Coverart for item
The Resource Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield

Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield

Label
Glycoprotein methods and protocols : the mucins
Title
Glycoprotein methods and protocols
Title remainder
the mucins
Statement of responsibility
edited by Anthony P. Corfield
Contributor
Subject
Genre
Language
eng
Summary
Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research
Member of
Is part of
Action
digitized
Cataloging source
COO
Dewey number
572/.68
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP552.M8
LC item number
G59 2000
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 125
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.125 2000
  • G5688 2000
http://library.link/vocab/relatedWorkOrContributorName
Corfield, Anthony P
Series statement
Methods in molecular biology
Series volume
v. 125
http://library.link/vocab/subjectName
  • Mucins
  • Mucoproteins
  • Glycoproteins
  • Proteins
  • Amino Acids, Peptides, and Proteins
  • Mucins
  • SCIENCE
  • Mucines
  • Glycoprotéines
  • Mucins
  • Wetenschappelijke technieken
  • Molekularbiologie
  • Methode
  • Mucine
  • Mucines
  • Glycoprotéines
Label
Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • ""Glycoprotein Methods and Protocols: The Mucins""; ""Preface""; ""Contents""; ""Contributors""; ""PART I PURIFICATION OF MUCIN""; ""1 Isolation of Large Gel-Forming Mucins""; ""2 Preparation of Membrane Mucin""; ""PART II DETECTION AND QUANTITATION OF MUCIN""; ""3 Histologically Based Methods for Detection of Mucin""; ""4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods""; ""5 The Gastrointestinal Adherent Mucous Gel Barrier""; ""6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling""
  • ""PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS""""7 Separation and Identification of Mucins and Their Glycoforms""; ""8 Heterogeneity and Size Distribution of Gel-Forming Mucins""; ""PART IV RHEOLOGY OF MUCIN""; ""9 Rheology of Mucin""; ""PART V MUCIN PEPTIDE ANALYSIS""; ""10 Amino Acid Analysis of Mucins""; ""11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation""; ""12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies""; ""13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation""
  • ""PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS""""14 Monosaccharide Composition of Mucins""; ""15 O-Linked Oligosaccharide Chain Release and Fractionation""; ""16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides""; ""17 Measurement of Sulfate in Mucins""; ""PART VII MUCIN BIOSYNTHESIS""; ""18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study""; ""19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin""; ""20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis""
  • ""21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing""""22 Inhibition of Mucin Glycosylation""; ""23 O-Linked Chain Glycosyltransferases""; ""PART VIII MUCIN GENE DETECTION""; ""24 Mucin cDNA Cloning""; ""25 Northern Blot Analysis of Large mRNAs""; ""26 Southern Blot Analysis of Large DNA Fragments""; ""27 In Situ Hybridization Techniques for Localizing Mucin mRNA""; ""28 Detection of Mucin Gene Polymorphism""; ""PART IX PREPARATION OF ANTIMUCIN ANTIBODIES""; ""29 Polyclonal and Monoclonal Techniques""; ""30 Monoclonal Antibodies to Mucin VNTR Peptides""
  • ""PART X ENZYMATIC DEGRADATION OF MUCINS""""31 Mucinase Activity""; ""32 Proteinase Activity""; ""33 Glycosidase Activity""; ""34 Assays for Bacterial Mucin-Desulfating Sulfatases""; ""PART XI MUCIN-BACTERIAL INTERACTIONS""; ""35 Mucin-Bacterial Binding Assays""; ""36 Growth of Mucin Degrading Bacteria in Biofilms""; ""PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS""; ""37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping""; ""38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay""
Control code
170167016
Dimensions
unknown
Extent
1 online resource (xvii, 506 pages)
Form of item
online
Isbn
9781280820403
Lccn
99025785
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592590489
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)170167016
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Glycoprotein methods and protocols : the mucins, edited by Anthony P. Corfield
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • ""Glycoprotein Methods and Protocols: The Mucins""; ""Preface""; ""Contents""; ""Contributors""; ""PART I PURIFICATION OF MUCIN""; ""1 Isolation of Large Gel-Forming Mucins""; ""2 Preparation of Membrane Mucin""; ""PART II DETECTION AND QUANTITATION OF MUCIN""; ""3 Histologically Based Methods for Detection of Mucin""; ""4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods""; ""5 The Gastrointestinal Adherent Mucous Gel Barrier""; ""6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling""
  • ""PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS""""7 Separation and Identification of Mucins and Their Glycoforms""; ""8 Heterogeneity and Size Distribution of Gel-Forming Mucins""; ""PART IV RHEOLOGY OF MUCIN""; ""9 Rheology of Mucin""; ""PART V MUCIN PEPTIDE ANALYSIS""; ""10 Amino Acid Analysis of Mucins""; ""11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation""; ""12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies""; ""13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation""
  • ""PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS""""14 Monosaccharide Composition of Mucins""; ""15 O-Linked Oligosaccharide Chain Release and Fractionation""; ""16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides""; ""17 Measurement of Sulfate in Mucins""; ""PART VII MUCIN BIOSYNTHESIS""; ""18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study""; ""19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin""; ""20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis""
  • ""21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing""""22 Inhibition of Mucin Glycosylation""; ""23 O-Linked Chain Glycosyltransferases""; ""PART VIII MUCIN GENE DETECTION""; ""24 Mucin cDNA Cloning""; ""25 Northern Blot Analysis of Large mRNAs""; ""26 Southern Blot Analysis of Large DNA Fragments""; ""27 In Situ Hybridization Techniques for Localizing Mucin mRNA""; ""28 Detection of Mucin Gene Polymorphism""; ""PART IX PREPARATION OF ANTIMUCIN ANTIBODIES""; ""29 Polyclonal and Monoclonal Techniques""; ""30 Monoclonal Antibodies to Mucin VNTR Peptides""
  • ""PART X ENZYMATIC DEGRADATION OF MUCINS""""31 Mucinase Activity""; ""32 Proteinase Activity""; ""33 Glycosidase Activity""; ""34 Assays for Bacterial Mucin-Desulfating Sulfatases""; ""PART XI MUCIN-BACTERIAL INTERACTIONS""; ""35 Mucin-Bacterial Binding Assays""; ""36 Growth of Mucin Degrading Bacteria in Biofilms""; ""PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS""; ""37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping""; ""38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay""
Control code
170167016
Dimensions
unknown
Extent
1 online resource (xvii, 506 pages)
Form of item
online
Isbn
9781280820403
Lccn
99025785
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592590489
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)170167016
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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