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The Resource DNA damage detection in situ, ex vivo, and in vivo : methods and protocols, edited by Vladimir V. Didenko

DNA damage detection in situ, ex vivo, and in vivo : methods and protocols, edited by Vladimir V. Didenko

Label
DNA damage detection in situ, ex vivo, and in vivo : methods and protocols
Title
DNA damage detection in situ, ex vivo, and in vivo
Title remainder
methods and protocols
Statement of responsibility
edited by Vladimir V. Didenko
Contributor
Subject
Genre
Language
eng
Summary
Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology{u2122} series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science
Member of
Cataloging source
SCB
Dewey number
572.86
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QH465
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 477
http://library.link/vocab/relatedWorkOrContributorName
Didenko, Vladimir V
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
v. 682
http://library.link/vocab/subjectName
  • DNA damage
  • DNA damage
  • DNA
  • Genetic Phenomena
  • Clinical Laboratory Techniques
  • Publication Formats
  • Nucleic Acids
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Publication Characteristics
  • Investigative Techniques
  • DNA Damage
  • DNA
  • Microbiological Techniques
  • Laboratory Manuals
  • Phenomena and Processes
  • DNA
  • DNA damage
Label
DNA damage detection in situ, ex vivo, and in vivo : methods and protocols, edited by Vladimir V. Didenko
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
In situ detection of apoptosis by the TUNEL assay: An overview of techniques -- Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage -- EM-ISEL: A useful tool to visualize DNA damage at the ultrastructural level -- In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase -- In situ ligation: A decade and a half of experience -- In situ ligation simplified: Using PCR fragments for detection of double-strand DNA breaks in tissue sections -- 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach -- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry -- Fluorochrome-labeled inhibitors of caspases: Convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis -- Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair -- Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) -- Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells -- Ultrasound imaging of apoptosis: Spectroscopic detection of DNA-damage effects at high and low frequencies -- Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)P-postlabeling and immunohistochemistry -- ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells -- Cytokinesis-block micronucleus cytome assay in lymphocytes -- Buccal micronucleus cytome assay -- [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin -- Immunologic detection of benzo(a)pyrene-DNA adducts -- Non-invasive assessment of oxidatively damaged DNA: Liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine -- Assessing sperm DNA fragmentation with the sperm chromatin dispersion test
Control code
681767573
Extent
1 online resource (xiii, 313 pages)
Form of item
online
Isbn
9781603274098
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-60327-409-8
Other physical details
illustrations (some color).
http://library.link/vocab/ext/overdrive/overdriveId
978-1-60327-408-1
Specific material designation
remote
System control number
(OCoLC)681767573
Label
DNA damage detection in situ, ex vivo, and in vivo : methods and protocols, edited by Vladimir V. Didenko
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
In situ detection of apoptosis by the TUNEL assay: An overview of techniques -- Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage -- EM-ISEL: A useful tool to visualize DNA damage at the ultrastructural level -- In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase -- In situ ligation: A decade and a half of experience -- In situ ligation simplified: Using PCR fragments for detection of double-strand DNA breaks in tissue sections -- 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach -- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry -- Fluorochrome-labeled inhibitors of caspases: Convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis -- Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair -- Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) -- Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells -- Ultrasound imaging of apoptosis: Spectroscopic detection of DNA-damage effects at high and low frequencies -- Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)P-postlabeling and immunohistochemistry -- ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells -- Cytokinesis-block micronucleus cytome assay in lymphocytes -- Buccal micronucleus cytome assay -- [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin -- Immunologic detection of benzo(a)pyrene-DNA adducts -- Non-invasive assessment of oxidatively damaged DNA: Liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine -- Assessing sperm DNA fragmentation with the sperm chromatin dispersion test
Control code
681767573
Extent
1 online resource (xiii, 313 pages)
Form of item
online
Isbn
9781603274098
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-60327-409-8
Other physical details
illustrations (some color).
http://library.link/vocab/ext/overdrive/overdriveId
978-1-60327-408-1
Specific material designation
remote
System control number
(OCoLC)681767573

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