Coverart for item
The Resource Bone research protocols, edited by Miep H. Helfrich, Stuart H. Ralston

Bone research protocols, edited by Miep H. Helfrich, Stuart H. Ralston

Label
Bone research protocols
Title
Bone research protocols
Statement of responsibility
edited by Miep H. Helfrich, Stuart H. Ralston
Contributor
Subject
Genre
Language
eng
Summary
The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism
Member of
Cataloging source
COO
Dewey number
612.7/5/072
Illustrations
illustrations
Index
index present
LC call number
QP88.2
LC item number
.B5898 2003
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • WE 200
NLM item number
  • ME9616JM v.80 2003
  • B7129 2003
http://library.link/vocab/relatedWorkOrContributorName
  • Helfrich, Miep H
  • Ralston, Stuart
Series statement
Methods in molecular medicine
Series volume
80
http://library.link/vocab/subjectName
  • Bones
  • Bone cells
  • Bone and Bones
  • Cell Culture Techniques
  • Histological Techniques
  • Osteoblasts
  • Osteoclasts
  • MEDICAL
  • SCIENCE
  • Bone cells
  • Bot (anatomie)
  • Wetenschappelijke technieken
  • Gidsen (vorm)
Label
Bone research protocols, edited by Miep H. Helfrich, Stuart H. Ralston
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Human osteoblast culture / J.A. Gallagher -- Osteoblast isolation from murine calvariae and long bones / A. Bakker and J. Klein-Nulend -- Mineralizing fibroblast-colony-forming assays / A. Scutt [and others] -- Osteocyte isolation and culture / P.J. Nijweide [and others] -- Isolated osteoclast cultures / A. Hoebertz and T.R. Arnett -- Primary isolation and culture of chicken osteoclasts / P. Collin-Osdoby, F. Anderson and P. Osdoby -- Isolation and purification of rabbit osteoclasts / F.P. Coxon [and others] -- Generating human osteoclasts from peripheral blood / A. Sabokbar and N.S. Athanasou -- Generating human osteoclasts in vitro from bone marrow and peripheral blood / A.M. Flanagan and H.M. Massey -- Generating murine osteoclasts from bone marrow / N. Takahashi [and others] -- Osteoclast formation in the mouse coculture assay / R.J. van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / P. Collin-Osdoby [and others] -- Analysis of osteoclast function in mouse calvarial cultures / M.J. Marshall and M.N. Rowlands -- Assessing bone formation using mouse calvarial organ cultures / I.R. Garrett -- In situ hybridization and in situ reverse transcription polymerase chain reaction in human bone sections / A.P. Mee and J.A. Hoyland -- Techniques for the study of apoptosis in bone / B.S. Noble and H.Y. Stevens -- Protein localization in wax-embedded and frozen sections of bone using immunohistochemistry / S. Bord -- Detection of noncollagenous bone proteins in methylmethacrylate-embedded human bone sections / J.P. van Leeuwen and P. Derkx -- Fluorescence imaging of bone-resorbing osteoclasts by confocal microscopy / S.A. Nesbitt and M.A. Horton -- Bone histomorphometry / S. Vedi and J. Compston -- Transmission electron microscopy of bone / V. Everts, A. Niehof and W. Beertsen -- Scanning electron microscopy of bone / D. Marshall, M.H. Helfrich and R.M. Aspden -- Bone measurements by peripheral quantitative computed tomography in rodents / J.A. Gasser -- Studies of local bone remodeling. The calvarial injection assay / R.J. van 't Hof, C.E. Clarkin and K.J. Armour -- Inflammation-induced osteoporosis. The IMO model / K.J. Armour and K.E. Armour -- Ovariectomy and estrogen replacement in rodents / J.W. Chow -- Mechanical testing of bone ex vivo / R.M. Aspden -- Bone cell responses to fluid flow / H.Y. Stevens and J.A. Frangos -- Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models / A.A. Pitsillides [and others] -- Extraction of nucleic acids from bone / T.L. Stewart and V. Mann -- Analysis of gene expression in bone by quantitative RT-PCR / D. Ireland
Control code
170734795
Dimensions
unknown
Extent
1 online resource (xiv, 448 pages)
Form of item
online
Isbn
9781592593668
Lccn
2003044967
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)170734795
Label
Bone research protocols, edited by Miep H. Helfrich, Stuart H. Ralston
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Human osteoblast culture / J.A. Gallagher -- Osteoblast isolation from murine calvariae and long bones / A. Bakker and J. Klein-Nulend -- Mineralizing fibroblast-colony-forming assays / A. Scutt [and others] -- Osteocyte isolation and culture / P.J. Nijweide [and others] -- Isolated osteoclast cultures / A. Hoebertz and T.R. Arnett -- Primary isolation and culture of chicken osteoclasts / P. Collin-Osdoby, F. Anderson and P. Osdoby -- Isolation and purification of rabbit osteoclasts / F.P. Coxon [and others] -- Generating human osteoclasts from peripheral blood / A. Sabokbar and N.S. Athanasou -- Generating human osteoclasts in vitro from bone marrow and peripheral blood / A.M. Flanagan and H.M. Massey -- Generating murine osteoclasts from bone marrow / N. Takahashi [and others] -- Osteoclast formation in the mouse coculture assay / R.J. van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / P. Collin-Osdoby [and others] -- Analysis of osteoclast function in mouse calvarial cultures / M.J. Marshall and M.N. Rowlands -- Assessing bone formation using mouse calvarial organ cultures / I.R. Garrett -- In situ hybridization and in situ reverse transcription polymerase chain reaction in human bone sections / A.P. Mee and J.A. Hoyland -- Techniques for the study of apoptosis in bone / B.S. Noble and H.Y. Stevens -- Protein localization in wax-embedded and frozen sections of bone using immunohistochemistry / S. Bord -- Detection of noncollagenous bone proteins in methylmethacrylate-embedded human bone sections / J.P. van Leeuwen and P. Derkx -- Fluorescence imaging of bone-resorbing osteoclasts by confocal microscopy / S.A. Nesbitt and M.A. Horton -- Bone histomorphometry / S. Vedi and J. Compston -- Transmission electron microscopy of bone / V. Everts, A. Niehof and W. Beertsen -- Scanning electron microscopy of bone / D. Marshall, M.H. Helfrich and R.M. Aspden -- Bone measurements by peripheral quantitative computed tomography in rodents / J.A. Gasser -- Studies of local bone remodeling. The calvarial injection assay / R.J. van 't Hof, C.E. Clarkin and K.J. Armour -- Inflammation-induced osteoporosis. The IMO model / K.J. Armour and K.E. Armour -- Ovariectomy and estrogen replacement in rodents / J.W. Chow -- Mechanical testing of bone ex vivo / R.M. Aspden -- Bone cell responses to fluid flow / H.Y. Stevens and J.A. Frangos -- Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models / A.A. Pitsillides [and others] -- Extraction of nucleic acids from bone / T.L. Stewart and V. Mann -- Analysis of gene expression in bone by quantitative RT-PCR / D. Ireland
Control code
170734795
Dimensions
unknown
Extent
1 online resource (xiv, 448 pages)
Form of item
online
Isbn
9781592593668
Lccn
2003044967
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)170734795

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