The Resource Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations, Aditi Khanna

Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations, Aditi Khanna

Label
Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations
Title
Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations
Statement of responsibility
Aditi Khanna
Creator
Contributor
Author
Degree supervisor
Subject
Genre
Language
eng
Summary
Candida albicans is an opportunistic fungus that is part of the normal flora of our gastrointestinal and urinary tracts, but can cause infections in immune compromised individuals. Fluconazole (FLC), which is a fungistatic azole, is a common drug used for treatment of C. albicans infection by targeting lanosterol demethylase (ERG11). Some clinical isolates show resistance to FLC, which can be attributed to an over expression of ABC transporters (CDR1, CDR2), Major Facilitator Transporter (MDR1), and the azole target (ERG11). Commonly the expression pattern of these genes is compared across strains of susceptible and resistant strains in the absence of drugs. However, gene expression levels of each strain may be different in different drug doses. This study investigates the pattern of gene expression of MDR1, CDR1, CDR2, and ERG11 in different C. albicans matched isolates at different doses of FLC. Along with the wildtype strain SC5314, isolates from a single patient with varying susceptibility to FLC were selected. The Minimum Inhibitory Concentration (MIC80) to FLC for each strain was determined by microbroth dilution. The mRNA expression of the genes was analyzed using Quantitative Real-Time PCR (qRT-PCR) at FLC concentrations 4-fold lower, 4- fold higher, and at their respective MICs. Gene expression was analyzed with and without FLC induction and the MDR1/CDR1/CDR2/ERG11 gene expression levels of all the strains were normalized to their uninduced expression levels. The experiments show that in resistant strains, the genes MDR1 and ERG11 are over expressed even without FLC induction, consistent with previous work. Increasing the FLC concentration does not have any significant effect on gene expression in resistant strains. For the azolesusceptible strains, the gene expression of CDR1 and CDR2 increases at FLC concentration below its MIC80 and declines at concentrations above the MIC80. Hence, the gene expressions of clinical isolates vary according to their MIC80 values
Cataloging source
UMK
http://library.link/vocab/creatorDate
1989-
http://library.link/vocab/creatorName
Khanna, Aditi
Degree
M.S.
Dissertation note
(Department of Biological Sciences).
Dissertation year
2015.
Granting institution
University of Missouri-Kansas City,
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
White, Theodore C.
http://library.link/vocab/subjectName
  • Candida albicans
  • Drug resistance
Label
Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations, Aditi Khanna
Instantiates
Publication
Note
  • "A thesis in Cell and Molecular Biology."
  • Advisor: Theodore C. White
  • Vita
Antecedent source
not applicable
Bibliography note
Includes bibliographical references (pages 17-18)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction -- Materials and methods -- Results -- Discussion
Control code
958683061
Dimensions
unknown
Extent
1 online resource (19 pages)
File format
one file format
Form of item
online
Level of compression
mixed
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
not applicable
Specific material designation
remote
System control number
(OCoLC)958683061
System details
  • The full text of the thesis is available as an Adobe Acrobat .pdf file; Adobe Acrobat Reader required to view the file
  • Mode of access: World Wide Web
Label
Expression pattern of drug-resistance genes in candida albicans at different fluconazole concentrations, Aditi Khanna
Publication
Note
  • "A thesis in Cell and Molecular Biology."
  • Advisor: Theodore C. White
  • Vita
Antecedent source
not applicable
Bibliography note
Includes bibliographical references (pages 17-18)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction -- Materials and methods -- Results -- Discussion
Control code
958683061
Dimensions
unknown
Extent
1 online resource (19 pages)
File format
one file format
Form of item
online
Level of compression
mixed
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations.
Quality assurance targets
not applicable
Specific material designation
remote
System control number
(OCoLC)958683061
System details
  • The full text of the thesis is available as an Adobe Acrobat .pdf file; Adobe Acrobat Reader required to view the file
  • Mode of access: World Wide Web

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