Coverart for item
The Resource DNA repair protocols : prokaryotic systems, edited by Pat Vaughan

DNA repair protocols : prokaryotic systems, edited by Pat Vaughan

Label
DNA repair protocols : prokaryotic systems
Title
DNA repair protocols
Title remainder
prokaryotic systems
Statement of responsibility
edited by Pat Vaughan
Contributor
Subject
Genre
Language
eng
Summary
DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology
Member of
Cataloging source
N$T
Dewey number
572.8/6459293
Illustrations
illustrations
Index
index present
LC call number
QH467
LC item number
.D63 2000eb
Literary form
non fiction
NAL call number
QH467
NAL item number
.D63 2000eb
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.152 2000
  • D62895 2000
http://library.link/vocab/relatedWorkOrContributorDate
1967-
http://library.link/vocab/relatedWorkOrContributorName
Vaughan, Pat
Series statement
Methods in molecular biology
Series volume
152
http://library.link/vocab/subjectName
  • DNA repair
  • DNA Repair
  • Prokoryotic Cells
  • SCIENCE
  • DNA repair
Label
DNA repair protocols : prokaryotic systems, edited by Pat Vaughan
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Detection and quanitation of recBCD enzymer (exonuclease V) activity
  • Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima
  • MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance
  • pt. 2. Use of DNA repair enzymes as tools in molecular biology
  • Flexible genetic engineering using RecA protein
  • Interspecies recombination and mismatch repair: generation of mosaic genes and genomes
  • The use of immobilized mismatch binding protein in mutation/SNP detection
  • Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check)
  • Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan)
  • The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection
  • pt. 1. Assays to detect and quantitate DNA repair activity
  • Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase
  • Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1)
  • Detection and quantitation of uracil DNA glycosylase activity
  • Detection of DNA deoxyribophosphodiesterase activity
  • O6-alkyguanine-DNA alkyltransferase assay
  • The E. coli vsr endonuclease: assaying activity in vitro and in vivo
  • Coupling of DNA helicase function to DNA strand exchange activity
Control code
47009799
Dimensions
unknown
Extent
1 online resource (x, 209 pages)
Form of item
online
Isbn
9780585354330
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)47009799
Label
DNA repair protocols : prokaryotic systems, edited by Pat Vaughan
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Detection and quanitation of recBCD enzymer (exonuclease V) activity
  • Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima
  • MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance
  • pt. 2. Use of DNA repair enzymes as tools in molecular biology
  • Flexible genetic engineering using RecA protein
  • Interspecies recombination and mismatch repair: generation of mosaic genes and genomes
  • The use of immobilized mismatch binding protein in mutation/SNP detection
  • Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check)
  • Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan)
  • The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection
  • pt. 1. Assays to detect and quantitate DNA repair activity
  • Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase
  • Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1)
  • Detection and quantitation of uracil DNA glycosylase activity
  • Detection of DNA deoxyribophosphodiesterase activity
  • O6-alkyguanine-DNA alkyltransferase assay
  • The E. coli vsr endonuclease: assaying activity in vitro and in vivo
  • Coupling of DNA helicase function to DNA strand exchange activity
Control code
47009799
Dimensions
unknown
Extent
1 online resource (x, 209 pages)
Form of item
online
Isbn
9780585354330
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)47009799

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