The Resource Characterization of membrane viscosity changes with the novel molecular rotor FCVJ, by Matthew E. Nipper, (electronic resource)

Characterization of membrane viscosity changes with the novel molecular rotor FCVJ, by Matthew E. Nipper, (electronic resource)

Label
Characterization of membrane viscosity changes with the novel molecular rotor FCVJ
Title
Characterization of membrane viscosity changes with the novel molecular rotor FCVJ
Statement of responsibility
by Matthew E. Nipper
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Membrane viscosity can be defined as the "thickness" of a cell membrane. Membrane viscosity conditions are a useful indicator of cell health. Many diseases have been reported to induce changes in afflicted cell membrane viscosity. Characterizing membrane viscosity will provide researchers and clinicians with a valuable tool in diagnosing the onset and progression of diseases. Molecular rotors are fluorescent molecules that have been shown to exhibit viscosity sensitive DLPC liposomes were formed with the molecular rotor FCVJ incorporated into the membrane. A cuvette with liposomes was excited at 460 nm under fluorescent spectroscopy and intensity values were recorded. The procedure was repeated for a 2% cyclohexane/sucrose solution. Peak emissions were compared and the cyclohexane fluidized the membrane resulting in a statistically significant reduction in intensity. To achieve a converse effect, a 20% (v/v) Cholesterol/DLPC mixture was used to produce liposomes in the presence of sucrose solution. Intensity values were compared to those of the control group for the cyclohexane experiments. The intensity values for the cholesterol group were higher than control. The liposomes used in this experiment are a suitable model for the mammalian cell membrane in both size and physical similarities. This previously undocumented method for characterizing membrane conditions provides a qualitative method of measuring changes in membrane viscosity
Cataloging source
MUU
http://library.link/vocab/creatorName
Nipper, Matthew Edward
Degree
M.S.
Dissertation year
2007.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Haidekker, Mark A.
http://library.link/vocab/subjectName
  • Viscosity
  • Membranes (Biology)
  • Cell interaction
Target audience
specialized
Label
Characterization of membrane viscosity changes with the novel molecular rotor FCVJ, by Matthew E. Nipper, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on May 12, 2009)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
320835608
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)320835608
System details
Mode of access: World Wide Web
Label
Characterization of membrane viscosity changes with the novel molecular rotor FCVJ, by Matthew E. Nipper, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on May 12, 2009)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
320835608
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)320835608
System details
Mode of access: World Wide Web

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