The Resource Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor, by John Thomas Gohring, (electronic resource)

Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor, by John Thomas Gohring, (electronic resource)

Label
Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor
Title
Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor
Statement of responsibility
by John Thomas Gohring
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
We have demonstrated label-free detection of CD4+ and CD8+ T-Lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. For T-Lymphocyte cell detection, white blood cells were isolated from healthy blood and the concentrations adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner capillary surface of the OFRR. Sensing results show excellent capture of CD4+ and CD8+ T-Lymphocytes at medically significant concentrations with a detection time of approximately 30 minutes. Protein biomarkers have recently been heavily researched in their roles in the detection, quantification, and monitoring of aggressive types of breast cancer. We describe a novel, label-free approach for detecting the HER2 extra-cellular domain breast cancer biomarker in human serum samples using the opto-fluidic ring resonator (OFRR). HER2 proteins were spiked in serum at varying concentrations. Results show that the OFRR is able to detect HER2 at medically relevant concentrations in serum ranging from 13 ng/mL to 100 ng/mL in 30 minutes. Our work will lead to a device that can be used as a tool for monitoring disease progression in a low cost sensing setup
Cataloging source
MUU
http://library.link/vocab/creatorDate
1984-
http://library.link/vocab/creatorName
Gohring, John Thomas
Degree
M.S.
Dissertation year
2009.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Fan, Xudong
http://library.link/vocab/subjectName
  • Optofluidics
  • Biosensors
  • T cells
  • CD4 antigen
  • HER-2 protein
  • Biochemical markers
  • Breast
Target audience
specialized
Label
Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor, by John Thomas Gohring, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Xudong Fan
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
558635057
Extent
1 online resource (vi, 47 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
MU: Access is limited to the campuses of the University of Missouri.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)558635057
Label
Detection of CD4 and CD8 t-lymphocytes and HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor, by John Thomas Gohring, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on March 10, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Dr. Xudong Fan
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
558635057
Extent
1 online resource (vi, 47 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
MU: Access is limited to the campuses of the University of Missouri.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)558635057

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
    • Engineering Library & Technology CommonsBorrow it
      W2001 Lafferre Hall, Columbia, MO, 65211, US
      38.946102 -92.330125
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