The Resource Functional study of Kcv potassium channel through manipultion [sic] of individual subunits, by Qiulin Tan, (electronic resource)

Functional study of Kcv potassium channel through manipultion [sic] of individual subunits, by Qiulin Tan, (electronic resource)

Label
Functional study of Kcv potassium channel through manipultion [sic] of individual subunits
Title
Functional study of Kcv potassium channel through manipultion [sic] of individual subunits
Statement of responsibility
by Qiulin Tan
Title variation
Functional study of Kcv potassium channel through manipulation
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Potassium channels make up a ubiquitous, physiologically essential class of integral membrane proteins. Many K+ channels contain four identical symmetrically associated subunits. To explore the role of an individual subunit in the channel functions, i.e. functional stoichiometry, one can genetically manipulate individual subunits and characterize the variation of channel functions with subunit composition. The functional stoichiometry of ion channels can be studied through the co-expression and concatemer methods. In this report, we utilized Kcv as a potassium model for functional stoichiometric study. The chlorella virus-encoded Kcv can form a homo-tetrameric potassium (K+) channel in the lipid membrane. This miniature peptide can be synthesized in vitro, and the tetramer purified from the SDS polyacrylamide gel retains the K+ channel functionality. Based on this capability, here we propose a simple, straightforward method for detecting the contribution from individual subunits to the channel functions. By using this approach, several mechanisms for ion permeation and potassium channel blocker binding regulated by subunit composition were identified. For example, the structural change from only one subunit in the selectivity filter G65C is sufficient to cause permanent channel dysfunction ("all-or-none" mechanism), whereas the mutation near the extracellular entrance L70Y additively modifies the ion permeation with the number of mutant subunits in the tetramer ("additive" mechanism). This study also demonstrates that four subunits of Kcv channel interact simultaneously with the potassium channel blocker tetraethylamonium (TEA) and each subunit contributes equally to the TEA binding free energy
Cataloging source
MUU
http://library.link/vocab/creatorDate
1972-
http://library.link/vocab/creatorName
Tan, Qiulin
Degree
Ph. D.
Dissertation year
2010.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Gu, Li-Qun
http://library.link/vocab/subjectName
  • Potassium channels
  • Chlorella
  • Plant viruses
Target audience
specialized
Label
Functional study of Kcv potassium channel through manipultion [sic] of individual subunits, by Qiulin Tan, (electronic resource)
Instantiates
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on May 28, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Li-Qun (Andrew) Gu
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
656426485
Extent
1 online resource (x, 101 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)656426485
Label
Functional study of Kcv potassium channel through manipultion [sic] of individual subunits, by Qiulin Tan, (electronic resource)
Publication
Note
  • Title from PDF of title page (University of Missouri--Columbia, viewed on May 28, 2010)
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Dissertation advisor: Dr. Li-Qun (Andrew) Gu
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
656426485
Extent
1 online resource (x, 101 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)656426485

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