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The Resource Directed enzyme evolution : screening and selection methods, edited by Frances H. Arnold and George Georgiou

Directed enzyme evolution : screening and selection methods, edited by Frances H. Arnold and George Georgiou

Label
Directed enzyme evolution : screening and selection methods
Title
Directed enzyme evolution
Title remainder
screening and selection methods
Statement of responsibility
edited by Frances H. Arnold and George Georgiou
Contributor
Subject
Genre
Language
eng
Member of
Cataloging source
NLM
Illustrations
  • illustrations
  • plates
Index
index present
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 230
Nature of contents
bibliography
NLM call number
  • W1
  • QU 135
NLM item number
  • ME9616J v.230 2003
  • D598 2003
http://library.link/vocab/relatedWorkOrContributorName
  • Arnold, Frances Hamilton
  • Georgiou, George
Series statement
Methods in molecular biology
Series volume
230
http://library.link/vocab/subjectName
  • Molecular evolution
  • Gene libraries
  • Protein engineering
  • Genetic engineering
  • Molecular evolution
  • Directed Molecular Evolution
  • Enzymes
  • Evolution, Molecular
  • Gene Library
  • Protein Engineering
Label
Directed enzyme evolution : screening and selection methods, edited by Frances H. Arnold and George Georgiou
Instantiates
Publication
Bibliography note
Includes bibliographic references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Pt. 1. Genetic selections -- Genetic complementation protocols / Jessica L. Sneeden -- Use of Pol 1-Deficient E. coli for functional complementation of DNA polymerase / Manel Camps -- Selection of novel eukaryotic DNA polymerases by mutagenesis and genetic complementation of yeast / Ranga N. Venkatesan -- Autogene selections / jijumon Chelliserrykattil -- Selection for soluble proteins via fusion with chloramphenicol acetyltransferase / Volker Sieber -- Proside: a phage-based method for selecting thermostable proteins / Andreas Martin -- Minimization of proteins by random fragmentation and selection / Gary W. Rudgers -- pt. 2. Screens for enzymes -- Evaluating a screen and analysis of mutant libraries / Oriana Salazar -- Screening mutant libraries in Saccharomyces cerevisiae / Thomas Bulter -- Solid-phase screening using digital image analysis / Alexander V. Tobias -- Screening for thermostability / Patrick C. Cirino -- High-throughput screening of mutant a-amylase libraries for increased activity at 129C / Holger Berk -- High-throughput carbon monoxide binding assay for cytochromes P450 / Christopher R. Otey -- High-throughput screen for aromatic hydroxylation / Christopher R. Otey -- Colorimetric screen for aliphatic hydroxylation by cytochrome P450 using p-ntirophenyl-substituted alkanes / Edgardo T. Farinas -- High-throughput screens based on NAD(P)H depletion / Anton Glieder -- High-throughput tetramethylbenzidine (TMB) screen for peroxidases / Radu Georgescu -- Screen for oxidases by detection of hydrogen peroxide with horseradish peroxidase / Lianhong Sun -- Colorimetric dehydrogenase screen based on NAD(P)H generation / Kimberly M. Mayer -- Colorimetric assays for screening laccases / Miguel Alcalde -- pH sensing agar plate assays for esterolytic enzyme activity / Karl E. Griswold -- A pH-indicator-based screen for hydrolytic haloalkane dehalogenase / Huimin Zhao -- Detection of aromatic a-hydroxyketones with tetrazolium salts / Michael Breuer -- Selection of heat-stable Clostridium cellulovorans cellulases after in vivo recombination / Koichiro Murashima -- Screening and selection strategies for disulfide isomerase activity /Ronald Lafond -- An overview of high-throughput screening systems for enantioselective enyzmatic transformations / Manfred T. Reetz -- Select protocols of high-throughput ee-screening systems for assaying enantioselective enzymes / Manfred T. Reetz -- Directed evolution of the substrate specifications of a site-specific recombinase and an aminoacyl-tRNA synthetase using fluorescence-activated cell sorting (FACS) / Stephen W. Santoro -- Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities /Christian Heinis -- High-throughput FACS method for directed evolution of substrate specificity / Mark J. Olsen -- Improving protein folding efficiency by directed evolution using GFP folding reporter / Geoffrey S. Waldo
Control code
52400248
Dimensions
24 cm
Extent
xv, 383 pages, 4 unnumbered pages of plates
Isbn
9781588292865
Isbn Type
(alk. paper)
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)
Label
Directed enzyme evolution : screening and selection methods, edited by Frances H. Arnold and George Georgiou
Publication
Bibliography note
Includes bibliographic references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Pt. 1. Genetic selections -- Genetic complementation protocols / Jessica L. Sneeden -- Use of Pol 1-Deficient E. coli for functional complementation of DNA polymerase / Manel Camps -- Selection of novel eukaryotic DNA polymerases by mutagenesis and genetic complementation of yeast / Ranga N. Venkatesan -- Autogene selections / jijumon Chelliserrykattil -- Selection for soluble proteins via fusion with chloramphenicol acetyltransferase / Volker Sieber -- Proside: a phage-based method for selecting thermostable proteins / Andreas Martin -- Minimization of proteins by random fragmentation and selection / Gary W. Rudgers -- pt. 2. Screens for enzymes -- Evaluating a screen and analysis of mutant libraries / Oriana Salazar -- Screening mutant libraries in Saccharomyces cerevisiae / Thomas Bulter -- Solid-phase screening using digital image analysis / Alexander V. Tobias -- Screening for thermostability / Patrick C. Cirino -- High-throughput screening of mutant a-amylase libraries for increased activity at 129C / Holger Berk -- High-throughput carbon monoxide binding assay for cytochromes P450 / Christopher R. Otey -- High-throughput screen for aromatic hydroxylation / Christopher R. Otey -- Colorimetric screen for aliphatic hydroxylation by cytochrome P450 using p-ntirophenyl-substituted alkanes / Edgardo T. Farinas -- High-throughput screens based on NAD(P)H depletion / Anton Glieder -- High-throughput tetramethylbenzidine (TMB) screen for peroxidases / Radu Georgescu -- Screen for oxidases by detection of hydrogen peroxide with horseradish peroxidase / Lianhong Sun -- Colorimetric dehydrogenase screen based on NAD(P)H generation / Kimberly M. Mayer -- Colorimetric assays for screening laccases / Miguel Alcalde -- pH sensing agar plate assays for esterolytic enzyme activity / Karl E. Griswold -- A pH-indicator-based screen for hydrolytic haloalkane dehalogenase / Huimin Zhao -- Detection of aromatic a-hydroxyketones with tetrazolium salts / Michael Breuer -- Selection of heat-stable Clostridium cellulovorans cellulases after in vivo recombination / Koichiro Murashima -- Screening and selection strategies for disulfide isomerase activity /Ronald Lafond -- An overview of high-throughput screening systems for enantioselective enyzmatic transformations / Manfred T. Reetz -- Select protocols of high-throughput ee-screening systems for assaying enantioselective enzymes / Manfred T. Reetz -- Directed evolution of the substrate specifications of a site-specific recombinase and an aminoacyl-tRNA synthetase using fluorescence-activated cell sorting (FACS) / Stephen W. Santoro -- Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities /Christian Heinis -- High-throughput FACS method for directed evolution of substrate specificity / Mark J. Olsen -- Improving protein folding efficiency by directed evolution using GFP folding reporter / Geoffrey S. Waldo
Control code
52400248
Dimensions
24 cm
Extent
xv, 383 pages, 4 unnumbered pages of plates
Isbn
9781588292865
Isbn Type
(alk. paper)
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)

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