The Resource Development of a FRET biosensor to detect the pathogen mycoplasma capricolum, by Michelle Anne Windsor Kramer, (electronic resource)

Development of a FRET biosensor to detect the pathogen mycoplasma capricolum, by Michelle Anne Windsor Kramer, (electronic resource)

Label
Development of a FRET biosensor to detect the pathogen mycoplasma capricolum
Title
Development of a FRET biosensor to detect the pathogen mycoplasma capricolum
Statement of responsibility
by Michelle Anne Windsor Kramer
Title variation
Development of a Fluorescence Resonance Energy Transfer biosensor to detect the pathogen mycoplasma capricolum
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Mycoplasmas are a species of bacteria that invade any type of cell and are adaptable to changing environments. We are developing an optical biosensor based on Fluorescence Resonance Energy Transfer (FRET) for detection of these pathogens, specifically for M. capricolum. Three different anti-peptide antibodies, (E, F1, and F2), were conjugated with AF-546 dye (donor). Then the antibodies were complexed to Protein G labeled with AF-594 dye (acceptor). When these detection complexes were bound to bacteria or specific peptide, there was a change in the 3-D conformation of the antibody causing a measurable change in acceptor emission. [alpha]-pep F1 had the most distinct change at 12% so it was used in two different systems (organic fluorophores and a gold nanoparticle quenching system) and immobilized onto optical fibers. Our data illustrates that fiber optic biosensors measure conformational changes occurring when antibodies bind antigens and thus is a viable detection method for mycoplasmas
Cataloging source
MUU
http://library.link/vocab/creatorName
Windsor Kramer, Michelle Anne
Degree
M.S.
Dissertation year
2005.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Grant, Sheila Ann
http://library.link/vocab/subjectName
  • Biosensors
  • Fluorescent probes
  • Energy transfer
Target audience
specialized
Label
Development of a FRET biosensor to detect the pathogen mycoplasma capricolum, by Michelle Anne Windsor Kramer, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (January 11, 2006)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
77593158
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System details
Mode of access: World Wide Web
Label
Development of a FRET biosensor to detect the pathogen mycoplasma capricolum, by Michelle Anne Windsor Kramer, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (January 11, 2006)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
77593158
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System details
Mode of access: World Wide Web

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