Coverart for item
The Resource Calcium signaling protocols

Calcium signaling protocols

Label
Calcium signaling protocols
Title
Calcium signaling protocols
Title variation
Calcium Signaling Protocols, 3rd ed
Contributor
Contributor
Subject
Genre
Language
eng
Summary
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+
Member of
Cataloging source
LIP
Dewey number
571.6
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP535.C2 C2665 2013
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QV 276
NLM item number
ME9616J v.937 2012
http://library.link/vocab/relatedWorkOrContributorName
  • Lambert, David G
  • Rainbow, Richard D
Series statement
Methods in molecular biology Calcium signaling protocols
http://library.link/vocab/subjectName
  • Calcium
  • Calcium
  • Calcium channels
  • Cellular signal transduction
  • Calcium
  • Calcium Channels
  • Calcium Signaling
  • Fluorometry
  • Signal Transduction
  • Blood Coagulation Factors
  • Ion Transport
  • Luminescent Measurements
  • Metals, Alkaline Earth
  • Second Messenger Systems
  • Ion Channels
  • Biochemical Phenomena
  • Kinetics
  • Cell Physiological Phenomena
  • Publication Formats
  • Biological Science Disciplines
  • Elements
  • Natural Science Disciplines
  • Metals
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Publication Characteristics
  • Biological Factors
  • Biological Transport
  • Chemical Phenomena
  • Photometry
  • Inorganic Chemicals
  • Chemicals and Drugs
  • Disciplines and Occupations
  • Carrier Proteins
  • Chemistry Techniques, Analytical
  • Membrane Proteins
  • Metabolism
  • Phenomena and Processes
  • Proteins
  • Investigative Techniques
  • Amino Acids, Peptides, and Proteins
  • Analytical, Diagnostic and Therapeutic Techniques and Equipment
  • Calcium Signaling
  • Calcium Channels
  • Laboratory Manuals
  • Calcium
  • Pharmacokinetics
  • Physiology
  • Fluorometry
  • Cellular signal transduction
  • Human Anatomy & Physiology
  • Health & Biological Sciences
  • Animal Biochemistry
Label
Calcium signaling protocols
Instantiates
Publication
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Confocal Microscopy: Theory and Applications for Cellular Signaling -- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer -- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®) -- Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader -- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones -- Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells -- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells -- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Calcium Measurements from Whole Heart Using Rhod-2.- Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries -- Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes -- Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors -- Compartmentalizing Genetically-Encoded Calcium Sensors -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Control code
990475994
Dimensions
unknown
Edition
3rd ed. 2013.
Extent
1 online resource (XI, 360 pages 51 illustrations, 17 illustrations in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627030854
Lccn
2012946739
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-086-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)990475994
Label
Calcium signaling protocols
Publication
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Confocal Microscopy: Theory and Applications for Cellular Signaling -- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer -- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®) -- Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader -- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones -- Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells -- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells -- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Calcium Measurements from Whole Heart Using Rhod-2.- Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries -- Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes -- Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors -- Compartmentalizing Genetically-Encoded Calcium Sensors -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Control code
990475994
Dimensions
unknown
Edition
3rd ed. 2013.
Extent
1 online resource (XI, 360 pages 51 illustrations, 17 illustrations in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627030854
Lccn
2012946739
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-086-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)990475994

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...