Coverart for item
The Resource CDNA libraries : methods and applications, edited by Chaofu Lu, John Browse and James G. Wallis

CDNA libraries : methods and applications, edited by Chaofu Lu, John Browse and James G. Wallis

Label
CDNA libraries : methods and applications
Title
CDNA libraries
Title remainder
methods and applications
Statement of responsibility
edited by Chaofu Lu, John Browse and James G. Wallis
Contributor
Subject
Language
eng
Summary
The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years. In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries. The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology
Member of
Cataloging source
GW5XE
Dewey number
572.8
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP624.5.A57
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.729 2011
http://library.link/vocab/relatedWorkOrContributorName
  • Lu, Chaofu
  • Browse, John
  • Wallis, James G
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
729
http://library.link/vocab/subjectName
  • Antisense DNA
  • DNA, Complementary
  • Antisense DNA
  • Human Anatomy & Physiology
  • Health & Biological Sciences
  • Animal Biochemistry
Label
CDNA libraries : methods and applications, edited by Chaofu Lu, John Browse and James G. Wallis
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation of fungal infection structures from plant tissue by flow cytometry for cell-specific transcriptome analysis / H. Takahara, E. Endl and R. O'Connell -- Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using chum-RNA / H. Nojima and T. Tougan -- Construction of a full-length cDNA library from castor endosperm for high-throughput functional screening / C. Lu, J.G. Wallis and J. Browse -- Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method / S. Kato, M. Oshikawa and K. Ohtoko -- Construction of improved yeast two-hybrid libraries / R.H. Maier, C.J. Maier and K. Onder -- Normalization of full-length-enriched cDNA / E.A. Bogdanova [and others] -- Bioinformatic methods for finding differentially expressed genes in cDNA libraries, applied to the identification of tumour vascular targets / J.M. Herbert [and others] -- Enzymatic production of RNAi libraries from cDNAs and high-throughput selection of effective shRNA expression constructs / K. Sugao and K. Hirose -- Construction of small RNA CDNA libraries for high-throughput sequencing / C. Lu and V. Shedge -- Focusing mutations within random libraries to distinct areas: Protein domain library generation by overlap extension / A. Gratz and J. Jose -- Generation of families of construct variants using golden gate shuffling / C. Engler and S. Marillonnet -- Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function / Y. Kondou [and others] -- Construction of yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- Identification of protein/target molecule interactions using yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- SNP discovery by transcriptome pyrosequencing / W.B. Barbazuk and P.S. Schnable -- RNA-seq analysis of gene expression and alternative splicing by double-random priming strategy / M.T. Lovci [and others] -- Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species / D. Bellin, A. Ferrarini and M. Delledonne
Control code
719367736
Dimensions
unknown
Extent
1 online resource (xi, 275 pages)
Form of item
online
Isbn
9781617790652
Lccn
2011922530
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-065-2
Other physical details
illustrations (some color).
http://library.link/vocab/ext/overdrive/overdriveId
978-1-61779-064-5
Specific material designation
remote
System control number
(OCoLC)719367736
Label
CDNA libraries : methods and applications, edited by Chaofu Lu, John Browse and James G. Wallis
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation of fungal infection structures from plant tissue by flow cytometry for cell-specific transcriptome analysis / H. Takahara, E. Endl and R. O'Connell -- Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using chum-RNA / H. Nojima and T. Tougan -- Construction of a full-length cDNA library from castor endosperm for high-throughput functional screening / C. Lu, J.G. Wallis and J. Browse -- Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method / S. Kato, M. Oshikawa and K. Ohtoko -- Construction of improved yeast two-hybrid libraries / R.H. Maier, C.J. Maier and K. Onder -- Normalization of full-length-enriched cDNA / E.A. Bogdanova [and others] -- Bioinformatic methods for finding differentially expressed genes in cDNA libraries, applied to the identification of tumour vascular targets / J.M. Herbert [and others] -- Enzymatic production of RNAi libraries from cDNAs and high-throughput selection of effective shRNA expression constructs / K. Sugao and K. Hirose -- Construction of small RNA CDNA libraries for high-throughput sequencing / C. Lu and V. Shedge -- Focusing mutations within random libraries to distinct areas: Protein domain library generation by overlap extension / A. Gratz and J. Jose -- Generation of families of construct variants using golden gate shuffling / C. Engler and S. Marillonnet -- Application of full-length cDNA resources to gain-of-function technology for characterization of plant gene function / Y. Kondou [and others] -- Construction of yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- Identification of protein/target molecule interactions using yeast surface-displayed cDNA libraries / S. Bidlingmaier and B. Liu -- SNP discovery by transcriptome pyrosequencing / W.B. Barbazuk and P.S. Schnable -- RNA-seq analysis of gene expression and alternative splicing by double-random priming strategy / M.T. Lovci [and others] -- Generation of a large catalog of unique transcripts for whole-genome expression analysis in nonmodel species / D. Bellin, A. Ferrarini and M. Delledonne
Control code
719367736
Dimensions
unknown
Extent
1 online resource (xi, 275 pages)
Form of item
online
Isbn
9781617790652
Lccn
2011922530
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-065-2
Other physical details
illustrations (some color).
http://library.link/vocab/ext/overdrive/overdriveId
978-1-61779-064-5
Specific material designation
remote
System control number
(OCoLC)719367736

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