Coverart for item
The Resource DNA repair protocols : mammalian systems, edited by Daryl S. Henderson

DNA repair protocols : mammalian systems, edited by Daryl S. Henderson

Label
DNA repair protocols : mammalian systems
Title
DNA repair protocols
Title remainder
mammalian systems
Statement of responsibility
edited by Daryl S. Henderson
Contributor
Subject
Genre
Language
eng
Summary
This collection of readily reproducible techniques for repairing mammalian DNA contains fourteen entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of adducted bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting
Member of
Cataloging source
DNLM/DLC
Dewey number
572.8/6
Illustrations
illustrations
Index
index present
LC call number
QH467
LC item number
.D627 2006
Literary form
non fiction
Nature of contents
bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.314 2006
  • D755 2006
http://library.link/vocab/relatedWorkOrContributorName
Henderson, Daryl S
Series statement
Methods in molecular biology
Series volume
314
http://library.link/vocab/subjectName
  • DNA repair
  • DNA Repair
  • DNA Damage
  • Mammals
Label
DNA repair protocols : mammalian systems, edited by Daryl S. Henderson
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka -- Complementation assays adapted for DNA repair-deficient keratinocytes / Mathilde Fréchet ... [et al.] -- Cytogenetic challenge assays for assessment of DNA repair capacities / William W. Au and Salama A. Salama -- Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan -- Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the SV40-based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang --
  • Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz -- Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafuji -- In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong -- Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Plasmid-based assays for DNA end-joining in vitro / George Iliakis ... [et al.] -- Use of gene targeting to study recombination in mammalian cell DNA repair mutants / Rodney S. Nairn and Gerald M. Adair --
  • Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr -- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos ... [et al.] -- Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer -- Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson -- A dot-blot immunoassay for measuring repair of ultraviolet photoproducts / Shirley McCready -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell -- DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland --
Control code
58431712
Dimensions
24 cm
Edition
2nd ed.
Extent
xvii, 498 pages
Isbn
9781588295132
Isbn Type
(alk. paper)
Lccn
2005006250
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
Label
DNA repair protocols : mammalian systems, edited by Daryl S. Henderson
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka -- Complementation assays adapted for DNA repair-deficient keratinocytes / Mathilde Fréchet ... [et al.] -- Cytogenetic challenge assays for assessment of DNA repair capacities / William W. Au and Salama A. Salama -- Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan -- Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka -- Analysis of inhibition of DNA replication in irradiated cells using the SV40-based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang --
  • Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz -- Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafuji -- In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong -- Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Plasmid-based assays for DNA end-joining in vitro / George Iliakis ... [et al.] -- Use of gene targeting to study recombination in mammalian cell DNA repair mutants / Rodney S. Nairn and Gerald M. Adair --
  • Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr -- Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos ... [et al.] -- Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer -- Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson -- A dot-blot immunoassay for measuring repair of ultraviolet photoproducts / Shirley McCready -- Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell -- DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland --
Control code
58431712
Dimensions
24 cm
Edition
2nd ed.
Extent
xvii, 498 pages
Isbn
9781588295132
Isbn Type
(alk. paper)
Lccn
2005006250
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...