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The Resource Development of a composite acellular tissue graft for musculoskeletal tissue engineering, by Sarah Elizabeth Smith

Development of a composite acellular tissue graft for musculoskeletal tissue engineering, by Sarah Elizabeth Smith

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Author
Contributor
Summary
A composite acellular tissue graft comprised of decellularized tendon conjugated with nanomaterials has been developed for musculoskeletal tissue engineering applications. The focus of this dissertation is on the development of composite grafts derived from decellularized human tendon conjugated with gold nanoparticles and hydroxyapatite nanoparticles for use in anterior cruciate ligament (ACL) reconstruction. Gold nanoparticles are used to promote remodeling, cellularity, and biological incorporation of grafts. Hydroxyapatite nanoparticles are used to promote osseointegration, cellularity, and to enhance the graft/bone interface. These composite grafts along with several other variations, were characterized in vitro using a variety of cell-based assays including cell viability, cell proliferation, and cell migration assays. Two in vivo studies were conducted. A green fluorescent protein (GFP) porcine model was investigated as a new method to evaluate host tissue integration into soft tissue grafts as well as the in vivo biocompatibility of subcutaneously implanted composite grafts. Results demonstrate biocompatibility and remodeling of composite grafts and the value of using the GFP model as a qualitative method for assessing host tissue integration. A rabbit ACL reconstruction model was used to investigate graft remodeling in addition to the overall viability of using composite grafts to serve as a functional ACL replacement. Results demonstrate successful replacement of ACLs using composite grafts with enhanced remodeling from the addition of nanoparticles. Overall, studies demonstrate the success and potential further application of using composite grafts for musculoskeletal tissue engineering applications. Future studies will include expanding development of variations of these composite materials to address additional clinical needs
Language
eng
Work
Publication
Extent
1 online resource (xix, 273 pages)
Note
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Sheila Grant
  • Includes vita
Instance
Label
Development of a composite acellular tissue graft for musculoskeletal tissue engineering
Title
Development of a composite acellular tissue graft for musculoskeletal tissue engineering
Statement of responsibility
by Sarah Elizabeth Smith
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
A composite acellular tissue graft comprised of decellularized tendon conjugated with nanomaterials has been developed for musculoskeletal tissue engineering applications. The focus of this dissertation is on the development of composite grafts derived from decellularized human tendon conjugated with gold nanoparticles and hydroxyapatite nanoparticles for use in anterior cruciate ligament (ACL) reconstruction. Gold nanoparticles are used to promote remodeling, cellularity, and biological incorporation of grafts. Hydroxyapatite nanoparticles are used to promote osseointegration, cellularity, and to enhance the graft/bone interface. These composite grafts along with several other variations, were characterized in vitro using a variety of cell-based assays including cell viability, cell proliferation, and cell migration assays. Two in vivo studies were conducted. A green fluorescent protein (GFP) porcine model was investigated as a new method to evaluate host tissue integration into soft tissue grafts as well as the in vivo biocompatibility of subcutaneously implanted composite grafts. Results demonstrate biocompatibility and remodeling of composite grafts and the value of using the GFP model as a qualitative method for assessing host tissue integration. A rabbit ACL reconstruction model was used to investigate graft remodeling in addition to the overall viability of using composite grafts to serve as a functional ACL replacement. Results demonstrate successful replacement of ACLs using composite grafts with enhanced remodeling from the addition of nanoparticles. Overall, studies demonstrate the success and potential further application of using composite grafts for musculoskeletal tissue engineering applications. Future studies will include expanding development of variations of these composite materials to address additional clinical needs
Cataloging source
MUU
http://library.link/vocab/creatorName
Smith, Sarah Elizabeth
Degree
Ph. D.
Dissertation note
Thesis
Dissertation year
2015.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Grant, Sheila Ann
http://library.link/vocab/subjectName
  • Transplantation of organs, tissues, etc.
  • Anterior cruciate ligament
  • Nanoparticles
  • Tissue engineering
  • Musculoskeletal system
Label
Development of a composite acellular tissue graft for musculoskeletal tissue engineering, by Sarah Elizabeth Smith
Instantiates
Publication
Note
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Sheila Grant
  • Includes vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
975487152
Extent
1 online resource (xix, 273 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)975487152
Label
Development of a composite acellular tissue graft for musculoskeletal tissue engineering, by Sarah Elizabeth Smith
Publication
Note
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Sheila Grant
  • Includes vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
975487152
Extent
1 online resource (xix, 273 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)975487152

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