Coverart for item
The Resource CDNA library protocols, edited by Ian G. Cowell and Caroline A. Austin

CDNA library protocols, edited by Ian G. Cowell and Caroline A. Austin

Label
CDNA library protocols
Title
CDNA library protocols
Statement of responsibility
edited by Ian G. Cowell and Caroline A. Austin
Contributor
Subject
Genre
Language
eng
Summary
This comprehensive collection of detailed protocols covers all areas of cDNA work, from library construction and manipulation to screening and analysis of resulting clones. Great care has been taken to combine up-to-date versions of some of the most widely used protocols with some very useful newer techniques. The protocols describe methods for cloning difficult-to-obtain ends of cDNAs, methods for analyzing cDNA sequence data, and methods for using the wealth of cDNA data emerging from the human genome project. Bearing in mind the importance of the library screening method to the determination of cloning strategy, the book offers a wide range of approaches to screening cDNA libraries
Member of
Is part of
Action
digitized
Cataloging source
YUS
Dewey number
574.87/3282
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QH442.4
LC item number
.C36 1997
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 442.4
NLM item number
  • ME9616J v.69 1997
  • C386 1997
http://library.link/vocab/relatedWorkOrContributorName
  • Cowell, Ian G
  • Austin, Caroline A
Series statement
Methods in molecular biology
Series volume
v. 69
http://library.link/vocab/subjectName
  • Gene libraries
  • DNA
  • Antisense DNA
  • Molecular cloning
  • Genetic Techniques
  • DNA, Complementary
  • Gene Library
  • DNA, Single-Stranded
  • DNA Probes
  • Genetic Structures
  • Investigative Techniques
  • Nucleic Acid Probes
  • DNA
  • Genetic Phenomena
  • Nucleic Acids
  • Phenomena and Processes
  • Molecular Probes
  • Laboratory Chemicals
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Specialty Uses of Chemicals
  • Chemical Actions and Uses
  • Antisense DNA
  • DNA
  • Gene libraries
  • Molecular cloning
  • DNA
  • Kloneren
  • Genetische manipulatie
  • Wetenschappelijke technieken
Label
CDNA library protocols, edited by Ian G. Cowell and Caroline A. Austin
Instantiates
Publication
Note
Source of title; title from title screen
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
cDNA library construction from small amounts of RNA using paramagnetic beads and PCR / Kris N. Lambert, Valerie M. Williamson -- Increasing the average abundance of low-abundance cDNAs by Ordered subdivision of cDNA populations / David R. Sibson, Michael P. Starkey -- Isolation of messenger RNA from plant tissues / Alison Dunn -- cDNA library construction for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Clone excision methods for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Using rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs / Yue Zhang, Michael A. Frohman -- Inverse PCR approach to cloning cDNA ends / Sheng-He Huang -- Cloning gene family members using the polymerase chain reaction with degenerate oligonucleotide primers / Gregory M. Preston -- Subtractive hybridization for the isolation of differentially expressed genes using magnetic beads / Hans-Christian Aasheim, Ton Logtenberg, Frank Larsen -- Preparation of competent cells for high-efficiency plasmid transformation of Escherichia coli / Yasuhiko Nakata, Xiaoren Tang, Kazunari K. Yokoyama -- The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences / Bent Honoé, Peder Madsen -- Screening cDNA libraries by hybridization with double-stranded DNA probes and oligonucleotides / Caroline A. Austin -- Immunological screening of [lambda]-phage cDNA expression libraries / Helen C. Hurst -- Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes / Ian G. Cowell -- Protein interaction cloning by far-western screening of [lambda]-phage cDNA expression libraries / Shinichi Takayama, John C. Reed -- Yeast two-hybrid library screening / Ian G. Cowell -- Signal sequence trap : expression cloning method for secreted proteins and Type 1 membrane proteins / Kei Tashiro, Toru Nakano, Tasuku Honjo -- Isolation of genetic suppressor elements (GSEs) from random fragment cDNA libraries in retroviral vectors / Andrei V. Gudkov, Igor B. Roninson -- Expression and preparation of fusion proteins from recombinant [lambda]gt1.1 phages / Sheng-He Huang, Ambrose Jong -- Computer analysis of cloned sequences / Paul R. Caron -- The TIGR human cDNA database / Ewen F. Kirkness, Anthony R. Kerlavage -- Searching the dbEST database / Patricia Rodriguez-Tomé -- Reference cDNA library facilities available from European sources / Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, Martin J. Bishop
Control code
54645113
Dimensions
unknown
Extent
1 online resource (x, 321 pages)
File format
unknown
Form of item
online
Isbn
9781592595556
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/089603383X
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)54645113
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
CDNA library protocols, edited by Ian G. Cowell and Caroline A. Austin
Publication
Note
Source of title; title from title screen
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
cDNA library construction from small amounts of RNA using paramagnetic beads and PCR / Kris N. Lambert, Valerie M. Williamson -- Increasing the average abundance of low-abundance cDNAs by Ordered subdivision of cDNA populations / David R. Sibson, Michael P. Starkey -- Isolation of messenger RNA from plant tissues / Alison Dunn -- cDNA library construction for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Clone excision methods for the lambda ZAP-based vectors / Marjory A. Snead, Michelle A. Alting-Mees, Jay M. Short -- Using rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs / Yue Zhang, Michael A. Frohman -- Inverse PCR approach to cloning cDNA ends / Sheng-He Huang -- Cloning gene family members using the polymerase chain reaction with degenerate oligonucleotide primers / Gregory M. Preston -- Subtractive hybridization for the isolation of differentially expressed genes using magnetic beads / Hans-Christian Aasheim, Ton Logtenberg, Frank Larsen -- Preparation of competent cells for high-efficiency plasmid transformation of Escherichia coli / Yasuhiko Nakata, Xiaoren Tang, Kazunari K. Yokoyama -- The tetramethylammonium chloride (TMAC) method for screening cDNA libraries with highly degenerate oligonucleotide probes obtained by reverse translation of amino acid sequences / Bent Honoé, Peder Madsen -- Screening cDNA libraries by hybridization with double-stranded DNA probes and oligonucleotides / Caroline A. Austin -- Immunological screening of [lambda]-phage cDNA expression libraries / Helen C. Hurst -- Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes / Ian G. Cowell -- Protein interaction cloning by far-western screening of [lambda]-phage cDNA expression libraries / Shinichi Takayama, John C. Reed -- Yeast two-hybrid library screening / Ian G. Cowell -- Signal sequence trap : expression cloning method for secreted proteins and Type 1 membrane proteins / Kei Tashiro, Toru Nakano, Tasuku Honjo -- Isolation of genetic suppressor elements (GSEs) from random fragment cDNA libraries in retroviral vectors / Andrei V. Gudkov, Igor B. Roninson -- Expression and preparation of fusion proteins from recombinant [lambda]gt1.1 phages / Sheng-He Huang, Ambrose Jong -- Computer analysis of cloned sequences / Paul R. Caron -- The TIGR human cDNA database / Ewen F. Kirkness, Anthony R. Kerlavage -- Searching the dbEST database / Patricia Rodriguez-Tomé -- Reference cDNA library facilities available from European sources / Michael P. Starkey, Yagnesh Umrania, Christopher R. Mundy, Martin J. Bishop
Control code
54645113
Dimensions
unknown
Extent
1 online resource (x, 321 pages)
File format
unknown
Form of item
online
Isbn
9781592595556
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/089603383X
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)54645113
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...