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The Resource Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)
Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)
Resource Information
The item Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from all library branches.
Resource Information
The item Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from all library branches.
- Summary
- Helicobacter hepaticus causes hepatitis in mice. Infections with H. hepaticus can invalidate animal research studies and early detection helps curtail the spread of infection. The objective of this study was to develop a quick and accurate biosensor platform for the detection of H. hepaticus in fecal samples. This research investigated an optical immunosensor using capillary waveguides and a competitive immunoassay technique. H. hepaticus was immobilized to the inner wall of the capillary. A H. hepaticus antibody was conjugated to AlexaFluor 546 to serve as the fluorescent tracer and added to samples containing H. hepaticus. Sample H. hepaticus (analyte) bound to the antibodies in solution, thereby preventing the antibodies from attaching to the immobilized H. hepaticus. Several methods were utilized to analyze the fluorescence resulting from the immunoassay. Results showed the biosensor is capable of detecting low levels of H. hepaticus (1.0 ng) in an assay time of approximately one hour
- Language
- eng
- Note
-
- The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
- Title from title screen of research.pdf file viewed on (June 27, 2007)
- Label
- Development of a capillary based helicobacter hepaticus biosensor
- Title
- Development of a capillary based helicobacter hepaticus biosensor
- Statement of responsibility
- by Theodore Seth Thomas
- Language
- eng
- Summary
- Helicobacter hepaticus causes hepatitis in mice. Infections with H. hepaticus can invalidate animal research studies and early detection helps curtail the spread of infection. The objective of this study was to develop a quick and accurate biosensor platform for the detection of H. hepaticus in fecal samples. This research investigated an optical immunosensor using capillary waveguides and a competitive immunoassay technique. H. hepaticus was immobilized to the inner wall of the capillary. A H. hepaticus antibody was conjugated to AlexaFluor 546 to serve as the fluorescent tracer and added to samples containing H. hepaticus. Sample H. hepaticus (analyte) bound to the antibodies in solution, thereby preventing the antibodies from attaching to the immobilized H. hepaticus. Several methods were utilized to analyze the fluorescence resulting from the immunoassay. Results showed the biosensor is capable of detecting low levels of H. hepaticus (1.0 ng) in an assay time of approximately one hour
- Cataloging source
- MUU
- http://library.link/vocab/creatorName
- Thomas, Theodore Seth
- Dissertation year
- 2006.
- Granting institution
- Thesis (M.S.) University of Missouri-Columbia
- Illustrations
- illustrations
- Index
- no index present
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- theses
- http://library.link/vocab/relatedWorkOrContributorName
- Grant, Sheila Ann
- http://library.link/vocab/subjectName
-
- Helicobacter infections
- Biosensors
- Pathogenic microorganisms
- Target audience
- specialized
- Label
- Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)
- Note
-
- The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
- Title from title screen of research.pdf file viewed on (June 27, 2007)
- Bibliography note
- Includes bibliographical references
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- mixed
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Control code
- 145741373
- Dimensions
- unknown
- Form of item
- electronic
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Specific material designation
- remote
- System control number
- (OCoLC)145741373
- System details
- Mode of access: World Wide Web
- Label
- Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)
- Note
-
- The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
- Title from title screen of research.pdf file viewed on (June 27, 2007)
- Bibliography note
- Includes bibliographical references
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- mixed
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Control code
- 145741373
- Dimensions
- unknown
- Form of item
- electronic
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Specific material designation
- remote
- System control number
- (OCoLC)145741373
- System details
- Mode of access: World Wide Web
Library Locations
-
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Engineering Library & Technology CommonsBorrow itW2001 Lafferre Hall, Columbia, MO, 65211, US38.946102 -92.330125
-
Fisher Delta Research CenterBorrow it2-64 Agricultural Bldg, Columbia, MO, 65201, US38.958397 -92.303491
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Geological Sciences LibraryBorrow it201 Geological Sciences, Columbia, MO, 65211, US38.947375 -92.329062
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J. Otto Lottes Health Sciences LibraryBorrow it1 Hospital Dr, Columbia, MO, 65201, US38.939544 -92.328377
-
Journalism LibraryBorrow it102 Reynolds Jrnlism Institute, Columbia, MO, 65211, US38.947290 -92.328025
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Mathematical Sciences LibraryBorrow it104 Ellis Library, Columbia, MO, 65201, US38.944377 -92.326537
-
University ArchivesBorrow itColumbia, MO, 65201, US
-
University Archives McAlester AnnexBorrow it703 Lewis Hall, Columbia, MO, 65211, US38.934630 -92.342290
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University of Missouri Libraries DepositoryBorrow it2908 Lemone Blvd, Columbia, MO, 65211, US38.919360 -92.291620
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Zalk Veterinary Medical LibraryBorrow itVeterinary Medicine West, Columbia, MO, 65211, US38.941099 -92.317911
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Development-of-a-capillary-based-helicobacter/v9zpSkZZnFk/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Development-of-a-capillary-based-helicobacter/v9zpSkZZnFk/">Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Development-of-a-capillary-based-helicobacter/v9zpSkZZnFk/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Development-of-a-capillary-based-helicobacter/v9zpSkZZnFk/">Development of a capillary based helicobacter hepaticus biosensor, by Theodore Seth Thomas, (electronic resource)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
