Coverart for item
The Resource Generation of cDNA libraries : methods and protocols, edited by Shao-Yao Ying

Generation of cDNA libraries : methods and protocols, edited by Shao-Yao Ying

Label
Generation of cDNA libraries : methods and protocols
Title
Generation of cDNA libraries
Title remainder
methods and protocols
Statement of responsibility
edited by Shao-Yao Ying
Contributor
Subject
Genre
Language
eng
Member of
Cataloging source
DLC
Dewey number
611/.01816
Illustrations
illustrations
Index
index present
LC call number
QP624.5.A57
LC item number
G46 2003
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 221
Nature of contents
bibliography
http://library.link/vocab/relatedWorkOrContributorName
Ying, Shao-Yao
Series statement
Methods in molecular biology
Series volume
v. 221
http://library.link/vocab/subjectName
  • Antisense DNA
  • DNA, Antisense
  • DNA, Complementary
  • Genetic Techniques
  • Combinatorial Chemistry Techniques
  • Gene Library
Label
Generation of cDNA libraries : methods and protocols, edited by Shao-Yao Ying
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz ... [et al.] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jürgen Fülle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT(-)) cDNA for gene identification / San Ming Wang ... [et al.] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen ... [et al.] -- Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva ... [et al.] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying
Control code
51178265
Dimensions
24 cm
Extent
xiii, 335 pages
Isbn
9781588290663
Isbn Type
(alk. paper)
Lccn
2002192170
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
Label
Generation of cDNA libraries : methods and protocols, edited by Shao-Yao Ying
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Complementary DNA libraries: an overview / Shao-Yao Ying -- Rapid amplification of cDNA ends / Yue Zhang -- cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones -- Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen -- Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz ... [et al.] -- Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong -- Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara -- Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano -- cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji -- Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz -- Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin -- mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying -- Quality assessment of cDNA libraries / Hans-Jürgen Fülle -- Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying -- PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz -- Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin -- Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han -- Screening poly (dA/dT(-)) cDNA for gene identification / San Ming Wang ... [et al.] -- Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen ... [et al.] -- Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva ... [et al.] -- Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying -- Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin -- Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura -- Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge -- Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin -- Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge -- Future perspectives / Shao-Yao Ying
Control code
51178265
Dimensions
24 cm
Extent
xiii, 335 pages
Isbn
9781588290663
Isbn Type
(alk. paper)
Lccn
2002192170
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations

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