. Elahi and M. Ronaghi -- Use of fimers to eliminate polymerase chain reaction and primer-dimer artifacts and to increase yield in bac-sequencing reactions / A. Malykh [and others] -- Retrofitting bacs and pacs with loxP transposons to generate nested deletions / P.K. Chatterjee -- Preparing nested-deletion template DNA for field inversion gel electrophoresis analyses and position-specific end sequencing with transposon primers / P.K. Chatterjee and J.C. Baker, Jr. -- BAC finishing strategies / C. Bird and D. Grafham -- Using the TIGR assembler in shotgun sequencing projects / M. Pop and D. Kosack -- Finishing "working draft" BAC projects by directed sequencing with thermofidelase and fimers / A. Malykh [and others] -- Optimized multiplex polymerase chain reaction: An effective method for rapid gap closure / D. Radune and H. Tettelin -- Assembly of DNA sequencing data / J. Schmutz, J. Grimwood and R.M. Myers -- Sequence finishing / J. Schmutz, J. Grimwood and R.M. Myers -- Quality assessment of finished BAC sequences / J. Schmutz, J. Grimwood and R.M. Myers
Control code
56722553
Dimensions
unknown
Extent
1 online resource (1 volume)
File format
unknown
Form of item
online
Isbn
9781592597529
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)56722553
Label
Bacterial artificial chromosomes, Volume 1, Library construction, physical mapping, and sequencing, edited by Shaying Zhao and Marvin Stodolsky
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
BAC library construction / K. Osoegawa and P.J. de Jong -- Construction of small genome BAC library for functional and genomic applications / D. Smajs, S.J. Norris and G.M. Weinstock -- Preparation of BAC libraries from bacterial genomes by in vitro packaging / S. Pal, S. Mak and G.M. Weinstock -- Exploring transformation-associated recombination cloning for selective isolation of genomic regions / N. Kouprina [and others] -- Purification of BAC DNA / T.S. Poulsen -- Hybridization-based selection of BAC clones / C.S. Lim and U.J. Kim -- Applications of interspersed repeat sequence polymerase chain reaction / H. Zimdahl [and others] -- BAC mapping using fluorescence in situ hybridization / X.N. Chen and J.R. Korenberg -- High-throughput BAC fingerprinting / J. Schein [and others] -- BAC end sequencing / T.S. Poulsen and H.E. Johnsen -- Radiation hybrid mapping with BAC ends / M. Olivier, S. Brady and D.R. Cox -- Shotgun library construction for DNA sequencing / B.A. Roe -- Rolling circle amplification for sequencing templates / P.F. Predki [and others] -- Transposon-mediated sequencing / R. Reeg and A. Madan -- Pyrosequencing: A tool for DNA sequencing analysis / E. Elahi and M. Ronaghi -- Use of fimers to eliminate polymerase chain reaction and primer-dimer artifacts and to increase yield in bac-sequencing reactions / A. Malykh [and others] -- Retrofitting bacs and pacs with loxP transposons to generate nested deletions / P.K. Chatterjee -- Preparing nested-deletion template DNA for field inversion gel electrophoresis analyses and position-specific end sequencing with transposon primers / P.K. Chatterjee and J.C. Baker, Jr. -- BAC finishing strategies / C. Bird and D. Grafham -- Using the TIGR assembler in shotgun sequencing projects / M. Pop and D. Kosack -- Finishing "working draft" BAC projects by directed sequencing with thermofidelase and fimers / A. Malykh [and others] -- Optimized multiplex polymerase chain reaction: An effective method for rapid gap closure / D. Radune and H. Tettelin -- Assembly of DNA sequencing data / J. Schmutz, J. Grimwood and R.M. Myers -- Sequence finishing / J. Schmutz, J. Grimwood and R.M. Myers -- Quality assessment of finished BAC sequences / J. Schmutz, J. Grimwood and R.M. Myers
Control code
56722553
Dimensions
unknown
Extent
1 online resource (1 volume)
File format
unknown
Form of item
online
Isbn
9781592597529
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)56722553

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