The Resource Basic DNA and RNA protocols, edited by Adrian J. Harwood
Basic DNA and RNA protocols, edited by Adrian J. Harwood
Resource Information
The item Basic DNA and RNA protocols, edited by Adrian J. Harwood represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Basic DNA and RNA protocols, edited by Adrian J. Harwood represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Language
- eng
- Extent
- xiii, 514 pages
- Contents
-
- The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau
- Restriction endonuclease digestion of DNA / Duncan R. Smith
- Agarose gel electrophoresis / Duncan R. Smith
- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy
- Random primed 32P-labeling of DNA / Duncan R. Smith
- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell
- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery
- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris
- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham
- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham
- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey
- The preparation of riboprobes / Dominique Belin
- Native polyacrylamide gel electrophoresis / Adrian J. Harwood
- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril
- End-labeling of DNA fragments / Adrian J. Harwood
- Northern blot analysis / Robb Krumlauf
- RNA slot blotting / David Murphy
- The RNase protection assay / Dominique Belin
- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient
- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani
- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant
- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway
- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard
- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk
- Construction of cDNA libraries / Michael M. Burrell
- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood
- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff
- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly
- Transformation of E. coli / Fiona M. Tomley
- Transformation of bacteria by electroporation / Lucy Drury
- Preparation of plasmid DNA using alkaline lysis / Etienne Joly
- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood
- Plasmid preparations with diatomaceous earth / Laura Machesky
- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White
- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman
- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston
- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar
- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer
- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower
- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem
- Cloning into M13 bacteriophage vectors / Qingzhong Yu
- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff
- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley
- Preparation of ssDNA from phagemid vectors / Michael K. Trower
- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln
- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin
- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury
- Electrophoresis of sequence reaction samples / Alan T. Bankier
- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear
- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko
- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson
- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky
- DNA sequencing by the chemical method / Eran Pichersky
- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray
- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower
- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd
- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd
- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe
- Isbn
- 9780896034020
- Label
- Basic DNA and RNA protocols
- Title
- Basic DNA and RNA protocols
- Statement of responsibility
- edited by Adrian J. Harwood
- Subject
-
- ADN -- Analyse
- ARN -- Analyse
- Acides nucléiques -- Analyse
- Acides nucléiques -- Technique
- Biologie moléculaire -- Technique
- Clonage moléculaire -- Technique
- DNA
- Genetic Techniques
- Handbooks and manuals
- Mutagenèse dirigée -- Technique
- Nucleic acids -- Laboratory manuals
- RNA
- Réaction en chaîne de la polymérase -- Technique
- Séquence nucléotidique -- Technique
- Wetenschappelijke technieken
- Language
- eng
- Cataloging source
- DLC
- Dewey number
- 574.87/328/0724
- Illustrations
- illustrations
- Index
- index present
- LC call number
- QP620
- LC item number
- .B38 1996
- Literary form
- non fiction
- NAL call number
- QH506.M45
- NAL item number
- v.58
- Nature of contents
- bibliography
- NLM call number
-
- W1
- QZ 50
- NLM item number
-
- ME9616J v.58 1996
- B311 1996
- http://library.link/vocab/relatedWorkOrContributorName
- Harwood, Adrian J
- Series statement
- Methods in molecular biology
- Series volume
- 58
- http://library.link/vocab/subjectName
-
- Nucleic acids
- Genetic Techniques
- DNA
- RNA
- Wetenschappelijke technieken
- Acides nucléiques
- Acides nucléiques
- ADN
- ARN
- Clonage moléculaire
- Réaction en chaîne de la polymérase
- Séquence nucléotidique
- Mutagenèse dirigée
- Biologie moléculaire
- Label
- Basic DNA and RNA protocols, edited by Adrian J. Harwood
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- volume
- Carrier category code
-
- nc
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed 32P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe
- Control code
- 33818385
- Dimensions
- 23 cm
- Extent
- xiii, 514 pages
- Isbn
- 9780896034020
- Isbn Type
- (hc : alk. paper)
- Lccn
- 95050258
- Media category
- unmediated
- Media MARC source
- rdamedia
- Media type code
-
- n
- Other physical details
- illustrations
- System control number
- (OCoLC)33818385
- Label
- Basic DNA and RNA protocols, edited by Adrian J. Harwood
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- volume
- Carrier category code
-
- nc
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction endonuclease digestion of DNA / Duncan R. Smith -- Agarose gel electrophoresis / Duncan R. Smith -- Capillary blotting of agarose gels / Duncan R. Smith, David Murphy -- Random primed 32P-labeling of DNA / Duncan R. Smith -- Hybridization and competition hybridization of Southern blots / Rosemary E. Kelsell -- Utilization of DNA probes with digoxigenin-modified nucleotides in Southern hybridizations / Tim Helentjaris, Tom McCreery -- The preparation of fluorescein-labeled nucleic acid probes and their detection using alkaline phosphatase-catalyzed dioxetane chemiluminescence / Martin Cunningham, Martin Harris -- Monitoring incorporation of fluorescein into nucleic acid probes using a rapid labeling assay / Martin Cunningham -- The preparation of horseradish peroxidase-labeled nucleic acid probes and their detection using enhanced chemiluminescence / Bronwen Harvey, Ian Durrant, Martin Cunningham -- 3'-end labeling of oligonucleotides with fluorescein-11-dUTP and enhanced chemiluminescent detection / Martin Cunningham, Ian Durrant, Bronwen Harvey -- The preparation of riboprobes / Dominique Belin -- Native polyacrylamide gel electrophoresis / Adrian J. Harwood -- Use of silver staining to detect nucleic acids / Lloyd G. Mitchell, Angelika Bodenteich, Carl R. Merril -- End-labeling of DNA fragments / Adrian J. Harwood -- Northern blot analysis / Robb Krumlauf -- RNA slot blotting / David Murphy -- The RNase protection assay / Dominique Belin -- Primer extension analysis of mRNA / Mark W. Leonard, Roger K. Patient -- S1 mapping using single-stranded DNA probes / Stéphane Viville, Roberto Mantovani -- Nonradioactive in situ hybridization for cells and tissues / Ian Durrant -- In vitro packaging of DNA / Jeremy W. Dale, Peter J. Greenaway -- Construction of mammalian genomic libraries using [lambda] replacement vectors / Alan N. Bateson, Jeffrey W. Pollard -- The production of double-stranded complementary DNA for use in making libraries / Steve Mayall, Jane Kirk -- Construction of cDNA libraries / Michael M. Burrell -- Screening [lambda] libraries / Janet C. Harwood, Adrian J. Harwood -- Subcloning strategies and protocols / Danielle Gioioso Taghian, Jac A. Nickoloff -- Purification of DNA fragments from agarose gels using glass beads / Etienne Joly -- Transformation of E. coli / Fiona M. Tomley -- Transformation of bacteria by electroporation / Lucy Drury -- Preparation of plasmid DNA using alkaline lysis / Etienne Joly -- The rapid boiling method for small-scale preparation of plasmid DNA / Adrian J. Harwood -- Plasmid preparations with diatomaceous earth / Laura Machesky -- Polymerase chain reaction / Beverly C. Delidow, John P. Lynch, John J. Peluso, Bruce A. White -- Inverse polymerase chain reaction / Daniel L. Hartl, Howard Ochman -- Polymerase chain reaction with degenerate oligonucleotide primers to clone gene family members / Gregory M. Preston -- Cloning PCR products using T-vectors / Michael K. Trower, Greg S. Elgar -- Direct radioactive labeling of polymerase chain reaction products / Tim McDaniel, Stephen J. Meltzer -- A rapid PCR-based colony screening protocol for cloned inserts / Michael K. Trower -- Use of polymerase chain reaction to screen phage libraries / Lei Yu, Laura J. Bloem -- Cloning into M13 bacteriophage vectors / Qingzhong Yu -- Ordered deletions using exonuclease III / Denise Clark, Steven Henikoff -- M13 phage growth and single-stranded DNA preparation / Fiona M. Tomley -- Preparation of ssDNA from phagemid vectors / Michael K. Trower -- A rapid plasmid purification method for dideoxy sequencing / Annette M. Grifln, Hugh G. Grifln -- DNA sequencing using sequenase version 2.0 T7 DNA polymerase / Carl W. Fuller, Bernard F. McArdle, Annette M. Griffin, Hugh G. Griffin -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Direct sequencing of PCR products / Janet C. Harwood, Geraldine A. Phear -- Thermal cycle dideoxy DNA sequencing / Barton E. Slatko -- Using the automated DNA sequencer / Zijin Du, Richard K. Wilson -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites / Jac A. Nickoloff, Win-Ping Deng, Elizabeth M. Miller, F. Andrew Ray -- A protocol for site-directed mutagenesis employing a uracil-containing phagemid template / Michael K. Trower -- In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system / Louise Olliver, Charles D. Boyd -- In vitro translation of messenger RNA in a wheat germ extract cell-free system / Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd -- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin / Joanne Crowe, Brigitte Steude Masone, Joachim Ribbe
- Control code
- 33818385
- Dimensions
- 23 cm
- Extent
- xiii, 514 pages
- Isbn
- 9780896034020
- Isbn Type
- (hc : alk. paper)
- Lccn
- 95050258
- Media category
- unmediated
- Media MARC source
- rdamedia
- Media type code
-
- n
- Other physical details
- illustrations
- System control number
- (OCoLC)33818385
Subject
- ADN -- Analyse
- ARN -- Analyse
- Acides nucléiques -- Analyse
- Acides nucléiques -- Technique
- Biologie moléculaire -- Technique
- Clonage moléculaire -- Technique
- DNA
- Genetic Techniques
- Handbooks and manuals
- Mutagenèse dirigée -- Technique
- Nucleic acids -- Laboratory manuals
- RNA
- Réaction en chaîne de la polymérase -- Technique
- Séquence nucléotidique -- Technique
- Wetenschappelijke technieken
Genre
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Basic-DNA-and-RNA-protocols-edited-by-Adrian-J./K0guYNOZWBs/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Basic-DNA-and-RNA-protocols-edited-by-Adrian-J./K0guYNOZWBs/">Basic DNA and RNA protocols, edited by Adrian J. Harwood</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
