Coverart for item
The Resource Basic protein and peptide protocols, edited by John M. Walker

Basic protein and peptide protocols, edited by John M. Walker

Label
Basic protein and peptide protocols
Title
Basic protein and peptide protocols
Statement of responsibility
edited by John M. Walker
Contributor
Subject
Language
eng
Member of
Cataloging source
DLC
Dewey number
574.19/245
Illustrations
illustrations
Index
index present
LC call number
QP551
LC item number
.B268 1994
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
no.32
Nature of contents
bibliography
NLM call number
  • W1
  • QU 55
NLM item number
  • ME9616J v.32 1994
  • B311 1994
http://library.link/vocab/relatedWorkOrContributorDate
1948-
http://library.link/vocab/relatedWorkOrContributorName
Walker, John M.
Series statement
Methods in molecular biology
Series volume
32
http://library.link/vocab/subjectName
  • Proteins
  • Peptides
  • Peptides
  • Proteins
  • Eiwitten
  • Peptiden
  • Analyse (algemeen)
  • Wetenschappelijke technieken
  • Proteínas
  • Péptidos
Label
Basic protein and peptide protocols, edited by John M. Walker
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Lowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension -- Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing -- Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method -- Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis
Control code
29597280
Dimensions
23 cm
Extent
xii, 490 pages
Isbn
9780896032682
Lccn
93048929
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(WaOLN)1681318
Label
Basic protein and peptide protocols, edited by John M. Walker
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Lowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension -- Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing -- Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method -- Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis
Control code
29597280
Dimensions
23 cm
Extent
xii, 490 pages
Isbn
9780896032682
Lccn
93048929
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
System control number
(WaOLN)1681318

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...