The Resource Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
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The item Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research
- Language
- eng
- Extent
- 1 online resource (xvii, 506 pages)
- Contents
-
- ""Glycoprotein Methods and Protocols: The Mucins""; ""Preface""; ""Contents""; ""Contributors""; ""PART I PURIFICATION OF MUCIN""; ""1 Isolation of Large Gel-Forming Mucins""; ""2 Preparation of Membrane Mucin""; ""PART II DETECTION AND QUANTITATION OF MUCIN""; ""3 Histologically Based Methods for Detection of Mucin""; ""4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods""; ""5 The Gastrointestinal Adherent Mucous Gel Barrier""; ""6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling""
- ""PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS""""7 Separation and Identification of Mucins and Their Glycoforms""; ""8 Heterogeneity and Size Distribution of Gel-Forming Mucins""; ""PART IV RHEOLOGY OF MUCIN""; ""9 Rheology of Mucin""; ""PART V MUCIN PEPTIDE ANALYSIS""; ""10 Amino Acid Analysis of Mucins""; ""11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation""; ""12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies""; ""13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation""
- ""PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS""""14 Monosaccharide Composition of Mucins""; ""15 O-Linked Oligosaccharide Chain Release and Fractionation""; ""16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides""; ""17 Measurement of Sulfate in Mucins""; ""PART VII MUCIN BIOSYNTHESIS""; ""18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study""; ""19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin""; ""20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis""
- ""21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing""""22 Inhibition of Mucin Glycosylation""; ""23 O-Linked Chain Glycosyltransferases""; ""PART VIII MUCIN GENE DETECTION""; ""24 Mucin cDNA Cloning""; ""25 Northern Blot Analysis of Large mRNAs""; ""26 Southern Blot Analysis of Large DNA Fragments""; ""27 In Situ Hybridization Techniques for Localizing Mucin mRNA""; ""28 Detection of Mucin Gene Polymorphism""; ""PART IX PREPARATION OF ANTIMUCIN ANTIBODIES""; ""29 Polyclonal and Monoclonal Techniques""; ""30 Monoclonal Antibodies to Mucin VNTR Peptides""
- ""PART X ENZYMATIC DEGRADATION OF MUCINS""""31 Mucinase Activity""; ""32 Proteinase Activity""; ""33 Glycosidase Activity""; ""34 Assays for Bacterial Mucin-Desulfating Sulfatases""; ""PART XI MUCIN-BACTERIAL INTERACTIONS""; ""35 Mucin-Bacterial Binding Assays""; ""36 Growth of Mucin Degrading Bacteria in Biofilms""; ""PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS""; ""37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping""; ""38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay""
- Isbn
- 9781280820403
- Label
- Glycoprotein methods and protocols : the mucins
- Title
- Glycoprotein methods and protocols
- Title remainder
- the mucins
- Statement of responsibility
- edited by Anthony P. Corfield
- Language
- eng
- Summary
- Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research
- Action
- digitized
- Cataloging source
- COO
- Dewey number
- 572/.68
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QP552.M8
- LC item number
- G59 2000
- Literary form
- non fiction
- NAL call number
- QH506
- NAL item number
- .M45 v. 125
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QU 25
- NLM item number
-
- ME9616J v.125 2000
- G5688 2000
- http://library.link/vocab/relatedWorkOrContributorName
- Corfield, Anthony P
- Series statement
- Methods in molecular biology
- Series volume
- v. 125
- http://library.link/vocab/subjectName
-
- Mucins
- Mucoproteins
- Glycoproteins
- Proteins
- Amino Acids, Peptides, and Proteins
- Mucins
- SCIENCE
- Mucines
- Glycoprotéines
- Mucins
- Wetenschappelijke technieken
- Molekularbiologie
- Methode
- Mucine
- Mucines
- Glycoprotéines
- Label
- Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- ""Glycoprotein Methods and Protocols: The Mucins""; ""Preface""; ""Contents""; ""Contributors""; ""PART I PURIFICATION OF MUCIN""; ""1 Isolation of Large Gel-Forming Mucins""; ""2 Preparation of Membrane Mucin""; ""PART II DETECTION AND QUANTITATION OF MUCIN""; ""3 Histologically Based Methods for Detection of Mucin""; ""4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods""; ""5 The Gastrointestinal Adherent Mucous Gel Barrier""; ""6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling""
- ""PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS""""7 Separation and Identification of Mucins and Their Glycoforms""; ""8 Heterogeneity and Size Distribution of Gel-Forming Mucins""; ""PART IV RHEOLOGY OF MUCIN""; ""9 Rheology of Mucin""; ""PART V MUCIN PEPTIDE ANALYSIS""; ""10 Amino Acid Analysis of Mucins""; ""11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation""; ""12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies""; ""13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation""
- ""PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS""""14 Monosaccharide Composition of Mucins""; ""15 O-Linked Oligosaccharide Chain Release and Fractionation""; ""16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides""; ""17 Measurement of Sulfate in Mucins""; ""PART VII MUCIN BIOSYNTHESIS""; ""18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study""; ""19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin""; ""20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis""
- ""21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing""""22 Inhibition of Mucin Glycosylation""; ""23 O-Linked Chain Glycosyltransferases""; ""PART VIII MUCIN GENE DETECTION""; ""24 Mucin cDNA Cloning""; ""25 Northern Blot Analysis of Large mRNAs""; ""26 Southern Blot Analysis of Large DNA Fragments""; ""27 In Situ Hybridization Techniques for Localizing Mucin mRNA""; ""28 Detection of Mucin Gene Polymorphism""; ""PART IX PREPARATION OF ANTIMUCIN ANTIBODIES""; ""29 Polyclonal and Monoclonal Techniques""; ""30 Monoclonal Antibodies to Mucin VNTR Peptides""
- ""PART X ENZYMATIC DEGRADATION OF MUCINS""""31 Mucinase Activity""; ""32 Proteinase Activity""; ""33 Glycosidase Activity""; ""34 Assays for Bacterial Mucin-Desulfating Sulfatases""; ""PART XI MUCIN-BACTERIAL INTERACTIONS""; ""35 Mucin-Bacterial Binding Assays""; ""36 Growth of Mucin Degrading Bacteria in Biofilms""; ""PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS""; ""37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping""; ""38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay""
- Control code
- 170167016
- Dimensions
- unknown
- Extent
- 1 online resource (xvii, 506 pages)
- Form of item
- online
- Isbn
- 9781280820403
- Lccn
- 99025785
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592590489
- Other physical details
- illustrations.
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)170167016
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
- Label
- Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- ""Glycoprotein Methods and Protocols: The Mucins""; ""Preface""; ""Contents""; ""Contributors""; ""PART I PURIFICATION OF MUCIN""; ""1 Isolation of Large Gel-Forming Mucins""; ""2 Preparation of Membrane Mucin""; ""PART II DETECTION AND QUANTITATION OF MUCIN""; ""3 Histologically Based Methods for Detection of Mucin""; ""4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods""; ""5 The Gastrointestinal Adherent Mucous Gel Barrier""; ""6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling""
- ""PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS""""7 Separation and Identification of Mucins and Their Glycoforms""; ""8 Heterogeneity and Size Distribution of Gel-Forming Mucins""; ""PART IV RHEOLOGY OF MUCIN""; ""9 Rheology of Mucin""; ""PART V MUCIN PEPTIDE ANALYSIS""; ""10 Amino Acid Analysis of Mucins""; ""11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation""; ""12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies""; ""13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation""
- ""PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS""""14 Monosaccharide Composition of Mucins""; ""15 O-Linked Oligosaccharide Chain Release and Fractionation""; ""16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides""; ""17 Measurement of Sulfate in Mucins""; ""PART VII MUCIN BIOSYNTHESIS""; ""18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study""; ""19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin""; ""20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis""
- ""21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing""""22 Inhibition of Mucin Glycosylation""; ""23 O-Linked Chain Glycosyltransferases""; ""PART VIII MUCIN GENE DETECTION""; ""24 Mucin cDNA Cloning""; ""25 Northern Blot Analysis of Large mRNAs""; ""26 Southern Blot Analysis of Large DNA Fragments""; ""27 In Situ Hybridization Techniques for Localizing Mucin mRNA""; ""28 Detection of Mucin Gene Polymorphism""; ""PART IX PREPARATION OF ANTIMUCIN ANTIBODIES""; ""29 Polyclonal and Monoclonal Techniques""; ""30 Monoclonal Antibodies to Mucin VNTR Peptides""
- ""PART X ENZYMATIC DEGRADATION OF MUCINS""""31 Mucinase Activity""; ""32 Proteinase Activity""; ""33 Glycosidase Activity""; ""34 Assays for Bacterial Mucin-Desulfating Sulfatases""; ""PART XI MUCIN-BACTERIAL INTERACTIONS""; ""35 Mucin-Bacterial Binding Assays""; ""36 Growth of Mucin Degrading Bacteria in Biofilms""; ""PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS""; ""37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping""; ""38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay""
- Control code
- 170167016
- Dimensions
- unknown
- Extent
- 1 online resource (xvii, 506 pages)
- Form of item
- online
- Isbn
- 9781280820403
- Lccn
- 99025785
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592590489
- Other physical details
- illustrations.
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)170167016
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Subject
- Amino Acids, Peptides, and Proteins
- Glycoproteins
- Glycoprotéines
- Glycoprotéines
- Laboratory manuals
- Methode
- Molekularbiologie
- Mucine
- Mucines
- Mucines -- analyse
- Mucins
- Mucins
- Mucins -- Laboratory manuals
- Mucoproteins
- Proteins
- SCIENCE -- Life Sciences | Biochemistry
- Wetenschappelijke technieken
Genre
Member of
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Glycoprotein-methods-and-protocols--the-mucins/RRoorIdAemk/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Glycoprotein-methods-and-protocols--the-mucins/RRoorIdAemk/">Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
