Coverart for item
The Resource DNA repair protocols : mammalian systems, edited by Daryl S. Henderson

DNA repair protocols : mammalian systems, edited by Daryl S. Henderson

Label
DNA repair protocols : mammalian systems
Title
DNA repair protocols
Title remainder
mammalian systems
Statement of responsibility
edited by Daryl S. Henderson
Contributor
Subject
Genre
Language
eng
Summary
In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair
Member of
Cataloging source
COO
Dewey number
572.8/6
Illustrations
illustrations
Index
index present
LC call number
QH467
LC item number
.D627 2005
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 314
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.314 2006
  • D755 2006
http://library.link/vocab/relatedWorkOrContributorName
Henderson, Daryl S
Series statement
Methods in molecular biology
Series volume
314
http://library.link/vocab/subjectName
  • DNA repair
  • Mammals
  • DNA Repair
  • DNA Damage
  • SCIENCE
  • DNA repair
  • DNA
  • Herstel
  • Eukaryoten
  • Wetenschappelijke technieken
  • Dna
  • Reparação de dna
  • Mamíferos (genética)
Label
DNA repair protocols : mammalian systems, edited by Daryl S. Henderson
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka -- Complementation assays adapted for DNA repair/deficient keratinocytes / Mathilde Fréchet [and others] -- Cytogenetic challenge assays for assessment of DNA repair capacities / William Au and Salama A. Salama -- Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan -- Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang
  • Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz -- Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi -- In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong -- Detection of DNA double-strand breaks and chromosomal translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Plasmid-based assays for DNA end joining in vitro / George Iliakis [and others] -- Use of gene targeting to study recombination in mammalian DNA repair mutants / Rodney Nairn and Gerald Adair
  • Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr -- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos [and others] -- Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer -- Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson -- A dot-blot immunoassay for measuring repair of UV photoproducts / Shirley McCready -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell -- DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland
  • The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Hartmann -- Fast micromethod DNA single-strand break assay / Heinz C. Schröder [and others] -- 32P-postlabeling DNA-damage assays: PAGE, TLC, and HPLC / Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki -- Electrophoretic mobility shift assays to study protein binding to damaged DNA / Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- Construction of MMR plasmid substrates and analysis of MMR error correction and excision / Huixian Wang and John B. Hays -- Analysis of enzymes that act at abasic sites in DNA / Walter A. Deutsch and Vijay Hegde -- Base excision repair in mammalian cells / Yoshihiro Matsumoto -- In vitro base excision repair assay using mammalian cell extracts / Guido Frosina [and others]
  • Biochemical assays for the characterization of DNA helicases / Robert M. Brosh Jr. and Sudha Sharma -- Repair synthesis assay for nucleotide excision repair activity using fractionated cell extracts and UV-damaged plasmid DNA / Maureen Biggerstaff and Richard D. Wood -- Assaying for the dual incisions of nucleotide excision repair using DNA with a lesion at a specific site / Mahmud K.K. Shivji [and others] -- Analysis of proliferating cell nuclear antigen (PCNA) associated with DNA excision repair sites in mammalian cells / A. Ivana Scovassi and Ennio Prosperi -- Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni
Control code
209947717
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvii, 498 pages)
Form of item
online
Isbn
9781588295132
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-513-2
Specific material designation
remote
System control number
(OCoLC)209947717
Label
DNA repair protocols : mammalian systems, edited by Daryl S. Henderson
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka -- Complementation assays adapted for DNA repair/deficient keratinocytes / Mathilde Fréchet [and others] -- Cytogenetic challenge assays for assessment of DNA repair capacities / William Au and Salama A. Salama -- Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan -- Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang
  • Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz -- Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi -- In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong -- Detection of DNA double-strand breaks and chromosomal translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Plasmid-based assays for DNA end joining in vitro / George Iliakis [and others] -- Use of gene targeting to study recombination in mammalian DNA repair mutants / Rodney Nairn and Gerald Adair
  • Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr -- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos [and others] -- Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer -- Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson -- A dot-blot immunoassay for measuring repair of UV photoproducts / Shirley McCready -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell -- DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland
  • The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Hartmann -- Fast micromethod DNA single-strand break assay / Heinz C. Schröder [and others] -- 32P-postlabeling DNA-damage assays: PAGE, TLC, and HPLC / Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki -- Electrophoretic mobility shift assays to study protein binding to damaged DNA / Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- Construction of MMR plasmid substrates and analysis of MMR error correction and excision / Huixian Wang and John B. Hays -- Analysis of enzymes that act at abasic sites in DNA / Walter A. Deutsch and Vijay Hegde -- Base excision repair in mammalian cells / Yoshihiro Matsumoto -- In vitro base excision repair assay using mammalian cell extracts / Guido Frosina [and others]
  • Biochemical assays for the characterization of DNA helicases / Robert M. Brosh Jr. and Sudha Sharma -- Repair synthesis assay for nucleotide excision repair activity using fractionated cell extracts and UV-damaged plasmid DNA / Maureen Biggerstaff and Richard D. Wood -- Assaying for the dual incisions of nucleotide excision repair using DNA with a lesion at a specific site / Mahmud K.K. Shivji [and others] -- Analysis of proliferating cell nuclear antigen (PCNA) associated with DNA excision repair sites in mammalian cells / A. Ivana Scovassi and Ennio Prosperi -- Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni
Control code
209947717
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvii, 498 pages)
Form of item
online
Isbn
9781588295132
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-513-2
Specific material designation
remote
System control number
(OCoLC)209947717

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