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The Resource Expressed sequence tags (ESTs : generation and analysis, edited by John Parkinson

Expressed sequence tags (ESTs : generation and analysis, edited by John Parkinson

Label
Expressed sequence tags (ESTs : generation and analysis
Title
Expressed sequence tags (ESTs
Title remainder
generation and analysis
Statement of responsibility
edited by John Parkinson
Contributor
Subject
Language
eng
Summary
Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In Expressed Sequence Tags (ESTs): Generation and Analysis, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Expressed Sequence Tags (ESTs): Generation and Analysis serves as an ideal reference for scientists continuing the vital investigation of genes and the genome
Member of
Cataloging source
COO
Dewey number
611/.0181663
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP620
LC item number
.E95 2009
Literary form
non fiction
NAL call number
QP625.N89
NAL item number
E97 2009
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 470
NLM item number
  • ME9616J v.533 2009
  • E96 2009
http://library.link/vocab/relatedWorkOrContributorDate
1968-
http://library.link/vocab/relatedWorkOrContributorName
Parkinson, John
Series statement
  • Methods in molecular biology,
  • Springer protocols
Series volume
v. 533
http://library.link/vocab/subjectName
  • Nucleotide sequence
  • Expressed Sequence Tags
  • Gene Library
  • Nucleotide sequence
Label
Expressed sequence tags (ESTs : generation and analysis, edited by John Parkinson
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Expressed sequence tags : an overview / John Parkinson and Mark Blaxter -- Strategies for undertaking expressed sequence tag (EST) projects / Sandra W. Clifton and Makedonka Mitreva -- Construction of cDNA libraries : focus on protists and fungi / Naiara Rodríguez-Ezpeleta [and others] -- Generation of full-length cDNA libraries : focus on plants / Motoaki Seki [and others] -- Preparation of full-length cDNA libraries : focus on metazoans / Masako Harada and Yoshihide Hayashizaki -- Expressed sequence tags : normalization and subtraction of cDNA libraries / Marcelo Bento Soares [and others] -- Generating EST libraries : trans-spliced cDNAs / Cecilia Fernández and Rick M. Maizels -- Large-scale sequencing and analytical processing of ESTs / Makedonka Mitreva and Elaine R. Mardis -- EST processing : from trace to sequence / Ralf Schmid and Mark Blaxter -- Obtaining accurate translations from expressed sequence tags / James Wasmuth and Mark Blaxter -- EST databases and web tools for EST projects /Yao-Qing Shen [and others] -- Phylogenomic analysis of EST datasets / José M. Peregrín-Alvarez and John Parkinson -- Statistical analysis of expressed sequence tags / Edward Susko and Andrew J. Roger -- Application of ESTs in microarray analysis / Weizhong Li [and others] -- Exploiting ESTs in human health / Sandro José de Souza
Control code
316808074
Dimensions
unknown
Extent
1 online resource (x, 331 pages)
Form of item
online
Isbn
9781588297594
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-60327-136-3
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)316808074
Label
Expressed sequence tags (ESTs : generation and analysis, edited by John Parkinson
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Expressed sequence tags : an overview / John Parkinson and Mark Blaxter -- Strategies for undertaking expressed sequence tag (EST) projects / Sandra W. Clifton and Makedonka Mitreva -- Construction of cDNA libraries : focus on protists and fungi / Naiara Rodríguez-Ezpeleta [and others] -- Generation of full-length cDNA libraries : focus on plants / Motoaki Seki [and others] -- Preparation of full-length cDNA libraries : focus on metazoans / Masako Harada and Yoshihide Hayashizaki -- Expressed sequence tags : normalization and subtraction of cDNA libraries / Marcelo Bento Soares [and others] -- Generating EST libraries : trans-spliced cDNAs / Cecilia Fernández and Rick M. Maizels -- Large-scale sequencing and analytical processing of ESTs / Makedonka Mitreva and Elaine R. Mardis -- EST processing : from trace to sequence / Ralf Schmid and Mark Blaxter -- Obtaining accurate translations from expressed sequence tags / James Wasmuth and Mark Blaxter -- EST databases and web tools for EST projects /Yao-Qing Shen [and others] -- Phylogenomic analysis of EST datasets / José M. Peregrín-Alvarez and John Parkinson -- Statistical analysis of expressed sequence tags / Edward Susko and Andrew J. Roger -- Application of ESTs in microarray analysis / Weizhong Li [and others] -- Exploiting ESTs in human health / Sandro José de Souza
Control code
316808074
Dimensions
unknown
Extent
1 online resource (x, 331 pages)
Form of item
online
Isbn
9781588297594
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-60327-136-3
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)316808074

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