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The Resource Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors, edited by Antonio Giordano, Gaetano Romano

Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors, edited by Antonio Giordano, Gaetano Romano

Contributor
Summary
The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies
Language
eng
Work
Publication
Extent
1 online resource (xiii, 185 pages)
Contents
  • The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed [and others] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed [and others] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed [and others] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed [and others] -- Chromatin immunoprecipitation / Amjad Javed [and others]
  • Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed [and others] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell
  • The transformed phenotype / Henry Hoff [and others] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation [and others] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino [and others] -- Induction of deoxyribonucleic acid damage by -- irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini [and others]
  • Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and '-galactoside staining / Tiziana Tonini [and others] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini [and others] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini [and others] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio [and others]
Isbn
9780896039490
Instance
Label
Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors
Title
Cell cycle control and dysregulation protocols
Title remainder
cyclins, cyclin-dependent kinases, and other factors
Statement of responsibility
edited by Antonio Giordano, Gaetano Romano
Contributor
Subject
Genre
Language
eng
Summary
The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies
Member of
Cataloging source
COO
Dewey number
571.8/4
Illustrations
illustrations
Index
index present
LC call number
QH605
LC item number
.C4257 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 55
NLM item number
  • ME9616J v.285 2004
  • C39265 2004
http://library.link/vocab/relatedWorkOrContributorDate
1959-
http://library.link/vocab/relatedWorkOrContributorName
  • Giordano, Antonio
  • Romano, Gaetano
Series statement
Methods in molecular biology,
Series volume
285
http://library.link/vocab/subjectName
  • Cell cycle
  • Cyclin-dependent kinases
  • Cyclins
  • Biology
  • Immunohistochemistry
  • Nucleic Acids
  • Protein-Serine-Threonine Kinases
  • Genetic Phenomena
  • Proline-Directed Protein Kinases
  • Proteins
  • Peptides
  • Immunologic Tests
  • Amino Acids, Peptides, and Proteins
  • Biological Science Disciplines
  • Protein Kinases
  • Histocytochemistry
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Natural Science Disciplines
  • Immunologic Techniques
  • Phenomena and Processes
  • Histological Techniques
  • Clinical Laboratory Techniques
  • Phosphotransferases (Alcohol Group Acceptor)
  • Diagnosis
  • Phosphotransferases
  • Investigative Techniques
  • Disciplines and Occupations
  • Transferases
  • Enzymes
  • Enzymes and Coenzymes
  • Cell Cycle Proteins
  • DNA
  • Gene Expression
  • Fluorescent Antibody Technique
  • Cyclin-Dependent Kinases
  • Intracellular Signaling Peptides and Proteins
  • Genetics
  • SCIENCE
  • Cell cycle
  • Cyclin-dependent kinases
  • Cyclins
Label
Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors, edited by Antonio Giordano, Gaetano Romano
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed [and others] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed [and others] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed [and others] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed [and others] -- Chromatin immunoprecipitation / Amjad Javed [and others]
  • Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed [and others] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell
  • The transformed phenotype / Henry Hoff [and others] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation [and others] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino [and others] -- Induction of deoxyribonucleic acid damage by -- irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini [and others]
  • Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and '-galactoside staining / Tiziana Tonini [and others] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini [and others] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini [and others] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio [and others]
Control code
56471028
Dimensions
unknown
Extent
1 online resource (xiii, 185 pages)
Form of item
online
Isbn
9780896039490
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)56471028
Label
Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors, edited by Antonio Giordano, Gaetano Romano
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed [and others] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed [and others] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed [and others] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed [and others] -- Chromatin immunoprecipitation / Amjad Javed [and others]
  • Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed [and others] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell
  • The transformed phenotype / Henry Hoff [and others] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation [and others] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino [and others] -- Induction of deoxyribonucleic acid damage by -- irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini [and others]
  • Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and '-galactoside staining / Tiziana Tonini [and others] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini [and others] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini [and others] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio [and others]
Control code
56471028
Dimensions
unknown
Extent
1 online resource (xiii, 185 pages)
Form of item
online
Isbn
9780896039490
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)56471028

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