The Resource Cloning and sequence analysis of multiple genes from Bifidobacterium infantis, by Liwen Tu

Cloning and sequence analysis of multiple genes from Bifidobacterium infantis, by Liwen Tu

Label
Cloning and sequence analysis of multiple genes from Bifidobacterium infantis
Title
Cloning and sequence analysis of multiple genes from Bifidobacterium infantis
Statement of responsibility
by Liwen Tu
Creator
Subject
Language
eng
Summary
Bifidobacteria are probiotics that are commonly added in a multitude of dairy products. However, there is a lack of knowledge of their genes. Investigations into the multiple genes in this important organism will contribute to our understanding and ability to manipulate it for future important commercial application purposes. Our objectives were to clone and sequence multiple genes from Bifidobacterium infantis . A genomic DNA library of B. infantis 15702 was constructed in [lambda] phage and 5 positive clones were further analyzed. Sequencing was performed by primer walking from both of the 5 ' and 3 ' directions of the cloned DNA. Sequence results revealed the presence of six open reading frames (ORF) on the cloned fragments. The six ORF had the highest identities with the epimerase of Streptococcus mutans (60.8%), [beta]-galactosidase of Leuconostoc lactis (strain NZ6009) plasmid pNZ63 (62.5%), lipase of Deinococcus radiodurans (strain R1) (34.2%), 50S ribosomal proteins L9 of section 6 of 114 of the complete genome of Rickettsi conorii Malish 7 (59.9%), transposase of Deinococcus radiodurans (strain R1) (35.0%), and aminoglycoside N3-acetyltransferases of Nostoc sp. (strain PCC 7120) (39.1%), respectively. The structure of the bifidobacterial genes will provide the basis for genetic approaches that will lead to potential developments of commercial strains with improved characteristics
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1970-
http://library.link/vocab/creatorName
Tu, Liwen
Degree
Ph. D.
Dissertation year
2004.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Bifidobacterium
  • Molecular cloning
  • Molecular genetics
Target audience
specialized
Label
Cloning and sequence analysis of multiple genes from Bifidobacterium infantis, by Liwen Tu
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 117-130)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
57480070
Dimensions
29 cm
Dimensions
unknown
Extent
x, 131 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
Label
Cloning and sequence analysis of multiple genes from Bifidobacterium infantis, by Liwen Tu
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 117-130)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
57480070
Dimensions
29 cm
Dimensions
unknown
Extent
x, 131 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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