The Resource Development of a flexible image-based approach for studying signal transduction in isolated arterioles, by Srikanth R. Ella, (electronic resource)

Development of a flexible image-based approach for studying signal transduction in isolated arterioles, by Srikanth R. Ella, (electronic resource)

Label
Development of a flexible image-based approach for studying signal transduction in isolated arterioles
Title
Development of a flexible image-based approach for studying signal transduction in isolated arterioles
Statement of responsibility
by Srikanth R. Ella
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Myogenic constriction in arterioles occurs when membrane tension leads to smooth muscle depolarization and voltage-gated calcium channel activation. A barrier to understanding the exact signaling events is the limited ability of glass electrodes to follow temporal changes in membrane potential (Em). This study aimed to develop an imaging station to measure Em, [Ca2+]i and diameter in isolated arterioles. A Forster Resonance Enery Transfer (FRET) approach was proposed for Em meaurement due to enhanced sensitivity and temporal responsiveness. FRET indicators used were CC2-DMPE (Coumarin-labelled Phospholipid, Donor, ex: 405nm) and DisBAC4(3) (Oxonol, acceptor). For measurement of [Ca2+]i Fluo-4 AM (ex 488nm) was used. Initial studies examined toxicity of the multiple fluorophores. Isolated cells remained viable after loading with the fluorophores. Arterioles maintained myogenic, and agonist, responsiveness pre and post dye loading suggesting a lack of toxicity. Spectral characteristics of the fluorophores used in the project required specific optical filter units and development of a. custom built optical system. Using the system, FRET was demonstrated, as was the ability to detect changes in fluorescence signal to known depolarizing stimuli. The developed system is now ready for translation to the study of myogenic signaling in intact and functional arterioles
Cataloging source
MUU
http://library.link/vocab/creatorName
Ella, Srikanth R
Degree
M.S.
Dissertation year
2007.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Hill, Michael
http://library.link/vocab/subjectName
  • Cellular signal transduction
  • Blood-vessels
Target audience
specialized
Label
Development of a flexible image-based approach for studying signal transduction in isolated arterioles, by Srikanth R. Ella, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on May 11, 2009)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
320779747
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)320779747
System details
Mode of access: World Wide Web
Label
Development of a flexible image-based approach for studying signal transduction in isolated arterioles, by Srikanth R. Ella, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on May 11, 2009)
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
320779747
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)320779747
System details
Mode of access: World Wide Web

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