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The Resource Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties, by Nicole Poythress Waters, (electronic resource)

Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties, by Nicole Poythress Waters, (electronic resource)

Creator
Author
Contributor
Summary
Post-traumatic osteoarthritis (PTOA) is a very painful and debilitating disease that is associated with mechanical injury to articular cartilage, yet the severity of trauma required to induce it is unknown. The objective of this thesis work was to develop a clinically-relevant ex vivo PTOA model with repeatable single impact having constant velocity and maximum strain (i.e. severity categories normalized to thickness of cartilage explant) to study their effect on cartilage PTOA biomarkers: cell viability, extracellular matrix, and material properties. A 25 kN servo-hydraulic test machine was used to measure thickness and subsequently deliver an impact load at velocity (V) of 1 or 100 mm/sec to a maximum strain (S) of 10, 30, or 50% (six velocity: strain test groups) to cartilage explants. Non-impact (0V:0S) and sham controls were included. Thereafter, explants were cultured in supplemented media for twelve days. Cell viability was analyzed post-injury at day 0 and 12 as was cartilage extracellular matrix for collagen (specifically hydroxyproline) and glycosaminoglycan (GAG) content. Media was changed after day 1, 2, 3, 6, 9, and 12 and tested for GAG, collagen II synthesis, nitric oxide, and prostaglandin E2 (PGE2). Material testing measuring the elastic, equilibrium, and dynamic moduli of cartilage explants was performed at day 0 (pre-injury) and days 6 and 12. Cell death, GAG and PGE2 release to media (at day 1) were strain dependent with 50% strain groups being significantly different than others, and high velocity prolonging release of PGE2 up to day 6. These results indicate key biomarkers involved in early PTOA
Language
eng
Work
Publication
Extent
1 online resource (xii, 101 pages)
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 19, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Drs. Sheila Grant and James Cook
Instance
Label
Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties
Title
Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties
Statement of responsibility
by Nicole Poythress Waters
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
Post-traumatic osteoarthritis (PTOA) is a very painful and debilitating disease that is associated with mechanical injury to articular cartilage, yet the severity of trauma required to induce it is unknown. The objective of this thesis work was to develop a clinically-relevant ex vivo PTOA model with repeatable single impact having constant velocity and maximum strain (i.e. severity categories normalized to thickness of cartilage explant) to study their effect on cartilage PTOA biomarkers: cell viability, extracellular matrix, and material properties. A 25 kN servo-hydraulic test machine was used to measure thickness and subsequently deliver an impact load at velocity (V) of 1 or 100 mm/sec to a maximum strain (S) of 10, 30, or 50% (six velocity: strain test groups) to cartilage explants. Non-impact (0V:0S) and sham controls were included. Thereafter, explants were cultured in supplemented media for twelve days. Cell viability was analyzed post-injury at day 0 and 12 as was cartilage extracellular matrix for collagen (specifically hydroxyproline) and glycosaminoglycan (GAG) content. Media was changed after day 1, 2, 3, 6, 9, and 12 and tested for GAG, collagen II synthesis, nitric oxide, and prostaglandin E2 (PGE2). Material testing measuring the elastic, equilibrium, and dynamic moduli of cartilage explants was performed at day 0 (pre-injury) and days 6 and 12. Cell death, GAG and PGE2 release to media (at day 1) were strain dependent with 50% strain groups being significantly different than others, and high velocity prolonging release of PGE2 up to day 6. These results indicate key biomarkers involved in early PTOA
Cataloging source
MUU
http://library.link/vocab/creatorDate
1984-
http://library.link/vocab/creatorName
Waters, Nicole Poythress
Degree
M.S.
Dissertation year
2009.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1965-
http://library.link/vocab/relatedWorkOrContributorName
  • Cook, James L.
  • Grant, Sheila Ann
http://library.link/vocab/subjectName
  • Osteoarthritis
  • Articular cartilage
  • Knee
  • Joints
  • Joints
Target audience
specialized
Label
Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties, by Nicole Poythress Waters, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 19, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Drs. Sheila Grant and James Cook
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
540741483
Extent
1 online resource (xii, 101 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)540741483
Label
Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties, by Nicole Poythress Waters, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 19, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Thesis advisor: Drs. Sheila Grant and James Cook
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
540741483
Extent
1 online resource (xii, 101 pages)
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)540741483

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