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The Resource Electrospinning collagen and hyaluronic acid nanofiber meshes

Electrospinning collagen and hyaluronic acid nanofiber meshes

Creator
Author
Summary
Osteoporosis is a worldwide burden affecting over 200 million people. There are many risk factors associated with osteoporosis, but they all lead to decreased bone strength and an increased chance of bone fracture. The field of tissue engineering could be used to create bone substitute materials that can increase bone strength or promote new bone growth. Collagen and hyaluronic acid (HA) are particularly interesting due to their roles in the extracellular matrix (ECM) of humans and their ability to stimulate bone forming cells. These materials can be processed into nanofiber scaffolds using a technique called electrospinning; a process that applies high voltage to a polymer solution to draw out nanofibers that are collected on a ground plate. An electrospinning solution of collagen/HA was synthesized, and the electrospinning apparatus was adjusted to generate uniform nanofiber meshes with consistent nanofiber diameter. The meshes were then crosslinked to render them insoluble in aqueous solutions, and gold nanoparticles (AuNPs) were conjugated to the surface to promote cellular in-growth. Scanning electron microscope (SEM) was utilized to image the nanofiber mesh surface and electron dispersive spectroscopy (EDS) was used to quantify the charged particles on the mesh as gold. Fourier transform infrared spectroscopy (FT-IR) was used to find the absorption spectrum and bonding changes after each step. A WST-1 assay was also performed to determine the potential biocompatibility. The results show that it was possible to develop scaffolds that were insoluble in aqueous solutions and promoted cellular attachment and proliferation. In conclusion, nanofiber meshes made from collagen/HA could be utilized as a tissue engineered scaffold to promote bone growth
Language
eng
Work
Publication
Extent
1 online resource (viii, 82 pages)
Note
Advisor: Sheila Ann Grant
Instance
Label
Electrospinning collagen and hyaluronic acid nanofiber meshes
Title
Electrospinning collagen and hyaluronic acid nanofiber meshes
Creator
Author
Subject
Genre
Language
eng
Summary
Osteoporosis is a worldwide burden affecting over 200 million people. There are many risk factors associated with osteoporosis, but they all lead to decreased bone strength and an increased chance of bone fracture. The field of tissue engineering could be used to create bone substitute materials that can increase bone strength or promote new bone growth. Collagen and hyaluronic acid (HA) are particularly interesting due to their roles in the extracellular matrix (ECM) of humans and their ability to stimulate bone forming cells. These materials can be processed into nanofiber scaffolds using a technique called electrospinning; a process that applies high voltage to a polymer solution to draw out nanofibers that are collected on a ground plate. An electrospinning solution of collagen/HA was synthesized, and the electrospinning apparatus was adjusted to generate uniform nanofiber meshes with consistent nanofiber diameter. The meshes were then crosslinked to render them insoluble in aqueous solutions, and gold nanoparticles (AuNPs) were conjugated to the surface to promote cellular in-growth. Scanning electron microscope (SEM) was utilized to image the nanofiber mesh surface and electron dispersive spectroscopy (EDS) was used to quantify the charged particles on the mesh as gold. Fourier transform infrared spectroscopy (FT-IR) was used to find the absorption spectrum and bonding changes after each step. A WST-1 assay was also performed to determine the potential biocompatibility. The results show that it was possible to develop scaffolds that were insoluble in aqueous solutions and promoted cellular attachment and proliferation. In conclusion, nanofiber meshes made from collagen/HA could be utilized as a tissue engineered scaffold to promote bone growth
Cataloging source
MUU
http://library.link/vocab/creatorName
Fischer, Rachael
Degree
M.S.
Dissertation note
Thesis
Dissertation year
2011.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia,
Index
no index present
Literary form
non fiction
Nature of contents
dictionaries
Label
Electrospinning collagen and hyaluronic acid nanofiber meshes
Instantiates
Publication
Contributor
Thesis advisor
Note
Advisor: Sheila Ann Grant
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
876749964
Extent
1 online resource (viii, 82 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Specific material designation
remote
System control number
(OCoLC)876749964
Label
Electrospinning collagen and hyaluronic acid nanofiber meshes
Publication
Contributor
Thesis advisor
Note
Advisor: Sheila Ann Grant
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
876749964
Extent
1 online resource (viii, 82 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Specific material designation
remote
System control number
(OCoLC)876749964

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