Coverart for item
The Resource Cytotoxic T-cells : methods and protocols, edited by Elena Ranieri

Cytotoxic T-cells : methods and protocols, edited by Elena Ranieri

Label
Cytotoxic T-cells : methods and protocols
Title
Cytotoxic T-cells
Title remainder
methods and protocols
Statement of responsibility
edited by Elena Ranieri
Contributor
Editor
Subject
Genre
Language
eng
Summary
"In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--Publisher's description
Member of
Cataloging source
GW5XE
Dewey number
571.9/66
Illustrations
illustrations
Index
index present
LC call number
QR185.8.T2
LC item number
C9877 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • WH 200
NLM item number
ME9616J v.1186 2014
http://library.link/vocab/relatedWorkOrContributorName
Ranieri, Elena
Series statement
Methods in Molecular Biology,
Series volume
Volume 1186
http://library.link/vocab/subjectName
  • T cells
  • Systems biology
  • T-Lymphocytes, Cytotoxic
  • Systems Biology
  • T cells
  • Systems biology
  • Biology
Label
Cytotoxic T-cells : methods and protocols, edited by Elena Ranieri
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Iulia Popescu, Matthew Pipeling, Jason Akulian, and John McDyer
  • 4.
  • Intracellular Signaling of CTLs
  • Sharon Natasha Cox
  • 5.
  • Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
  • Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus
  • 6.
  • CTL ELISPOT Assay
  • Elena Ranieri, Iulia Popescu, and Margherita Gigante
  • 1.
  • 7.
  • Methods of Purification of CTL-Derived Exosomes
  • Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
  • 8.
  • Characterization of CTL by Microscopy
  • Giuseppe Stefano Netti
  • 9.
  • Intravital Imaging of Cytotoxic T Lymphocytes
  • Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi
  • 10.
  • Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8 T Cells from Blood and Tumor Tissue
  • The Microarray-Based Approach for the Analysis of the Transcriptome
  • Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo
  • 11.
  • miRNome Analysis Using Real-Time PCR
  • Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo
  • 12.
  • Proteomic Approaches by SELDI and MALDI-TOF/MS for CTL Analysis
  • Massimo Papale and Maria Teresa Rocchetti
  • 13.
  • Two-Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis
  • Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri
  • Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo
  • 14.
  • Targeting the JAK/STAT Pathway in Cytotoxic T lymphocytes (CTL) by Next Generation Sequencing (NGS)
  • Maddalena Gigante, Sterpeta Diella, and Elena Ranieri
  • 15.
  • Experimental Model for Studying the Involvement of Regulatory Cytotoxic T Cells in Bone Resorption
  • Giacomina Brunetti, Angela Oranger, Silvia Colucci, and Maria Grano
  • 16.
  • CTL and Transplantation: Tissue In Vivo Characterization
  • Federica Rascio, Chiara Divella, and Giuseppe Grandaliano
  • 2.
  • In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes
  • Margherita Gigante and Elena Ranieri
  • 3.
  • Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry
Control code
889943787
Dimensions
unknown
Extent
1 online resource (xii, 301 pages)
File format
unknown
Form of item
online
Isbn
9781493911585
Lccn
2014944379
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1158-5
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)889943787
Label
Cytotoxic T-cells : methods and protocols, edited by Elena Ranieri
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Iulia Popescu, Matthew Pipeling, Jason Akulian, and John McDyer
  • 4.
  • Intracellular Signaling of CTLs
  • Sharon Natasha Cox
  • 5.
  • Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR
  • Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus
  • 6.
  • CTL ELISPOT Assay
  • Elena Ranieri, Iulia Popescu, and Margherita Gigante
  • 1.
  • 7.
  • Methods of Purification of CTL-Derived Exosomes
  • Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
  • 8.
  • Characterization of CTL by Microscopy
  • Giuseppe Stefano Netti
  • 9.
  • Intravital Imaging of Cytotoxic T Lymphocytes
  • Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi
  • 10.
  • Negative and Positive Separation Techniques for the Isolation of Antigen-Specific CD8 T Cells from Blood and Tumor Tissue
  • The Microarray-Based Approach for the Analysis of the Transcriptome
  • Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo
  • 11.
  • miRNome Analysis Using Real-Time PCR
  • Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo
  • 12.
  • Proteomic Approaches by SELDI and MALDI-TOF/MS for CTL Analysis
  • Massimo Papale and Maria Teresa Rocchetti
  • 13.
  • Two-Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis
  • Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri
  • Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo
  • 14.
  • Targeting the JAK/STAT Pathway in Cytotoxic T lymphocytes (CTL) by Next Generation Sequencing (NGS)
  • Maddalena Gigante, Sterpeta Diella, and Elena Ranieri
  • 15.
  • Experimental Model for Studying the Involvement of Regulatory Cytotoxic T Cells in Bone Resorption
  • Giacomina Brunetti, Angela Oranger, Silvia Colucci, and Maria Grano
  • 16.
  • CTL and Transplantation: Tissue In Vivo Characterization
  • Federica Rascio, Chiara Divella, and Giuseppe Grandaliano
  • 2.
  • In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes
  • Margherita Gigante and Elena Ranieri
  • 3.
  • Phenotypic and Functional Characterization of Cytotoxic T Lymphocytes by Flow Cytometry
Control code
889943787
Dimensions
unknown
Extent
1 online resource (xii, 301 pages)
File format
unknown
Form of item
online
Isbn
9781493911585
Lccn
2014944379
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1158-5
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)889943787

Library Locations

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      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
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