Coverart for item
The Resource Bioconjugation protocols : strategies and methods, edited by Christof M. Niemeyer

Bioconjugation protocols : strategies and methods, edited by Christof M. Niemeyer

Label
Bioconjugation protocols : strategies and methods
Title
Bioconjugation protocols
Title remainder
strategies and methods
Statement of responsibility
edited by Christof M. Niemeyer
Contributor
Subject
Genre
Language
eng
Summary
Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanoparticles, and carbon nanotube probes for atomic force microscopy. All the protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods offers both novice and experienced researchers access to the broad array of techniques needed to master the semisynthesis of functional biomolecular reagents for a wide variety of applications-ranging from novel biomedical diagnostics, to powerful new therapeutics, to advanced biomaterials
Member of
Cataloging source
COO
Dewey number
612.1/111
Illustrations
illustrations
Index
index present
LC call number
QP517.B49
LC item number
B565 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QW 25
NLM item number
  • ME9616J v.283 2004
  • B6147 2004
http://library.link/vocab/relatedWorkOrContributorName
Niemeyer, Christof M
Series statement
Methods in molecular biology
Series volume
283
http://library.link/vocab/subjectName
  • Bioconjugates
  • Immunohistochemistry
  • Adjuvants, Immunologic
  • Gene Transfer Techniques
  • Molecular Probes
  • MEDICAL
  • SCIENCE
  • Bioconjugates
Label
Bioconjugation protocols : strategies and methods, edited by Christof M. Niemeyer
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
PART I ANTIBODY AND ENZYME CONJUGATES: Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress / Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov -- Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals / Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano -- Smart Polymer-Streptavidin Conjugates / Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman -- Conjugates of Peptides and Proteins to Polyethylene Glycols / Margherita Morpurgo and Francesco M. Veronese -- Chemical Production of Bispecific Antibodies / Robert F. Graziano and Paul Guptill -- Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties / Carl-Wilhelm Vogel -- Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase Markus Meusel -- PART II NUCLEIC ACID CONJUGATES: Fluorescent Sample Labeling for DNA Microarray Analyses / Verena Beier, Andrea Bauer, Michael Baum, and Jorg D. Hoheisel -- High-Density Labeling of DNA for Single Molecule Sequencing / Susanne Brakmann -- Sequence-Specific DNA Labeling Using Methyltransferases / Goran Pljevaljtic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold -- Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications / Michael Adler -- Covalent Coupling of DNA Oligonucleotides and Streptavidin / Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer -- Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates / David R. Corey -- Synthesis of Peptide Nucleic Acid-Peptide Conjugates / Kunihiro Kaihatsu and David R. Corey -- PART III GLYCOSYL AND LIPID CONJUGATES: Protein Lipidation / lirgen Kuhlmann -- Synthesis of Lipidated Peptides / Ines Heinemann, Martin Volkert, and Herbert Waldmann -- In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins / Thomas Durek, Roger S. Goody, and Kirill Alexandrov -- Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling / Melanie Wagner and iirgen Kuhlmann -- Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 / Thomas J. Tolbert and Chi-Huey Wong -- Subtilisin-Catalyzed Glycopeptide Condensation / Thomas J. Tolbert and Chi-Huey Wong -- PART IV BIOFUNCTIONALIZATION OF SURFACES: Peptide Nucleic Acid Microarrays / Anette Jacob, Ole Brandt, Achim Stephan, and forg D. Hoheisel ... 283 22 Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles / Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer -- Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging / Adam T. Woolley
Control code
56471027
Dimensions
unknown
Extent
1 online resource (xi, 330 pages)
Form of item
online
Isbn
9781588290984
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)56471027
Label
Bioconjugation protocols : strategies and methods, edited by Christof M. Niemeyer
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
PART I ANTIBODY AND ENZYME CONJUGATES: Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress / Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov -- Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals / Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano -- Smart Polymer-Streptavidin Conjugates / Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman -- Conjugates of Peptides and Proteins to Polyethylene Glycols / Margherita Morpurgo and Francesco M. Veronese -- Chemical Production of Bispecific Antibodies / Robert F. Graziano and Paul Guptill -- Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties / Carl-Wilhelm Vogel -- Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase Markus Meusel -- PART II NUCLEIC ACID CONJUGATES: Fluorescent Sample Labeling for DNA Microarray Analyses / Verena Beier, Andrea Bauer, Michael Baum, and Jorg D. Hoheisel -- High-Density Labeling of DNA for Single Molecule Sequencing / Susanne Brakmann -- Sequence-Specific DNA Labeling Using Methyltransferases / Goran Pljevaljtic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold -- Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications / Michael Adler -- Covalent Coupling of DNA Oligonucleotides and Streptavidin / Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer -- Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates / David R. Corey -- Synthesis of Peptide Nucleic Acid-Peptide Conjugates / Kunihiro Kaihatsu and David R. Corey -- PART III GLYCOSYL AND LIPID CONJUGATES: Protein Lipidation / lirgen Kuhlmann -- Synthesis of Lipidated Peptides / Ines Heinemann, Martin Volkert, and Herbert Waldmann -- In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins / Thomas Durek, Roger S. Goody, and Kirill Alexandrov -- Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling / Melanie Wagner and iirgen Kuhlmann -- Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 / Thomas J. Tolbert and Chi-Huey Wong -- Subtilisin-Catalyzed Glycopeptide Condensation / Thomas J. Tolbert and Chi-Huey Wong -- PART IV BIOFUNCTIONALIZATION OF SURFACES: Peptide Nucleic Acid Microarrays / Anette Jacob, Ole Brandt, Achim Stephan, and forg D. Hoheisel ... 283 22 Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles / Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer -- Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging / Adam T. Woolley
Control code
56471027
Dimensions
unknown
Extent
1 online resource (xi, 330 pages)
Form of item
online
Isbn
9781588290984
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)56471027

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