Coverart for item
The Resource DNA repair, edited by Judith Campbell, Paul Modrich

DNA repair, edited by Judith Campbell, Paul Modrich

Label
DNA repair
Title
DNA repair
Statement of responsibility
edited by Judith Campbell, Paul Modrich
Contributor
Editor
Subject
Language
eng
Summary
This volume emphasizes the intracellular consequences of DNA damage, describing procedures for analysis of checkpoint responses, DNA repair in vivo, replication fork encounter of DNA damage, as well as biological methods for analysis of mutation production and chromosome rearrangements. It also describes molecular methods for analysis of a number of genome maintenance activities including DNA ligases, helicases, and single-strand binding proteins. *Part B of a 2-part series *Addresses DNA maintenance enzymes *Discusses damage signaling *Presents In vivo analysis of DNA repair *Covers mutation and chromosome rearrangements
Member of
Cataloging source
YUM
Dewey number
572.86459
Illustrations
  • illustrations
  • plates
Index
index present
LC call number
  • QP601
  • QH467
LC item number
  • .C57 v. 408-409
  • .D572 2006
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 475
NLM item number
D6284 2006
http://library.link/vocab/relatedWorkOrContributorName
  • Campbell, Judith L.
  • Modrich, Paul
Series statement
Methods in enzymology,
Series volume
v. 408, 409
http://library.link/vocab/subjectName
  • DNA repair
  • Enzymology
  • DNA Repair
  • Enzymes
  • DNA repair
  • Enzymology
Label
DNA repair, edited by Judith Campbell, Paul Modrich
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Part A (v. 408) -- Part B (v. 409)
  • Cover -- Cover Page -- Table of Contents -- Contributors of Volume 408 -- Volumes in Series -- Chapter 1: Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site-Specific DNA Lesions In Vivo -- Introduction -- Genome Construction -- Genome Normalization -- Lesion Bypass (CRAB) Assay -- Lesion Mutagenesis (REAP) Assay -- Conclusion -- References -- Chapter 2: Oxidative DNA Glycosylases: Recipes from Cloning to Characterization -- Introduction -- Purification of DNA Glycosylases -- Preparation of Oligonucleotide Substrates -- Activity Assays for Oxidative DNA Glycosylases -- Acknowledgment -- References -- Chapter 3: Purification and Characterization of NEIL1 and NEIL2, Members of a Distinct Family of Mammalian DNA Glycosylases for Repair of Oxidized Bases -- Endogenous and Induced DNA Damage -- Repair of Oxidized Base Lesions in the Genome -- Two Classes of Oxidized Base-Specific Glycosylases -- Human Genome Database Analysis and Discovery of MutM/Nei-Like Proteins -- Enzymatic Activity of NEIL1 and NEIL2 with Duplex or Bubble Oligonucleotide Substrates -- Detection of DNA Glycosylase Activity of Recombinant NEILs by Trapping Analysis -- Acknowledgments -- References -- Chapter 4: Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-Links -- Introduction -- Preparation of dL-DNA Substrates -- Analysis of dL-mediated Spontaneous DNA-Protein Cross-Links -- Analysis of dL-Specific BER -- Acknowledgments -- References -- Chapter 5: Isolation and Analyses of MutY Homologs (MYH) -- Introduction -- Isolation of MutY and MYH Proteins -- Functional Assays of MutY and MYH -- Protein Interaction Assays -- Acknowledgments -- References -- Chapter 6: Use of Yeast for Detection of Endogenous Abasic Lesions, Their Source, and Their Repair -- AP Sites in DNA and Related 3'-Blocked and 5'-Blocked Single Strand Breaks Form an Abundant and Deleterious Class of Endogenous DNA Damage -- Endogenous AP Sites Cause Cell Death in the Absence of Apn1, Apn2, and Rad1-Rad10 -- Use of the apn1 apn2 rad1 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA -- Use of the apn1 apn2 rad14 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA -- Conclusions and Model -- Acknowledgments -- References -- Chapter 7: Activities and Mechanism of DNA Polymerase beta -- Introduction -- Kinetic Characteristics of DNA Synthesis -- Measurement of dRP Lyase Activity -- Measurement of In Vitro BER -- Reagents -- Acknowledgment -- References -- Chapter 8: Direct Removal of Alkylation Damage from DNA by AlkB and Related DNA Dioxygenases -- Purification of AlkB, ABH2, and ABH3 -- 14C-Methylated Substrates -- Assays -- In Vivo Assay: Survival of Alkylated Single-Stranded DNA Bacteriophage in E. coli AlkB Mutants -- References -- Chapter 9: Purification and Characterization of DNA Photolyases -- Introduction -- Overexpression and Purification of Photolyases -- Spectroscopic Properties and Characterization of Purified Photolyases
Control code
70841301
Dimensions
unknown
Extent
1 online resource (parts A-B)
File format
unknown
Form of item
online
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)70841301
Label
DNA repair, edited by Judith Campbell, Paul Modrich
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Part A (v. 408) -- Part B (v. 409)
  • Cover -- Cover Page -- Table of Contents -- Contributors of Volume 408 -- Volumes in Series -- Chapter 1: Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site-Specific DNA Lesions In Vivo -- Introduction -- Genome Construction -- Genome Normalization -- Lesion Bypass (CRAB) Assay -- Lesion Mutagenesis (REAP) Assay -- Conclusion -- References -- Chapter 2: Oxidative DNA Glycosylases: Recipes from Cloning to Characterization -- Introduction -- Purification of DNA Glycosylases -- Preparation of Oligonucleotide Substrates -- Activity Assays for Oxidative DNA Glycosylases -- Acknowledgment -- References -- Chapter 3: Purification and Characterization of NEIL1 and NEIL2, Members of a Distinct Family of Mammalian DNA Glycosylases for Repair of Oxidized Bases -- Endogenous and Induced DNA Damage -- Repair of Oxidized Base Lesions in the Genome -- Two Classes of Oxidized Base-Specific Glycosylases -- Human Genome Database Analysis and Discovery of MutM/Nei-Like Proteins -- Enzymatic Activity of NEIL1 and NEIL2 with Duplex or Bubble Oligonucleotide Substrates -- Detection of DNA Glycosylase Activity of Recombinant NEILs by Trapping Analysis -- Acknowledgments -- References -- Chapter 4: Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-Links -- Introduction -- Preparation of dL-DNA Substrates -- Analysis of dL-mediated Spontaneous DNA-Protein Cross-Links -- Analysis of dL-Specific BER -- Acknowledgments -- References -- Chapter 5: Isolation and Analyses of MutY Homologs (MYH) -- Introduction -- Isolation of MutY and MYH Proteins -- Functional Assays of MutY and MYH -- Protein Interaction Assays -- Acknowledgments -- References -- Chapter 6: Use of Yeast for Detection of Endogenous Abasic Lesions, Their Source, and Their Repair -- AP Sites in DNA and Related 3'-Blocked and 5'-Blocked Single Strand Breaks Form an Abundant and Deleterious Class of Endogenous DNA Damage -- Endogenous AP Sites Cause Cell Death in the Absence of Apn1, Apn2, and Rad1-Rad10 -- Use of the apn1 apn2 rad1 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA -- Use of the apn1 apn2 rad14 Triple Mutant to Study the Origin and Repair of Endogenous AP Sites in DNA -- Conclusions and Model -- Acknowledgments -- References -- Chapter 7: Activities and Mechanism of DNA Polymerase beta -- Introduction -- Kinetic Characteristics of DNA Synthesis -- Measurement of dRP Lyase Activity -- Measurement of In Vitro BER -- Reagents -- Acknowledgment -- References -- Chapter 8: Direct Removal of Alkylation Damage from DNA by AlkB and Related DNA Dioxygenases -- Purification of AlkB, ABH2, and ABH3 -- 14C-Methylated Substrates -- Assays -- In Vivo Assay: Survival of Alkylated Single-Stranded DNA Bacteriophage in E. coli AlkB Mutants -- References -- Chapter 9: Purification and Characterization of DNA Photolyases -- Introduction -- Overexpression and Purification of Photolyases -- Spectroscopic Properties and Characterization of Purified Photolyases
Control code
70841301
Dimensions
unknown
Extent
1 online resource (parts A-B)
File format
unknown
Form of item
online
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)70841301

Library Locations

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      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
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