Coverart for item
The Resource Enzymes in synthetic biology, edited by Nigel Scrutton

Enzymes in synthetic biology, edited by Nigel Scrutton

Label
Enzymes in synthetic biology
Title
Enzymes in synthetic biology
Statement of responsibility
edited by Nigel Scrutton
Contributor
Editor
Subject
Language
eng
Summary
Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. Detailed methods for laboratory use of enzymes in synthetic biology applicationsInformative case history examples illustrating how enzyme and metabolic engineering are used to generate new productsEmphasises latest developments in laboratory automation for the engineering of biologyCovers many aspects of the design, build, test, learn cycle used in synthetic biology
Member of
Is part of
Cataloging source
N$T
Dewey number
660.6
Illustrations
illustrations
Index
index present
Language note
Text in English
LC call number
  • TA164
  • QP601
LC item number
  • .E59 2018
  • .M49 v.608
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Scrutton, Nigel S.
Series statement
Methods in enzymology
Series volume
volume six hundred and eight
http://library.link/vocab/subjectName
  • Enzymes
  • Synthetic biology
  • Enzymes
  • Synthetic Biology
  • SCIENCE
  • TECHNOLOGY & ENGINEERING
  • Enzymes
  • Synthetic biology
Label
Enzymes in synthetic biology, edited by Nigel Scrutton
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Enzyme Discovery; Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design; Abstract; 1 Introduction; 2 Enzyme Selection; 3 Pathway Design; 4 Summary and Conclusion; Acknowledgments; Section II: Enzyme Engineering Tools and Platforms; Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping; Abstract; 1 Introduction; 2 The Cell-Free Metabolic Engineering Framework; 3 S12 Lysate Preparation for Cell-Free Metabolic Engineering; 4 Mix-and-Match Cell-Free Metabolic Engineering
  • 5 Cell-Free Protein Synthesis-Driven Metabolic Engineering6 Summary and Conclusions; Acknowledgments; Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution; Abstract; 1 Introduction; 2 Methods; 3 Library Ligation, Transformation, and Quality Control; 4 Summary and Conclusion; Acknowledgments; Section III: Enzyme Families: Parts and Platforms for Chemical Diversity; Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether; Abstract; 1 Introduction
  • 2 Sesquiterpenes Production and Purification3 Enzymatic Synthesis of Cyclic Ether; 4 Conclusion; Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity; Abstract; 1 Introduction; 2 In Vitro Reconstitution of the MVA Pathway and Targeted Engineering of Value-Added Terpenoids; 3 Genome Mining of Terpene Cyclases and the Generation of Chemical Diversity; 4 Conclusion; Acknowledgments; Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications
  • Abstract1 Introduction; 2 Protein Engineering; 3 Biotransformations, Intensification, and Scale-Up; 4 In Vitro and in vivo Cascades; 5 Conclusions; Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins; Abstract; 1 Introduction; 2 Single Mutants Generation; 3 Combining Mutations; 4 Summary and Conclusions; Acknowledgments; Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology; Abstract; 1 Introduction; 2 Expression and Purification of Microbial Cytochrome P450 Enzymes
  • 3 Expression and Purification of Eukaryotic P450s in E. coli4 Expression and Characterization of P450 Redox Partner Systems; 5 Determination of P450 Purity, Concentration, and Other Properties Using UV-Visible Spectroscopy; 6 Using Cytochromes P450 for Oxidative Transformations; 7 Crystallization and Structural Analysis of P450S; 8 Applications of P450 Enzymes in Synthetic Biology; 9 Summary and Conclusion; Section IV: Genome Engineering for Enzyme Pathways
Control code
1050437344
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (xviii, 439 pages)
File format
unknown
Form of item
online
Isbn
9780128151495
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
http://library.link/vocab/ext/overdrive/overdriveId
9780128151495
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1050437344
Label
Enzymes in synthetic biology, edited by Nigel Scrutton
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Enzyme Discovery; Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design; Abstract; 1 Introduction; 2 Enzyme Selection; 3 Pathway Design; 4 Summary and Conclusion; Acknowledgments; Section II: Enzyme Engineering Tools and Platforms; Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping; Abstract; 1 Introduction; 2 The Cell-Free Metabolic Engineering Framework; 3 S12 Lysate Preparation for Cell-Free Metabolic Engineering; 4 Mix-and-Match Cell-Free Metabolic Engineering
  • 5 Cell-Free Protein Synthesis-Driven Metabolic Engineering6 Summary and Conclusions; Acknowledgments; Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution; Abstract; 1 Introduction; 2 Methods; 3 Library Ligation, Transformation, and Quality Control; 4 Summary and Conclusion; Acknowledgments; Section III: Enzyme Families: Parts and Platforms for Chemical Diversity; Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether; Abstract; 1 Introduction
  • 2 Sesquiterpenes Production and Purification3 Enzymatic Synthesis of Cyclic Ether; 4 Conclusion; Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity; Abstract; 1 Introduction; 2 In Vitro Reconstitution of the MVA Pathway and Targeted Engineering of Value-Added Terpenoids; 3 Genome Mining of Terpene Cyclases and the Generation of Chemical Diversity; 4 Conclusion; Acknowledgments; Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications
  • Abstract1 Introduction; 2 Protein Engineering; 3 Biotransformations, Intensification, and Scale-Up; 4 In Vitro and in vivo Cascades; 5 Conclusions; Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins; Abstract; 1 Introduction; 2 Single Mutants Generation; 3 Combining Mutations; 4 Summary and Conclusions; Acknowledgments; Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology; Abstract; 1 Introduction; 2 Expression and Purification of Microbial Cytochrome P450 Enzymes
  • 3 Expression and Purification of Eukaryotic P450s in E. coli4 Expression and Characterization of P450 Redox Partner Systems; 5 Determination of P450 Purity, Concentration, and Other Properties Using UV-Visible Spectroscopy; 6 Using Cytochromes P450 for Oxidative Transformations; 7 Crystallization and Structural Analysis of P450S; 8 Applications of P450 Enzymes in Synthetic Biology; 9 Summary and Conclusion; Section IV: Genome Engineering for Enzyme Pathways
Control code
1050437344
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (xviii, 439 pages)
File format
unknown
Form of item
online
Isbn
9780128151495
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
http://library.link/vocab/ext/overdrive/overdriveId
9780128151495
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1050437344

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...