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The Resource Exocytosis and endocytosis, edited by Andrei I. Ivanov

Exocytosis and endocytosis, edited by Andrei I. Ivanov

Label
Exocytosis and endocytosis
Title
Exocytosis and endocytosis
Statement of responsibility
edited by Andrei I. Ivanov
Contributor
Copyright holder
Editor
Subject
Genre
Language
eng
Summary
Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane, and organelle trafficking in cultured cells, and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms
Member of
Cataloging source
NLM
Dewey number
571.6
Illustrations
illustrations
Index
index present
LC call number
QH634.2
LC item number
E96 2014
Literary form
non fiction
Nature of contents
bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.1174 2014
http://library.link/vocab/relatedWorkOrContributorName
  • Ivanov, Andrei I.
  • Springer Science+Business Media
Series statement
  • Methods in molecular biology,
  • Springer protocols
Series volume
1174
http://library.link/vocab/subjectName
  • Exocytosis
  • Endocytosis
  • Exocytosis
  • Endocytosis
Label
Exocytosis and endocytosis, edited by Andrei I. Ivanov
Instantiates
Publication
Copyright
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1. Pharmacological inhibitors of exocytosis and endocytosis : novel bullets for old targets / Andrei I. Ivanov -- 2. Systematic analysis of endocytosis by cellular perturbations / Lena Kühling and Mario Schelhaas -- 3. Real-time detection of SNARE complex assembly with FRET using the tetracysteine system / Oleg Varlamov -- 4. Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides / Guoqiang Xu, Alessia Deglincerti, Jeremy S. Paige and Samie R. Jaffrey -- 5. Secretion of circular proteins using sortase / Karin Strijbis and Hidde L. Ploegh -- 6. Fractionation of subcellular membrane vesicles of epithelial and non-epithelial cells by OptiPrep[tm] density gradient ultracentrifugation / Xuhang Li and Mark Donowitz -- 7. Combining pulsed SILAC labeling and click-chemistry for quantitative secretome analysis / Katrin Eichelbaum and Jeroen Krijgsveld -- 8. Probabilistic density maps to study the spatial organization of endocytosis / Jean-Philippe Grossier, Bruno Goud, and Kristine Schauer -- 9. Use of Kaede and Kikume green-red fusions for live cell imaging of G protein-coupled receptors / Antje Schmidt, Burkhard Wiesner, Ralf Schülein and Anke Teichmann -- 10. HaloTag as a tool to investigate peroxisome dynamics in cultured mammalian cells / Marc Fransen -- 11. SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells / Emily H. Stoops, Glen A. Farr, Michael Hull and Michael J. Caplan -- 12. FlAsh-PALM : Super-resolution pointillist imaging with FlAsh-tetracysteine labeling / Mickaël Lelek, Francesca Di Nunzio, and Christophe Zimmer -- 13. Analysis of protein dynamics with tandem fluorescent protein timers / Anton Khmelinskii and Michael Knop -- 14. Synchronization of secretory cargos trafficking in populations of cells / Gaelle Boncompain and Franck Perez -- 15. Use of transmembrane FRET to investigate the internalization of glycosylated proteins / Yoshimi Haga and Tadashi Suzuki -- 16. A method to rapidly induce organelle-specific molecular activities and membrane tethering / Toru Komatsu and Takanari Inoue -- 17. A novel pair of split Venus fragments to detect protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays / Kazumasa Ohashi and Kensaku Mizuno -- 18. Real-time investigation of plasma membrane deformation and fusion pore expansion using polarized total internal reflection fluorescence microscopy / Daniel R. Passmore, Tejeshwar Rao, and Arun Anantharam -- 19. Nanocones to study initial steps of endocytosis / Sangmoo Jeong and Milos Galic -- 20. A novel permeabilization protocol to obtain intracellular 3D immunolabeling for electron tomography / Nuria Jiménez and Jan A. Post -- 21. VIS2FIX : rapid chemical fixation of vitreous sections for immuno-electron microscopy / Matthia A. Karreman and Elly G. Van Donselaar -- 22. Chemical genomics : characterizing target pathways for bioactive compounds using the endomembrane trafficking network / Cecilia Rodriguez-Furlán, Glenn R. Hicks, and Lorena Norambuena -- 23. Application of RNAi technology and fluorescent protein markers to study membrane traffic in C. elegans / Jachen A. Solinger, Dmitry Poteryaev, and Anne Spang -- 24. Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers / Anupma Jha and Linton M. Traub -- 25. A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling / Jeffrey S. Dason and Milton P. Charlton -- 26. Microfluidic devices for imaging trafficking events in vivo using genetic model organisms / Sudip Mondal and Sandhya P. Koushika -- 27. The "in situ" proximity ligation assay to probe protein-protein interactions in intact tissues / Arianna Bellucci, Chiara Firorentini, Michela Zaltieri, Cristina Missale and PierFranco Spano -- 28. Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy / Oleg Milberg, Muhibullah Tora, Akiko Shitara, Taishin Takuma, Andrius Masedunskas and Roberto Weigert -- 29. Measurement of dynamic F-actin changes during exocytosis / Peter Thorn
Control code
886574299
Dimensions
26 cm
Edition
Second edition.
Extent
xiii, 435 pages
Isbn
9781493909438
Isbn Type
(alk. paper)
Lccn
2014940962
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)
System control number
(OCoLC)886574299
Label
Exocytosis and endocytosis, edited by Andrei I. Ivanov
Publication
Copyright
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1. Pharmacological inhibitors of exocytosis and endocytosis : novel bullets for old targets / Andrei I. Ivanov -- 2. Systematic analysis of endocytosis by cellular perturbations / Lena Kühling and Mario Schelhaas -- 3. Real-time detection of SNARE complex assembly with FRET using the tetracysteine system / Oleg Varlamov -- 4. Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides / Guoqiang Xu, Alessia Deglincerti, Jeremy S. Paige and Samie R. Jaffrey -- 5. Secretion of circular proteins using sortase / Karin Strijbis and Hidde L. Ploegh -- 6. Fractionation of subcellular membrane vesicles of epithelial and non-epithelial cells by OptiPrep[tm] density gradient ultracentrifugation / Xuhang Li and Mark Donowitz -- 7. Combining pulsed SILAC labeling and click-chemistry for quantitative secretome analysis / Katrin Eichelbaum and Jeroen Krijgsveld -- 8. Probabilistic density maps to study the spatial organization of endocytosis / Jean-Philippe Grossier, Bruno Goud, and Kristine Schauer -- 9. Use of Kaede and Kikume green-red fusions for live cell imaging of G protein-coupled receptors / Antje Schmidt, Burkhard Wiesner, Ralf Schülein and Anke Teichmann -- 10. HaloTag as a tool to investigate peroxisome dynamics in cultured mammalian cells / Marc Fransen -- 11. SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells / Emily H. Stoops, Glen A. Farr, Michael Hull and Michael J. Caplan -- 12. FlAsh-PALM : Super-resolution pointillist imaging with FlAsh-tetracysteine labeling / Mickaël Lelek, Francesca Di Nunzio, and Christophe Zimmer -- 13. Analysis of protein dynamics with tandem fluorescent protein timers / Anton Khmelinskii and Michael Knop -- 14. Synchronization of secretory cargos trafficking in populations of cells / Gaelle Boncompain and Franck Perez -- 15. Use of transmembrane FRET to investigate the internalization of glycosylated proteins / Yoshimi Haga and Tadashi Suzuki -- 16. A method to rapidly induce organelle-specific molecular activities and membrane tethering / Toru Komatsu and Takanari Inoue -- 17. A novel pair of split Venus fragments to detect protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays / Kazumasa Ohashi and Kensaku Mizuno -- 18. Real-time investigation of plasma membrane deformation and fusion pore expansion using polarized total internal reflection fluorescence microscopy / Daniel R. Passmore, Tejeshwar Rao, and Arun Anantharam -- 19. Nanocones to study initial steps of endocytosis / Sangmoo Jeong and Milos Galic -- 20. A novel permeabilization protocol to obtain intracellular 3D immunolabeling for electron tomography / Nuria Jiménez and Jan A. Post -- 21. VIS2FIX : rapid chemical fixation of vitreous sections for immuno-electron microscopy / Matthia A. Karreman and Elly G. Van Donselaar -- 22. Chemical genomics : characterizing target pathways for bioactive compounds using the endomembrane trafficking network / Cecilia Rodriguez-Furlán, Glenn R. Hicks, and Lorena Norambuena -- 23. Application of RNAi technology and fluorescent protein markers to study membrane traffic in C. elegans / Jachen A. Solinger, Dmitry Poteryaev, and Anne Spang -- 24. Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers / Anupma Jha and Linton M. Traub -- 25. A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling / Jeffrey S. Dason and Milton P. Charlton -- 26. Microfluidic devices for imaging trafficking events in vivo using genetic model organisms / Sudip Mondal and Sandhya P. Koushika -- 27. The "in situ" proximity ligation assay to probe protein-protein interactions in intact tissues / Arianna Bellucci, Chiara Firorentini, Michela Zaltieri, Cristina Missale and PierFranco Spano -- 28. Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy / Oleg Milberg, Muhibullah Tora, Akiko Shitara, Taishin Takuma, Andrius Masedunskas and Roberto Weigert -- 29. Measurement of dynamic F-actin changes during exocytosis / Peter Thorn
Control code
886574299
Dimensions
26 cm
Edition
Second edition.
Extent
xiii, 435 pages
Isbn
9781493909438
Isbn Type
(alk. paper)
Lccn
2014940962
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations (some color)
System control number
(OCoLC)886574299

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