Coverart for item
The Resource Gene transfer and expression protocols, edited by E.J. Murray

Gene transfer and expression protocols, edited by E.J. Murray

Label
Gene transfer and expression protocols
Title
Gene transfer and expression protocols
Statement of responsibility
edited by E.J. Murray
Contributor
Subject
Language
eng
Member of
Is part of
Action
digitized
Cataloging source
YNG
Dewey number
574.87/322
Illustrations
illustrations
Index
index present
LC call number
QH448.4
LC item number
.G43 1991
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
no.7
Nature of contents
  • dictionaries
  • bibliography
NLM call number
ELECTRONIC BOOK
http://library.link/vocab/relatedWorkOrContributorName
Murray, E. J
Series statement
Methods in molecular biology
Series volume
v. 7
http://library.link/vocab/subjectName
  • Genetic transformation
  • Transfection
  • Gene expression
  • Gene Expression
  • Molecular Biology
  • Gene expression
  • Genetic transformation
  • Transfection
  • Regulering
  • DNA
  • Wetenschappelijke technieken
  • Transfert génétique
  • Transfection génétique
Target audience
adult
Label
Gene transfer and expression protocols, edited by E.J. Murray
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- Selection of cells defective in pyrimidine (TK−) and purine (APRT− and HPRT− salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou
Control code
232301991
Dimensions
unknown
Extent
1 online resource (xiii, 439 pages)
File format
multiple file formats
Form of item
online
Isbn
9781592594948
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896031780
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)232301991
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Gene transfer and expression protocols, edited by E.J. Murray
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- Selection of cells defective in pyrimidine (TK−) and purine (APRT− and HPRT− salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou
Control code
232301991
Dimensions
unknown
Extent
1 online resource (xiii, 439 pages)
File format
multiple file formats
Form of item
online
Isbn
9781592594948
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896031780
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)232301991
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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