The Resource GFP-based sensing and state estimation in transgenic plant cell culture, by Wei-Bin Lu

GFP-based sensing and state estimation in transgenic plant cell culture, by Wei-Bin Lu

Label
GFP-based sensing and state estimation in transgenic plant cell culture
Title
GFP-based sensing and state estimation in transgenic plant cell culture
Statement of responsibility
by Wei-Bin Lu
Title variation
Green fluorescent protein-based sensing and state estimation in transgenic plant cell culture
Creator
Subject
Language
eng
Summary
Plant cell cultures play an increasing important role in the development and production of newly discovered proteins. The development of plant-cell culture technology has been hindered by the lack of on-line sensing techniques for the biochemical activities. In this study, green fluorescent protein (GFP) based sensing techniques were developed and a Kalman filter was devised for state estimation from fluorescence measurement in transgenic plant cell cultures. To use GFP as a sensing mechanism for important cell culture variables such as protein and cell concentrations, functional relationships must be established between such variables and measured GFP fluorescence; in other words, sensing models must be developed. Transgenic tobacco cells were cultured in both flasks and a bioreactor. Measurements were made online or offline. Unattended GFP fluorescence was modeled as a function of GFP concentration, and inner filter and pH effects were accounted for as functions of cell concentration. As an application of the sensing models, a ratio method was developed to determine GFP concentration from fluorescence measurements. Various kinetic models for plant cell culture proposed in the literature were tested with measured data from both flask and bioreactor experiments. The Akaike's Information Criterion (AIC) and the Final Prediction Error (FPE) measure were used to assess the model performance. The Marquardt method was implemented and used for model parameter estimation and simulation. The results gave a model of good performance and low complexity. In the last part of the study, an extended Kalman filter (EKF) algorithm was successfully developed based on the kinetic model process and fluorescence measurement. The Kalman filter was used to estimate such import culture state variables as cell concentration, protein concentration and nutrient concentration. The estimations were compared with experimental data. The results show the effectiveness of the methodology. The results of this research established the usefulness of GFP as a sensing mechanism for cell culture property measurements. Measured GFP fluorescence coupled with the extended Kalman filter algorithm proved to be an effective methodology for state estimation in plant cell cultures
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1968-
http://library.link/vocab/creatorName
Lu, Wei-Bin
Degree
Ph. D.
Dissertation year
2002.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Transgenic plants
  • Green fluorescent protein
  • Plant cellular control mechanisms
  • Kalman filtering
  • Plant genetic engineering
Target audience
specialized
Label
GFP-based sensing and state estimation in transgenic plant cell culture, by Wei-Bin Lu
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 199-213)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
51555304
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 214 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
Label
GFP-based sensing and state estimation in transgenic plant cell culture, by Wei-Bin Lu
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 199-213)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
51555304
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 214 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • Engineering Library & Technology CommonsBorrow it
      W2001 Lafferre Hall, Columbia, MO, 65211, US
      38.946102 -92.330125
    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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