The Resource Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride, by Jason William Frank

Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride, by Jason William Frank

Label
Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride
Title
Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride
Statement of responsibility
by Jason William Frank
Creator
Subject
Language
eng
Summary
This series of studies were designed to evaluate the acute-phase response in pigs following an immunological challenge with lipopolysaccharide (LPS). The first experiment utilized six pigs (initial BW = 62.6 ± 1.3 kg) that underwent repeated injection of LPS at 15 [mu]g/kg BW every other day for a period of nine days. Pigs became tolerant to the LPS challenge following the third injection as indicated by the attenuation of the suppressions in feed intake. Following the initial LPS challenge, one of pig died between 12 and 24 h and the other pig failed to consume any feed for a period of 3 d. To investigate this hypersensitivity, 192 pigs (initial BW = 39.9 ± 3.6 kg) were injected with 15 [mu]g/kg BW LPS and changes in feed intake were measure over a 48 h period. This response is defined as the acute feed intake response (AFIR) and is calculated as the ADFI from 0 to 48 h divided by the ADFI from minus 48 to 0 h relative to the LPS challenge. This value is then multiplied by 100%. The mean AFIR for these pigs was 54.7 ± 17.4%. This data was normally distributed and those pigs have an AFIR less than or greater than two times the standard deviation from the mean were considered hyper-responsive and hypo-responsive, respectively. Based on these observations, the used of the AFIR of pigs following an initial immune challenge with LPS can be used to identify hyper- and hypo-responsive pigs. The second experiment evaluated the AFIR, serum profiles of cortisol and tumor necrosis factor-[alpha] (TNF-[alpha]), and the subsequent growth performance of two genotypes (Line A and B) of pigs following an LPS challenge. No consistent genotype differences were observed in AFIR or serum profiles of cortisol and TNF-[alpha]. Pigs were categorized based on AFIR to evaluate subsequent growth performance, however, no differences were associated with AFIR categorization. Line A pigs tended to have greater average daily gain (P < 0.10) and greater gain-to-feed ratios (P < 0.01) than Line B pigs. The data from the second experiment indicated no differences were ascertainable in the immune response of these two genotypes of pigs. The final experiment was designed to evaluate the AFIR and acute-phase protein (APP) responses of two genotypes and genders of pigs (initial BW = 21.3 ± 0.48 kg) to three levels of LPS (0, 25, or 50 [mu]g/kg BW). Measures of AFIR decreased quadratically with increasing LPS dose (P < 0.05). No genotype differences were observed in AFIR. Three barrows and no gilts died following the LPS challenge (P < 0.10). Barrows also had higher d 0 haptoglobin (HPT; P < 0.03) and greater increases in ceruloplasmin (Cp; P < 0.05) concentrations than gilts. No effect of LPS treatment was observed in serum C-reactive protein, Cp, or HPT concentrations (P > 0.10). Serum amyloid A (SAA) concentrations increased quadratically with increasing LPS dose (P < 0.02). The results of this study indicate barrows may be more sensitive than gilts to an LPS challenge and SAA appears to be a viable biomarker of immune system activation in pigs. Overall, these experiments indicate measuring acute changes in feed intake may serve as an accurate means of evaluating the acute phase response in pigs. In addition, no differences in immune system activation were observed between the genotypes evaluated in these experiments
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1974-
http://library.link/vocab/creatorName
Frank, Jason W.
Degree
Ph. D.
Dissertation year
2003.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Swine
  • Swine
  • Immune response
  • Endotoxins
Target audience
specialized
Label
Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride, by Jason William Frank
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 94-101)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
53987542
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 102 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Label
Characterization of the feed intake and acute-phase protein responses of pigs following an acute immune challenge with lipopolysaccaride, by Jason William Frank
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 94-101)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
53987542
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 102 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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