Coverart for item
The Resource Basic DNA and RNA protocols, edited by Adrian J. Harwood

Basic DNA and RNA protocols, edited by Adrian J. Harwood

Label
Basic DNA and RNA protocols
Title
Basic DNA and RNA protocols
Statement of responsibility
edited by Adrian J. Harwood
Contributor
Subject
Genre
Language
eng
Summary
An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. The collection includes not only many updated and improved classic techniques, but also a powerful group of advanced methods that point to future progress, among them nonisotopic DNA labeling, silver staining, and automatic sequencing. This excellent bench companion will help those who need to learn for the first time how to conduct research on the molecular biology of nucleic acids or those who need to broaden their competence and laboratory skills. Even highly skilled researchers will find many time-saving techniques
Member of
Action
digitized
Cataloging source
YUS
Dewey number
574.87/328/0724
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP620
LC item number
.B38 1996
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.58
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QZ 50
NLM item number
  • ME9616J v.58 1996
  • B311 1996
http://library.link/vocab/relatedWorkOrContributorName
Harwood, Adrian J
Series statement
Methods in molecular biology,
Series volume
v. 58
http://library.link/vocab/subjectName
  • Nucleic acids
  • Nucleic Acids
  • DNA
  • RNA
  • Genetic Engineering
  • Genetic Techniques
  • Nucleic acids
  • DNA
  • RNA
  • Wetenschappelijke technieken
  • Human Anatomy & Physiology
  • Animal Biochemistry
  • Health & Biological Sciences
Label
Basic DNA and RNA protocols, edited by Adrian J. Harwood
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed 32P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe
Control code
54642635
Dimensions
unknown
Extent
1 online resource (xiii, 514 pages)
File format
unknown
Form of item
online
Isbn
9781592592517
Lccn
95050258
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/089603402X
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)54642635
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Basic DNA and RNA protocols, edited by Adrian J. Harwood
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed 32P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe
Control code
54642635
Dimensions
unknown
Extent
1 online resource (xiii, 514 pages)
File format
unknown
Form of item
online
Isbn
9781592592517
Lccn
95050258
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/089603402X
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)54642635
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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